| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,094,808 | A→C | 51.0% | L186R (CTG→CGG) | CJ019_01995 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,094,808 | 0 | A | C | 51.0% | ‑6.0 / 2.8 | 18 | L186R (CTG→CGG) | CJ019_01995 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/3); new base C (5/4); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTG‑GCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAA > KT2440/2094686‑2094938 | ccATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGGCCCGCGCGCCGG‑Gc > 1:170050/1‑135 (MQ=255) gCCCGGGCCAACAGTTTCTCAAGATACGTCATCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTTCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCCCGCCGGCGCCGGG‑GGGCCCGTTTTCAGGGccagg > 4:251312/1‑132 (MQ=255) tctattCATCCACCTGCGGCGCCGGCTGCTTGCTCAACTCCTGCACGCCGCGCGCGCCGGCCAGGCGCGCCGG‑GCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATgcagcag < 8:180593/132‑1 (MQ=255) tctTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCAG‑GCCGGGCTGGCCGTTTTGCAGGGCCAGTTGGCCGAGGTTGGTGAAGAACTCTATATgccgcagc > 4:57226/1‑135 (MQ=255) ctTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCCCGCCAG‑GCCGGGCTGGCCGTTTTTCAGGGCCGGTTTGCCGAGGTTGTTTAATAACTCTATATgcagcagca > 4:103628/1‑135 (MQ=255) ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTG‑GCCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGa > 8:143458/1‑136 (MQ=255) ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCACGCGCGCCGG‑GCCGGGGGGGGCTGTTTTTAGGGCCTGTTGGGCGCGGTTGTTGAAGAACTCTAAATGGAGCAGCACCCGGATATGgggg > 3:35134/1‑135 (MQ=255) cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTG‑GCCGGGCTGGCCGTTTTGCCGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCGATATGCAGCCGCACCAGGATATGGCGGagt > 8:134797/1‑134 (MQ=255) ccGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCGGGCGCGCCTG‑GCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGAttt < 3:8377/135‑1 (MQ=255) tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGCGCGCCGG‑GCCGGGCTGGGGGTTTTGCAGGGGCGGTTGGGCGCGGGTGTTGTAGAACTCTATATGCAGCAGCCCCAGGATATGGCGGCTTTACTTGa > 7:129518/1‑136 (MQ=255) tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGCGCGCCTG‑GCCCGGCGGGCCGTTTTTCAGGGCCGGGTGGCCGCGGGTGTTGAAGAACTCGATATGCAGCAGCACCCGGTAATGGCGGATTTACTTgc > 8:224612/1‑135 (MQ=255) tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCGG‑GCCGGGGGGGGCGGTTTTGAGGGCCGGTTGGGCGCGGTTGTTGTAGCACTCGTTATGCAGCAGCCCCAGGATATGGCGGCTTTTCttt > 2:6/1‑134 (MQ=255) cttcctcAAATCCTGCCCGCCCGGCTCGCGGCCCCCGCGCGCCGG‑CCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGAc < 3:103628/129‑1 (MQ=255) cgccgcGCGCGACGGCCAGGCGCGCCGG‑GCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGa < 4:37199/135‑1 (MQ=255) ccgcGGTCGCCGCCCAGGCGCGCGGG‑CCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACa < 5:211052/135‑1 (MQ=255) gccggCCCGGCGCGCCTG‑GCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag < 8:161878/133‑1 (MQ=255) ccggCCCGGCGCGCCGG‑GCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag < 3:140142/133‑1 (MQ=255) cggCCCGGGGCGCCGG‑CCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaagaa < 7:75528/135‑1 (MQ=255) | CCATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGCGCGCCTG‑GCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAGAA > KT2440/2094686‑2094938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |