| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,975,253 | 0 | A | C | 56.2% | 3.6 / 12.7 | 17 | Q688P (CAG→CCG) | sasA_19 | Adaptive‑response sensory‑kinase SasA |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base C (6/3); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGC > KT2440/5975119‑5975385 | tGGCGGGGGCTGCCTGCCCCCGGGCCAGAACGGCTGGGGCTTCACTCCTGTCCGGGTGATGGAGGCCGCTTATGCACGCACGCCCGTGCACCAGCGCGCCTCGCTGCAGGCACGTATCGCCCTGGCCATGCGCCa < 5:245946/135‑1 (MQ=255) tCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTTGCTGCATGCACGTATCGCCCCGGCCAATCGCCCCGC‑GTGGcccc > 4:151197/1‑133 (MQ=255) tgtGGGCTCCACTATTGCCGGGGTGTGGGCGGCCGGTTAGCCACCCCCCCCCGGCCCCCCGCGCGCGGGGCTGAATGCAGTTATCGCCGGGCCAATGCCCCTGGC‑TTGGCCTGGGCGAACGCACGAGGTgttgtt < 4:176276/133‑1 (MQ=255) gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGGCAAGCGCCCCGCGTTGGCCAGGG‑GCACGCACGAGGGggtgttggg > 4:290980/1‑132 (MQ=255) gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCCGGCCCGGCGCCCCGC‑TGGGCCCCGGCGCACGCCCGAGGGgtggttggc > 4:226642/1‑132 (MQ=255) tCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCATGCGCCCGGC‑TTGGCCAGGGCGCCCGCACGCGGGGGTGTTCGAGCTCgcccg > 3:270191/1‑133 (MQ=255) cGGGTGAGGGAGGCCGCTAAGGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGCCCATCCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTa < 8:106184/135‑1 (MQ=255) ggAGGCCGGTTTTGCACTCACGCCCGGGCACCTGCGCGCGTCGCTGCATGCAGGTATCGCCCTGGCCTTGCGCCCGGG‑TTGGCCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCTAGCTACAAcgcg < 4:263816/134‑1 (MQ=255) gCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGGCCCGGCCCAGGGCCCGGG‑GTGGGCCGGGCGCCCGCCCGCGGGGTTGTTGGCGCGGGCCCGCCCGGGACCCCGCGCCCGcccc > 3:126224/1‑135 (MQ=255) cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGGCCAGGCCCAGCCGCCCGC‑GTGGCCCGGGGGCGCCCCCGAGGGGTTGTTGGGGCTCGGCAGCCCGCGACAACCCGCCCGCCCCGGCGGGGTTGGAGCg > 4:218807/1‑135 (MQ=255) cgcgcgcCTCGCTGCATGCACGTAGCGCCCTGGCCATGCGCCAGGC‑TGGGCCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCg < 4:15735/134‑1 (MQ=255) aGCGCGCCGCGCTGCACGCACTTCTCGCCCGCCCCCCGCCCCCGGG‑TTGCCCCGGGCGAACGCACGAGGTGTTGATCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCg < 7:38785/135‑1 (MQ=255) cgcgccgcgcGGCATCCACGTAGCGCCCGGGCCATGCGCCCGGC‑TGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGaa < 1:100975/135‑1 (MQ=255) gTAGCGCCCTGGCCATGCGCCAGGC‑TGGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTAc < 2:236873/135‑1 (MQ=255) ccAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCtttt > 8:150451/1‑114 (MQ=255) ccAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCtttt < 7:150451/114‑1 (MQ=255) ccAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc > 7:310364/1‑135 (MQ=255) ccAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc > 2:204591/1‑135 (MQ=255) | TGGCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGC‑TTGGCCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGC > KT2440/5975119‑5975385 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |