| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,537,415 | 0 | C | G | 38.1% | 20.4 / 11.4 | 21 | A388G (GCG→GGG) | mdfA | Multidrug transporter MdfA |
| Reads supporting (aligned to +/- strand): ref base C (12/1); new base G (0/8); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGTTCTCCAGCGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCT > KT2440/1537280‑1537541 | tGTTCTCCAGCGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGgcggcg > 1:121861/1‑137 (MQ=255)tGTTCTCCAGCGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGgcggcg > 5:120773/1‑137 (MQ=255) cGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCgg > 1:236074/1‑137 (MQ=255) cGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCgg > 3:155933/1‑137 (MQ=255) agagCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑Cg > 7:366853/1‑137 (MQ=255) agagCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑Cg > 1:386919/1‑137 (MQ=255) ggggCTGGTTTCGGCCATGATGGTGATCAGACCGATGGCCTTGGTGGGTTTGGGCGGCGCGCTGCTGGCGATGATTGGCGCGGTTGCCAGTTTGATGAATTTTGCCT‑TGGCGGGGGGGGTGGCGGGGG‑CGTTGgc‑gc < 2:379277/137‑1 (MQ=255) tttCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCg > 6:278625/1‑137 (MQ=255) cATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCGCTGTGGt > 8:165596/1‑137 (MQ=255) aTGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCAATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCGCTGTGGtt > 8:273184/1‑137 (MQ=255) tGGGGATGATCACGATAGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCGCTGTGGTTtgtg > 1:28891/1‑137 (MQ=255) atgatCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCGCTGTGGTTTGTggtggg > 6:224176/1‑137 (MQ=255) cACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCGCTGTGGTTTGTGGTGGGTGggc > 4:186386/1‑137 (MQ=255) ttGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGa > 1:38001/1‑137 (MQ=255) gggTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAggggg > 1:51093/1‑136 (MQ=255) gctgatttttGCCG‑GGGGGGGGGGGGGGGGGGGGG‑CGTTGGC‑GCGGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 2:3164/131‑1 (MQ=255) tttttttttGCCCGGGGGGGGGGGGGGGGGGGGGG‑CGTGGGC‑GGGGTGGCGGGTGTGGTTTGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 5:278625/132‑1 (MQ=255) ggtgcttttGCCCGGGGGGGGGGGGGGGGGGGGGG‑GGTTGGC‑GCGGTGGCCGCGGTGGTTTGGGGGGGGGGGGCGGCCGGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 7:320331/132‑1 (MQ=255) ggtgaTTTTGCCCGGGGGGGGGGGGGGGGGGGGGG‑GGTGGGC‑GCGGGGGCGGGGGTGGTTTGGGGGGGGGGGGGGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 3:269442/133‑1 (MQ=255) ggggaTTTTGCCC‑CGGGGGGGGGGGGGGGGGGGG‑GGGTGGCGGCGGGGGCGGCTGTGGTTTGTGGTGGGGGGCCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTaa < 1:367271/133‑1 (MQ=255) ttttGCCCGGGGGGGGGGGGGGGGGGGGGG‑CGGTGGC‑GCGGTGGCCGGTGTGGTTTGTGGGGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCg < 4:8757/137‑1 (MQ=255) ttttGCCC‑GGGGGGGGGGGGGGGCGGGGGCCGTTGGC‑GCGGTGGCCGCTGTGGTTTGTGGTGGGGGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCg < 6:161346/137‑1 (MQ=255) cccgtGGGGGCGGGTGGGGGGGGGG‑CGTTGGC‑GGTGGGGCGGGTGTGGGTGGTGGGGGGGGGGCGGCCTGGGGAAGGGGCTGGAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCt < 7:335247/133‑1 (MQ=25) | TGTTCTCCAGCGAACAGAGCAAGGGGCTGGTTTCGGCCATGCTGGGGATGATCACGATTGCCTTGCTGGGGTTTGGCGGAGCGCTGCTGGCGATGATTGGCGCCGGTGCCAGCTTGCTGCATTTTGCCC‑TGGCGGCGGGGGTGGCGGGGG‑CGTTGGC‑GCTGTGGCCGCTGTGGTTTGTGGTGGGTGGGCGGCCTGGGGAAGGGGCTGTAGCTCGATAATCGGTATTGCCGGGGCCGGCCTCTTCGCGGGTAAACCCGCTCCT > KT2440/1537280‑1537541 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |