| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,387,001 | A→C | 52.2% | intergenic (+70/+36) | CJ019_02252 → / ← dacC_1 | hypothetical protein/D‑alanyl‑D‑alanine carboxypeptidase DacC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,387,001 | 0 | A | C | 52.2% | ‑1.1 / 10.6 | 15 | intergenic (+70/+36) | CJ019_02252/dacC_1 | hypothetical protein/D‑alanyl‑D‑alanine carboxypeptidase DacC |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base C (3/5); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-01 | |||||||||||
CGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCG‑CAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAGCACCTGCGAGGCACCCCACGGTTTCGGGTCGT > KT2440/2386871‑2387134 | cGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGGGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCGTTCGGGCCGGAAAACCGCGCCGGCAGCGCCcgcg > 7:95207/1‑136 (MQ=255) tataTGCGGCCAAACCGGCGGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGGGCGCGCGGTCGGCGGGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCG‑CAAGCgg < 4:159530/135‑1 (MQ=255) atatGCGGCCAAACCGGCGGGGCGGGAGCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGGCGGCGTGGGGGCCGCTTTGCGGCCGTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCG‑CAAGCggg < 2:47397/135‑1 (MQ=39) aCAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCATTTCGGGCCCTTGGGGGCGCAAAGCCCCGCCGGCCACGCCCCCGCCAACCGGGCCCGGg‑gtgtg > 2:78090/1‑135 (MQ=255) gggAGCCGGAATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCGTTTAGGCCCCTTGGGGGCCCCAAGCCCCCCCAGCCTCGCCCCCC‑CCCCCCGGCCCCGGTGGTTGGCAGGTGGCGTCACTTGGccccggg > 6:86957/1‑129 (MQ=255) ccGGGCGCGCGGGCGGCGGGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCG‑CAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCg < 3:21988/135‑1 (MQ=255) gggcgcggGGGGGGCGGGGGGGCGGGTTGGCGGCCCTTTCGGGACGAAAAGCCGCTCCGGCATCGACCGGG‑CATGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGc < 1:78090/133‑1 (MQ=255) ggcgggtttGCGGCCGTTGCGGGCGGCAACGCCGCGCCGGCATCGCCCGCG‑CACGGGGGCGCGGGTGTGGGGCTGGGGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCaa < 2:89416/129‑1 (MQ=255) gggACGCAACGCCGCGCCGGCAGCGCCCGCG‑CAAGCGGGCCCGGGGGTGTGGCAGGTGGCGGCAGTTGGCCGCGGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAGCACCTGCg < 5:86957/135‑1 (MQ=255) ggACGCAAAGCCGCTCCTGCATCGACCGCG‑CAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCGGGCGATACAGGTCAAGCAACACCTGATCCAGCACCTGCGAg > 8:274929/1‑136 (MQ=255) cAAGGCCGCTCCGGCTGCGCCGGCG‑CAAGCGGGCCCGGGGGTGTGGCCGGTGGCGTCAGTTGGCCGCGGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAGCACCTGCGAGGCAc < 1:168460/135‑1 (MQ=255) cTGCAGCGCCCGCG‑CAAGCGGGCCCGGGTGTGTGGCAGGGGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAGCACCTGCGAGGCACCCCACGGTTTc < 2:111438/135‑1 (MQ=255) tCGCCCGCG‑CAAGCGGGCCCGGGGGTGTGGCAGGGGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAGCACCTGCGAGGCACCCCACGGTTTCGGGTc < 6:227571/135‑1 (MQ=255) cGACCGCG‑CAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAGCACCTGCGAGGCACCCCCCGGTTTCGGGTCGt > 8:111230/1‑136 (MQ=255) gACCGCG‑CAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCCGCACCTGCGAGGCCCCCCCCGGTGTCGGGTCGt > 2:112233/1‑135 (MQ=255) | CGCCGGCGTATATGCGGCCAAACAGGCCGGGCGGGACCGGGTGGAGGGGGAGCCGGTATAAAACCGTGCGCGCGGTCGGCGTGGGGGCCGCTTTGCGGCCCTTTCGGGACGCAAAGCCGCTCCTGCATCGACCGCG‑CAAGCGGGCCCGGGTGTGTGGCAGGTGGCGTCAGTTGGCCGCTGTACTGCCAGCCAGCTTCGGCTGGCGATACAGGTCAAGCAACACCTGATCCAGCACCTGCGAGGCACCCCACGGTTTCGGGTCGT > KT2440/2386871‑2387134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |