Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,378,944 T→G 29.3% N189T (AAC→ACC)  oprD_6 ← Porin D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,378,9440TG29.3% 55.5 / 4.5 41N189T (AAC→ACC) oprD_6Porin D
Reads supporting (aligned to +/- strand):  ref base T (11/17);  new base G (10/3);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 4.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGTTGACGTTGGCGTAGTATTTGCGCCAGTAGTCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCTGGTGATCAGGGTGCCCTGGGCGATTTCTGGCAGCAGACGGCTGTCATCGGTGGCGAAGACCG  >  KT2440/1378811‑1379076
                                                                                                                                      |                                                                                                                                    
ccAGTTGAAGTTGGCGTAGTATTTGCACCAGTAGGCTTCACCCTTCTAGTAGTACAGGCTGGGGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑ggtt                                                                                                                                     <  1:8956/135‑1 (MQ=255)
                 agtaTTTGCGCCAGTAGTCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAg                                                                                                                    >  2:195531/1‑135 (MQ=255)
                      ttGCGCCAGTAGTCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGGCGGTGAAGGCGGAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAgg                                                                                                               <  4:181477/135‑1 (MQ=255)
                       tGCGCCAGTAGTCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAgg                                                                                                               <  2:162554/134‑1 (MQ=255)
                          gcCAGAAGTCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGGTTGTTACTGGGCAGTCCGGCTgg                                                                                                           >  1:168201/1‑135 (MQ=255)
                                agtCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTTTGTTGCTGGGCAGTCAGGCTGGTGAAGt                                                                                                     >  5:216606/1‑135 (MQ=255)
                                 gtCTTCAACCTTCGAGTCGTACAGGCTGGTGCTGAGGTGGTCGGTGAAGGCGTAGGAGCCGCAGACCAGGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGGTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGa                                                                                                   <  7:177704/136‑1 (MQ=255)
                                  tCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGACGGCGTAGGTGCCGCCGACCACGTTGTCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGa                                                                                                   <  5:23898/135‑1 (MQ=255)
                                  tCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGa                                                                                                   <  4:186774/135‑1 (MQ=255)
                                  tCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTg                                                                                                    <  3:170299/134‑1 (MQ=255)
                                  tCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGGAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTg                                                                                                    <  5:180880/134‑1 (MQ=255)
                                   cTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGTGGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGAc                                                                                                  <  5:63908/135‑1 (MQ=255)
                                   cTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGAc                                                                                                  <  1:195531/135‑1 (MQ=255)
                                       aaCCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTTTGTTACTGGGCAGTCAGGCTGGTGAAGTTACCAGc                                                                                              >  5:82938/1‑135 (MQ=255)
                                        aCCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGGGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGc                                                                                              <  6:190922/134‑1 (MQ=255)
                                               agtagtACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTGTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAggg                                                                                      >  2:76035/1‑135 (MQ=255)
                                                gtagtaCAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGGGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGt                                                                                     <  6:164543/135‑1 (MQ=255)
                                                            ggTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGCGGGTTGGTTTGTTCCTGGGCAGTCAGGCGGGGGAAGTGCCCAGCGTGCAGGGTCAGCCCTTGGa                                                                          >  8:254407/1‑135 (MQ=255)
                                                            ggTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTTTGTTCCTGGGCCGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGCtc                                                                        >  7:182261/1‑136 (MQ=255)
                                                              tGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGAtct                                                                       <  5:139691/135‑1 (MQ=255)
                                                              tGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTTTGTTGCTGGGCAGTCAGGCTGGTGAAGTGCCCAGCGTGCAGGGTCCGCCCGTCGAtct                                                                       >  5:101976/1‑135 (MQ=255)
                                                              tGCTGAGGGGGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTTTGTTCCTGGGCAGGCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGAtct                                                                       <  1:163394/135‑1 (MQ=255)
                                                                cTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTCGGCAGACAGGCTGGTGAACTTACCAGCGTGCAGGGTCAGCCCTTCGAtaacgt                                                                    >  7:273002/1‑131 (MQ=255)
                                                                 tGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑ggttg                                                                                                                                    >  4:180092/1‑71 (MQ=255)
                                                                 tGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑ggttg                                                                                                                                    <  3:180092/71‑1 (MQ=255)
                                                                 tGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTACTGGGCCGGCAGGCTGGTGAAGTTACCAGCGTGCAGGGTCAGGCCGTCGATCTCGt                                                                    >  6:243891/1‑135 (MQ=255)
                                                                 tGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGGTTGTGGCTGGGCAGTCCGGCTGGTGTAGGGACCAGCGTGGAGGGTCAGGCCCTGGCTCTCGt                                                                    >  5:18403/1‑135 (MQ=255)
                                                                   aGGTTGTCGGTGCAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGtt                                                                   <  8:109638/134‑1 (MQ=255)
                                                                    ggTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGtt                                                                   <  4:176849/133‑1 (MQ=255)
                                                                      ttGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCCGCGTGCAGGGTCCGCCCTTCGATCTCGTTGCTg                                                               >  4:254179/1‑135 (MQ=255)
                                                                      ttGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGGGTGTTGCTGGGGCGTCAGGCTGGTGGAGTGCCCCGCGTGGAGGGTCCGCCCATCGCTCTCGGTGCTg                                                               >  4:57652/1‑135 (MQ=255)
                                                                      tggTCGGTGAAGGCGTAGGGGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCTgg                                                              <  8:235959/134‑1 (MQ=255)
                                                                       tGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCTgg                                                              <  2:82785/135‑1 (MQ=255)
                                                                        ggcGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCTGGt                                                             <  5:233831/133‑1 (MQ=255)
                                                                          cGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCCGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCGGGTGAt                                                          >  7:143624/1‑136 (MQ=255)
                                                                          cGGTGAAGGCGTAGGTGCCGCCGACCACGTNGGCTTCCNTCAGGCCGAAGCTGTCGC‑GGTTTGNTTGTTCCTGGGCAGTCAGGCTGGTGAAGNGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTTCTGGTGa                                                           >  7:147488/1‑135 (MQ=255)
                                                                           ggTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGCCCCGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCTGGTGa                                                           >  4:130652/1‑134 (MQ=255)
                                                                           ggTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGGTGGTTTGTTACGGGGCAGTCAGGCTGGTGAACTGACCAGCGTGCAGGGTCAGGCCGTCGATGTCGGTGCTGGTGAt                                                          >  5:52541/1‑135 (MQ=255)
                                                                                  gCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTGCTGGGCAGTCAGGCTGGTGTAGTGAACAGCGGGCAGGGTGACCCCCTCGATCTCGGTGCTGGTGGTCAGGGTg                                                   >  2:251265/1‑135 (MQ=255)
                                                                                                                       cGAAGCTGTCGC‑GGGTGGTTTGTTACTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTGGTGGTGATCAGGGTGCCCGGGGCGCTTTATGGCATCAGACCGCTGTGATAgg              >  2:213103/1‑135 (MQ=255)
                                                                                                                            cTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCTGGTGATCAGGGTGCCCTGGGCGATTTCTGGCAGCAGACGGCTGTCATCGGTGGCg         <  1:251265/135‑1 (MQ=255)
                                                                                                                             tGTCGC‑GGGTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCCGCGTGCCGGGTCAGCCCTTCGAGCTCGTGGCGGGGGATCATGGTGCCCTGGGCGCTTTCGTGCAGCCGTCGGCTGGGATCGGTgtc          >  6:28367/1‑131 (MQ=255)
                                                                                                                                  c‑gGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCTGGTGATCAGTGTGCCCTGGGCGATTTCTGGCAGCAGACGGCTGTCATCGGTGGCGAAGACCg  <  8:273002/136‑1 (MQ=255)
                                                                                                                                  c‑gGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCTGGTGATCAGGGTGCCCTGGGTGATTTCTGGCAGCAGACGGCTGTCATCGGTGGCGAAGAcc   >  2:69227/1‑135 (MQ=255)
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CCAGTTGACGTTGGCGTAGTATTTGCGCCAGTAGTCTTCAACCTTCGAGTAGTACAGGCTGGTGCTGAGGTTGTCGGTGAAGGCGTAGGTGCCGCCGACCACGTTGGCTTCCTTCAGGCCGAAGCTGTCGC‑GGTTGGTTTGTTCCTGGGCAGTCAGGCTGGTGAAGTGACCAGCGTGCAGGGTCAGCCCTTCGATCTCGTTGCTGGTGATCAGGGTGCCCTGGGCGATTTCTGGCAGCAGACGGCTGTCATCGGTGGCGAAGACCG  >  KT2440/1378811‑1379076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑