| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 288,790 | C→G | 25.4% | intergenic (+90/+52) | CJ019_00262 → / ← CJ019_00263 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 288,790 | 0 | C | G | 25.4% | 55.2 / 4.0 | 34 | intergenic (+90/+52) | CJ019_00262/CJ019_00263 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (18/7); new base G (7/2); total (25/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCGCTCCCACA‑GGTACTCGCAAGCTCCAGGGTGCGCGCGGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCTTCGAACACCCGCTTGCCTTCCTCGGCC > KT2440/288662‑288920 | tttGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCGCTCCCACA‑GGTACTCGCAAGCTCCAGGGTGCGCGCGGG‑CTCTGTgg > 7:217455/1‑136 (MQ=255) gAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCGCTCCCCCA‑GGGACACCCCACCTACACGGTGCGCGCGGG‑CTCTGGGGGGGCg > 7:8890/1‑136 (MQ=37) gAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCCCTACCCCA‑GGTACTCGCAAGCTCCAGGGTGCGCGCCGG‑GTCTGTGGGAGc > 5:133924/1‑135 (MQ=39) aGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCGCTCCCACA‑GGTACTCTCAAGCTCCAGGGTGCGCGCGGG‑CTCTGTGGGAGCgg > 5:58618/1‑135 (MQ=255) gACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCCCGCCCCCA‑CGTACTCGCCACCGCCCGGGGGCGCGCGGG‑GGGGGGGGGAGCgggggt > 3:191642/1‑132 (MQ=25) gACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGACTGCTTTGCAGCCCTTCGCCGGCACGCCCGCACCCCCA‑CGGACACCCAAGCGCCAGGGTGCGCCCGGG‑GGCTGGGGGCGCgggggt > 6:217407/1‑132 (MQ=255) aCCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCCCACCCACA‑GGGACTCGCCAGCTCCAGGGTGGCCGCGGG‑GTCTGTGGGAGCGGGCATg > 5:252229/1‑135 (MQ=38) ccACGCTGGGAGGGGGGGGGGCGATTTTGGGGCGGCTTGCCCCCCCTTCGCCGGCACGCCCGCTCCCCCA‑GGACCTCGCAAGCTCCAGGGGGCGCGCGGG‑CTCTGGGGGAGCGGGCTTGCCCGCGAAGGgccgcc < 6:133924/135‑1 (MQ=255) tgtgATGCGGCGGGTCGATATTGGGGCTGCTTTGCCGCCCTTCGCCGGCACGCCCCCTCCCACA‑AGGACGCCCCACCCCCCCGGGGCCCGCCGG‑GTCGGGGGGCGCGGGCGTGCCCGCCCCCGGCCGCCaccccgc > 8:144309/1‑130 (MQ=255) tgtgATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGGCCCCGCCCCCACGGTA‑GCGCCAACACCCCGGTGGGCGCGGG‑GGGTGGGGGAGCGGGGGTGGCCGCGCCCGGCCGCCCAggcg > 5:275836/1‑132 (MQ=255) ggTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCGCTCCCACA‑GGTACTCGCAAGCGCCAGGGTGCGCGCGGG‑CGCTGTGGGCGCGGGCGTGCCCGCGCCGGGCCGCCCCGCGGCCCCAGCTTtct > 1:45434/1‑135 (MQ=37) ggTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCGCGCCCACA‑GGTACTCGCAAGCTCCAGGGTGCGCGCGGG‑CTCGTTGGGAGCGGGCTTGGCCGCGCAGGGCCGCCCAGCCGCCCCCGAatta > 4:76224/1‑133 (MQ=37) ggTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCCCGCCCCCC‑GGGTCACCCCAGCTCCCGGGTGCGCGCGGG‑CGGGGTGGGGGCGGGCGTGCCCGCGCAGGGCCCCCCACCCGCCCCCGAgtttg > 5:231233/1‑133 (MQ=255) cGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCGCTCCCACA‑GGTACTCGCAAGCTCCAGGGTGCGCGCGGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAg > 4:166121/1‑135 (MQ=255) ttGCAGCCCTTCGCCGGCACGCCCGCTCCCCCA‑GGTTCTGGCAAGCTCCAGGGTGGGCGCGGG‑GTCTGTGGGAGCGGGGTTGCCCGCGCCGGGCCGCCCAGCGGCCCCCGCTTTCTCAGCTGGGACGCGTGGGCa > 4:35606/1‑135 (MQ=255) cTTCGCCGCCCCGCCCGCAACAACC‑GGACCTCGCATGCGCCAGGG‑GGGCGGGGGCCACTGGCGCAGGTGGCTTGCCCGCGATGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGACAcgtc < 4:76585/135‑3 (MQ=255) cGGCACGCCCGCTCCAACA‑GTTACGCGCACGCTCCAGGGTGCGCGCGGG‑CTCTGTGGGAGGGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGa < 3:76224/135‑1 (MQ=39) gCTCCCACA‑GGTACTCGCAAGCTCCAGGGTGCGCGGGGG‑CGCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCa < 4:33769/135‑1 (MQ=255) ccACT‑GGTACTCCCACGCTCCTGGGTGCGCGCGGG‑CGCTGGGGTCGCGGGCTTGCCCGCGACGGGCCGCCAAGCGGCCCAAGATTTCTCAGCTGGACCGCTTGGCCATAAACGGCGGCAGATACAACCCCAGATAc < 7:62863/136‑1 (MQ=255) ggTACTCGCAAGCTCCAGGGTGCGCGCGGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCCGCTGGCACGCGTGGCCAGAAACGGCGGCAGATCAAACCCCACAGACGCTTc > 8:248629/1‑136 (MQ=38) ggTACTCGCAAGCTCCAGGGTGCGCGCGGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGCACGCTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCtt > 2:5976/1‑135 (MQ=255) gtccTCGCATGCTCCGGGGGGGGGGGGGG‑GGGTGTGGGAGGGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCTTc < 1:215065/132‑1 (MQ=37) cTCGCAAGCTCCAGGGTGCGCGCGGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCTTCGaa > 4:91449/1‑135 (MQ=255) cGCAAGCTCCAGGGTGCGCGCGGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCTTCGAaca > 3:136243/1‑135 (MQ=255) gcccggggGCGGGGGGG‑CTCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCTTCGAACACCCGCtt < 3:218513/131‑1 (MQ=38) cAGGGTGCGCGCGGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACCGCGGCAGATACCACCCCAGATACGCTTCGAACACCCGCATGc > 6:223552/1‑135 (MQ=255) ggggggcgcgGG‑GCGTGGGGGGGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCTTCGAACACCCGCTTGCCtt < 3:35606/130‑1 (MQ=38) gTGGGCGCGGG‑CTATGTGTGAGCTGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGTAACGGTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCTTCGAACACCCGCTTGCCtt < 5:138594/134‑1 (MQ=39) gcgGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGCTTTCTCAGCTGGAACGCTTGGCCAGAACCGGCGGCAGCTACAACCCCAGATACGCTTCGAACACCCGCTTGCCTTACTCGGc > 8:90436/1‑135 (MQ=255) gcgGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCCGATACGCTTCGCACACCCGCTTGCCTTCCTCGGc > 6:79819/1‑135 (MQ=255) gcgGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCTTCGAACACCCGCTTGCCTTCCTCGGcc > 7:198473/1‑136 (MQ=255) gcgGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATAAAACCCCAGATACGCTTCGAACACCCCCTTGCCTTCCTCGGc > 6:230801/1‑135 (MQ=255) gcgGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGACCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCACCAACTCTTCCAACCCCCGCTTGCCTTCATCgg > 6:138594/1‑134 (MQ=38) gcgGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAACGGCCCCCGAATTTTCAGCTGGAACGCTTGGCCACAAACGGCCGCAGAATCCACCCCAGATACGCATCGAACACCCCCTTGCCTTCCGAGGcc > 7:160120/1‑136 (MQ=37) | TTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCGCTCCCACA‑GGTACTCGCAAGCTCCAGGGTGCGCGCGGG‑CTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCAGATACAACCCCAGATACGCTTCGAACACCCGCTTGCCTTCCTCGGCC > KT2440/288662‑288920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |