| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 690,150 | T→G | intergenic (+27/‑2432) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 690,150 | 0 | T | G | 76.5% | 17.1 / ‑2.8 | 20 | intergenic (+27/‑2432) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base T (4/0); new base G (13/0); total (20/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGG‑CCTCCCCGC‑TTTG‑T‑TTTTG‑GGGGTTGGTTGTTGCTGT‑TGGGGAGGAACTGTT > KT2440/690021‑690206 | cccGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCacggcg > 2:122132/1‑132 (MQ=255) cGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGTCCGG‑CCTcc > 7:375758/1‑137 (MQ=255) tGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGAAGG‑GCGcccccc > 8:360566/1‑135 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCGG‑CCGccccccg > 5:151110/1‑134 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCCTGACG‑CCGCCCCGcg > 8:120232/1‑136 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGCCCGG‑CCGCCCCCG‑GTTG‑T‑TTTTg‑ggggg > 2:285819/1‑136 (MQ=255) aCCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCCCG‑GGTCCCCCCGGTTG‑T‑TTTTg‑ggggg > 4:172515/1‑136 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCGGACGCG‑CGCGCCCCC‑TTTG‑T‑TTTTG‑GGGTTGGggtgt > 2:46714/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGTCACG‑CCTCCCCGC‑GGTG‑T‑TTTTT‑GGGGTtgtttgtt > 8:294563/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCAGG‑GCGGCCCGC‑GTTG‑TGTTTTG‑GGGGTGGgttgt > 4:346179/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCAGG‑GCGGCCCCC‑GGTGTT‑TTTTT‑GGggttggtgtt > 3:312112/1‑134 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGCCGG‑CCCACCCGC‑GTTG‑T‑TTTTGTGGGggtggtggt > 8:5348/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGCGGGCCGGCCCCCCCCCC‑GGGG‑T‑TTTTG‑GGGggtggtggt > 7:165056/1‑137 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGAGG‑CCGCCCCGC‑GTTG‑T‑TTTTG‑GGGGGTGGgtgttgg > 6:182401/1‑136 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGGCCTCACG‑CCGCCCCCC‑ATTT‑A‑TTTTG‑GGGGTGTGtggttgg > 6:354146/1‑136 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGGGG‑GCGCCCCCC‑GGTG‑T‑TGTTT‑GGGGGTGGGTGTTTGTGT‑TGgggggggggtt > 2:248572/1‑132 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCGG‑GCGCCCCGC‑GGTG‑T‑GTTTT‑GGGGGGGGGTGGTGGGGT‑TGgggggggaggt > 7:145848/1‑132 (MQ=255) ttGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGCCGGG‑CGCCCCCCG‑TTTT‑T‑TTTTG‑GGGGGT‑GTTGTTGGTGT‑TGGGGGGGGACGGt > 7:321568/1‑137 (MQ=255) ttGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCCG‑CCCCCCCCC‑GTTGTT‑TTTTG‑GGGGTTGG‑TGTTGGGGT‑TGGGGGGGGACGGt > 7:283703/1‑137 (MQ=255) tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGGCGGAGG‑CCCCCCCCC‑CGTG‑T‑TTTTT‑GTGGTTGGTTTTTG‑TGTGTGGGGGGGGACTggt > 5:125775/1‑135 (MQ=255) | CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGG‑CCTCCCCGC‑TTTG‑T‑TTTTG‑GGGGTTGGTTGTTGCTGT‑TGGGGAGGAACTGTT > KT2440/690021‑690206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |