Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	96,480	T→G	32.1%	intergenic (+121/‑72)	cynT → / → CJ019_00104	Carbonic anhydrase 1/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	96,480	0	T	G	32.1%	34.2 / 2.2	40	intergenic (+121/‑72)	cynT/CJ019_00104	Carbonic anhydrase 1/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (10/15);  new base G (8/6);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGT  >  KT2440/96345‑96604                                                                                                                                        |                                                                                                                               agagGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCt                                                                                                                                <  7:39033/136‑1 (MQ=255)   agGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgc                                                                                                                              <  7:211679/136‑1 (MQ=255)    ggCCGCTACTACGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTgc                                                                                                                              <  3:45920/135‑1 (MQ=255)          tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCtt                                                                                                                        <  5:86531/135‑1 (MQ=255)          tactaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTg                                                                                                                       >  8:10565/1‑136 (MQ=255)            ctaAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCGTCGGCGGCCGTGAATTCCCTGCCTGCCCTGCCGGCGTGCGCGCTGGCGGCCCCCGCCTGCGCGCtggg                                                                                                                      >  6:189475/1‑132 (MQ=255)                       gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGGCTTGAATTACCTGACTGCCCTGCCGGCGTGCTCGCAGGCGGCCCGCCGCGGCCCGCGTGTACAGGGCCaaa                                                                                                           >  1:2984/1‑135 (MQ=255)                       gTCCGCCGCTAGAACCTTGATAGCCGGCTGCACACTTCAGGGGTGTGGCGCGGCCATCGGCTGCCATGAATTACCATACTGCCCTGACGGCGTGCTCGCTGTCGGCCCGCGCCATCGCCCATGT‑CATGGGGaaa                                                                                                           >  6:144022/1‑134 (MQ=255)                         ccgccgCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGGCTTGGATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGT‑GCGGGGGAAAccg                                                                                                        >  6:205709/1‑133 (MQ=255)                         ccgccgCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGT‑CAGGGGGAAACGt                                                                                                        >  6:56946/1‑135 (MQ=255)                          cgccgcTAGAACCTTGATAGCCGGCTGCACCCTTCTGGGGTGGGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGGGTGCGCGCTGGCGGCCCGCGCCTGCGCGCTTGT‑CCGGGGCAAACGTg                                                                                                       <  3:195954/135‑1 (MQ=255)                          cgccgcTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGGGTGGCGCGGCCTTCGGCTGCCTTGATTTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGGGCTTGT‑CTGGGGCAAACGTg                                                                                                       <  4:74475/135‑1 (MQ=255)                            ccgcTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCGTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGT‑CAGGGGCAAACGTG‑cc                                                                                                    >  6:107579/1‑135 (MQ=255)                                 aGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCGTGT‑CAGGGGCAAACGTG‑CCCACgg                                                                                               >  4:195954/1‑135 (MQ=255)                                             gCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATgg                                                                                   <  7:10565/135‑1 (MQ=255)                                                                ggTGTGGGGCGGCCTTGGGTTGCCTTTATTTCCCTGCCTGCCCTGCGGGGGTGCTGGGTGGCGGCCCGCGCGGGGGCGGTTGT‑CTGGGGCAAAGGTC‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAgg                                                                <  3:154144/135‑1 (MQ=255)                                                                 gtgtGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCATGGGAATTGCCGTGCGACTAGAGAAAGga                                                               <  4:258986/135‑1 (MQ=255)                                                                       cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCGTGT‑GAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACGAGAGAAAGGAGAAaca                                                         >  1:158350/1‑135 (MQ=255)                                                                       cgcgGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCCCGCCGGCGCGCGTGT‑GAGGGGCAAAACGG‑CCCCCCGCCCCGGGCATGGCCGGGCGGCCAGACAAAAGAGcaaac                                                         >  6:236247/1‑133 (MQ=255)                                                                       cgcgGCCTTCGGCTGCCGTGAATTACCTGACTGCCCTGCCGGCGTGCGAGCTGGCGGCCCGCCCCGGCGCGCTTGT‑GACGGGCAAACCTG‑CCCCCCGCCAGGGGGATTGGCGGGCGCCTAGAGAAAGGAGaaaaa                                                         >  4:216666/1‑133 (MQ=255)                                                                        gggggcTTCGGCGGCCTTGAATTCCCTGCCCGCCCTGCCGGGGTGGTCGGGGGCGGCCGGGGCCGGCGGGGTTGT‑CCGGGG‑AAACGGGCCCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                        <  5:156544/130‑1 (MQ=255)                                                                        ggggCCTTCGGCGGCATTTAATCCCCTGACGGCCCTGCGGGGGGGCGCGCGGGCGGCCCGCGCCGGGGCGGTTGG‑CCGGGGCAAACGGC‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                        <  6:17258/133‑1 (MQ=255)                                                                        gcgGCCTTGGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGGGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                        <  3:243773/135‑1 (MQ=255)                                                                        ccgGCCTGCGGGGGCCTTGAATTCCCTGACTGCCGTGCCGGGGGGCGCGGGGGGGGCCCGCGCGGGCGCGCTTGT‑CGGGGGCAAACGGC‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAacac                                                        <  2:89888/134‑1 (MQ=255)                                                                           ggcTTCGGGTGTCTTGAATTCGCTGCCTGCCCTGCGGGCGGGGTCGCGGGCGGCCCGCGCCTGGGGGCTTTT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGt                                                     <  6:143749/133‑1 (MQ=255)                                                                           gcgttCGGCTGCCTTGATTTCCGTGACTCCCCTGCGGGGGTGCTCGCGGGCGGCCGGCGCGTGCGGGCTTGT‑CTGGGGCAACCGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGt                                                     <  6:81822/132‑1 (MQ=255)                                                                                cGGATGCCTTGAATTCCCTGACTGCCGTGCCGGCGTGGTCGCTGGGGGCCCGCGCGTGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACa                                                <  8:243971/135‑1 (MQ=255)                                                                                  ggtGCCTTGAATGCCCTGCCTGCCCGGCCGGGGTGCGGGCTGGGGGCCGGCGCGTGCGCGGTTGT‑CTGGGGCAAACGGG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt                                              <  6:248617/133‑1 (MQ=255)                                                                                  gcggCCTTGAATTCCAGGCCTGCCGGGCCGGGGTGCTGGCGGGCGGCCCGCGCCTGCGGGATTGT‑CAGGGGCAACCGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt                                              <  4:158177/132‑1 (MQ=255)                                                                                  gCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt                                              <  5:107579/135‑1 (MQ=255)                                                                                  gCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGT‑CTGGGGCAAACGGG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt                                              <  5:236247/135‑1 (MQ=255)                                                                                  gCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCGGGCGGCCCGCGCCTGCGCGCTTGT‑CAGGGGCAAACGGG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACAtt                                              <  3:114999/135‑1 (MQ=255)                                                                                         tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCGTGT‑GAGGGGCCAACGTG‑CCCCCGGCCCGGGGGATGGCCGTGGGGCGAGAGAAAGGAGAAACCCCGGGCACATTaaacctg                                       >  1:89888/1‑132 (MQ=255)                                                                                         tGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCAGCGCCTGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGt                                       >  1:191704/1‑135 (MQ=255)                                                                                                           tgccGGCGTGCTCGCTGGCGGCCCGCGCCGGCGCGGGTGT‑GAGGGGCCAACGTG‑CCCCCGGCCAGGGGGATGGCCGGGGGGCGTGCGGAAGGAGCAACACGGGGCACCTTACACAGTTGGAAGCGGCCCGGgcgc                     >  2:94244/1‑135 (MQ=255)                                                                                                                 cGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACCCGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGtt              >  8:134847/1‑136 (MQ=255)                                                                                                                   tGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGc            >  8:214443/1‑136 (MQ=255)                                                                                                                      tCGCTGGCGGCCCGCGCCTGCGCGGGTGT‑CAGGGGCAAACGTG‑CCCCCGGGCAGGGGTATGGCCGGGGGACGAGAGAAAGGAGGAACCCGGGGCACATTACCCAGTTGAAAGCGGCCCTGCCGCGTGCGTTGcgg          >  5:72467/1‑133 (MQ=255)                                                                                                                      tCGCTGGCGGCCCGCGCCTGCGCGCTTGT‑GAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGGGCGCCTAGAGAAAGGAGAAACACGGTGGACATTACACAGTTGAAAGCGGCCCTGCCGCGGGTGTTgggg          >  3:16590/1‑132 (MQ=255)                                                                                                                        gCTGGCGGCCCGCGCCGGCGCGCGTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGGTGAAAGCGGCCCTGCCGCGTGAGGTGCTGGCg       >  7:198559/1‑136 (MQ=255)                                                                                                                              ggcCCGCGCCGGCGCGCTTGG‑CCGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCggt  <  4:139218/135‑1 (MQ=255)                                                                                                                                        |                                                                                                                               AGAGGCCGCTACTAAGCGACCCGTCCGCCGCTAGAACCTTGATAGCCGGCTGCACCCTTCAGGGGTGTGGCGCGGCCTTCGGCTGCCTTGAATTCCCTGACTGCCCTGCCGGCGTGCTCGCTGGCGGCCCGCGCCTGCGCGCTTGT‑CAGGGGCAAACGTG‑CCCACGGCCAGGGGAATGGCCGTGCGACTAGAGAAAGGAGAAACACGGTGAACATTACACAGTTGAAAGCGGCCCTGCCGCGTGAGTTGCTGGCGTCGGT  >  KT2440/96345‑96604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑