Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	5,757,239	(A)6→5	coding (781/834 nt)	CJ019_05400 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,757,234	0	A	.	92.9%	164.4 / 6.7	44	coding (776/834 nt)	CJ019_05400	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (26/13);  minor base C (0/3);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 4.88e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGC  >  KT2440/5757102‑5757364                                                                                                                                     |                                                                                                                                   aGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaaaaa                                                                                                                                >  1:219525/1‑137 (MQ=255)   cTGCACGCGCGCGAACAGGACCAGGCCGACCGGGCCACGGATTTGACGACCATCGAGCAAACCCTCGCCCGCCCGGCCCGTCAAGCTGAAGAGGCACGCAAGAAGGCCCTGCGGGCGCAGCAGGAAGCGGaaaaacg                                                                                                                              <  2:152079/137‑3 (MQ=255)    tGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGaaaaacgc                                                                                                                             <  1:251189/137‑4 (MQ=255)             gcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGCAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGACCTGCTGGCGCAGCAGGAAGCGG‑AAACACGCCGCcagcag                                                                                                                   >  6:167988/1‑137 (MQ=255)                     gACCAGGTCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCt                                                                                                           <  5:348906/137‑1 (MQ=255)                        ccggCCCACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCGGTTAAGCTGACGCGCCACGCAAGATGGCCCTGCTGGCGCTGCAGGAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGgc                                                                                                        <  1:301712/135‑1 (MQ=255)                        cAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgc                                                                                                        >  1:395203/1‑137 (MQ=255)                        cAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgc                                                                                                        >  6:334493/1‑137 (MQ=255)                          ggCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccg                                                                                                      >  2:373288/1‑137 (MQ=255)                             cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGACCAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGgccgccg                                                                                                   <  4:47426/137‑1 (MQ=255)                                  tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGACGCGCTGGCCGCAGCCGCt                                                                                              >  1:306076/1‑137 (MQ=255)                                  tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCt                                                                                              >  8:374932/1‑137 (MQ=255)                                  tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCt                                                                                              >  6:273222/1‑137 (MQ=255)                                  tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCt                                                                                              >  3:310513/1‑137 (MQ=255)                                   gcccAAGGTCTTGAAGACCATCGAGCAACCCCTCGCCCGCCTGGCCCGTCAAGCTGAAGTGCCACGCAAGACGCCCCTGCTGGCGCAGCAGGAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTg                                                                                             <  4:174259/135‑1 (MQ=255)                                       aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGTGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcg                                                                                         >  5:57545/1‑137 (MQ=255)                                       aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcg                                                                                         >  5:193942/1‑137 (MQ=255)                                         ggTCTTGAAGCCCATCGCGGAAACCCGCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCGGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgcg                                                                                       <  6:279250/137‑1 (MQ=255)                                           tCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAc                                                                                     <  2:50014/137‑1 (MQ=255)                                           tCTTCAACACCATCGAGCAACCCCGCCCCCGCCCGGCCCTTTAAGCTGACCCGGCCCGAAACACGCCCCGGGGGGTGAAGATGTAAGC‑GCAAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAc                                                                                     <  6:57545/137‑1 (MQ=255)                                                aaGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                                <  8:122853/137‑1 (MQ=255)                                                        cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                        >  2:232333/1‑137 (MQ=255)                                                          aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCt                                                                      >  8:350468/1‑137 (MQ=255)                                                          aGGAAACCCTCGCACGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCt                                                                      >  7:373657/1‑137 (MQ=255)                                                                    cgcccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAg                                                            >  2:315104/1‑137 (MQ=255)                                                                       ccgccAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGCGCCTGCCGAGCCAGTgg                                                         >  6:361890/1‑137 (MQ=255)                                                                               cccGTGAAGGTGACCAGGCACGCAAGACGGCCCTGCTGGGGCAGCAGGATGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCGGCCGCCGCTGCCCTCGACATGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                                 <  7:130987/137‑1 (MQ=255)                                                                               cccGTGAAGCTGAAGAGCCACCCAAGACGGCCCTGCTGGCGCAGCAGGAAGC‑GCAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                                 <  1:59143/137‑1 (MQ=255)                                                                                    gaagcggaagcgCCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                            <  6:88998/137‑1 (MQ=255)                                                                                          caaGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGTCCCg                                      <  7:92791/136‑1 (MQ=255)                                                                                           aaGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGt                                     >  6:278913/1‑137 (MQ=255)                                                                                             gagGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCTCCAGCAGGAAGCGCTCGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCATAGGAACCGCCGAAAAAGGacctaac                                   >  6:58453/1‑133 (MQ=255)                                                                                                ccACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac                                <  4:107085/136‑1 (MQ=255)                                                                                                ccACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGC‑GCAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac                                <  3:65565/136‑1 (MQ=255)                                                                                                     cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCg                           >  4:302637/1‑137 (MQ=255)                                                                                                         aagCCCCTGCTGGCGCAGGAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGcc                       <  5:394252/137‑1 (MQ=255)                                                                                                              ccTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATgg                  >  3:249108/1‑137 (MQ=255)                                                                                                                 gctgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt               >  2:163407/1‑137 (MQ=255)                                                                                                                   tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTcc             >  5:133835/1‑137 (MQ=255)                                                                                                                   tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTcc             >  3:73824/1‑137 (MQ=255)                                                                                                                   tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTcc             >  3:385406/1‑137 (MQ=255)                                                                                                                   tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGGTTcc             >  7:163382/1‑137 (MQ=255)                                                                                                                     gcgcAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAg           <  5:273222/137‑1 (MQ=255)                                                                                                                         agcagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGAt       >  1:224096/1‑137 (MQ=255)                                                                                                                          gcagGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATg      <  2:31013/137‑1 (MQ=255)                                                                                                                            cggAAGCGG‑GAAAACGCCGCCACCAGGATGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgc    <  5:192733/136‑1 (MQ=255)                                                                                                                              gAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGgcgc  >  5:214596/1‑137 (MQ=255)                                                                                                                                     |                                                                                                                                   AGCTGCAAGCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCGC  >  KT2440/5757102‑5757364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑