| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 434,368 | 0 | T | G | 58.8% | 3.6 / 14.1 | 18 | W67G (TGG→GGG) | bioF | 8‑amino‑7‑oxononanoate synthase |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base G (1/9); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.50e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCC > KT2440/434252‑434481 | aaGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGGAGCCATGAAGACCTGGGCCTGGCCAACCACCCCGAGGTGATAGCCCCCAGGCAGGCCCGCGCCGCGCGCGGGGGTGTggggggggggg > 8:205750/1‑135 (MQ=255) gggggtCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGT‑‑GGGGGGGGGCCTCGCACCTGGTa < 5:266531/131‑1 (MQ=255) gcctcgggTTGGCGGCCTTGTCCCCCAATGACTACCGGCGCCTGGCCAACCCCCCCCTGGGCACCCCCGCCCGGCCGGCCGGGCCCGCGCGCGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCcaca < 6:61941/129‑1 (MQ=255) gcgggCCTTCTGCCGCAATGCCTACCTGGCCCGGGCCAACCCCCCCGAGGGGCTCGCCGCCTGGCCGGCCGGCGCCGAGCG‑GGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc < 8:243855/132‑1 (MQ=255) ggCCTTCTGCAGCAATGACAACCATGGACTGGACAACAACCCCGAGATGATCGCCGCCTGGCAGGGCGGCGCCGAGCGAAGGGGTTTGTTGGGGGGGGCAGAGCACGTGGTAGATTGCCACACCACCCCccacca > 4:75912/1‑135 (MQ=255) cggggCCGGCCCAACCCCCCCGAGGTGTGCCCCGCCGGGCAGGCCGGCGCCGCGGGCGGGGGTGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTgg < 8:6070/131‑1 (MQ=255) ggCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCGCGCACCTGGTAGTTGGCCACAGCACCCCGCACCAACAGGTTGAAGAGGCACTGGCTGaa > 6:9044/1‑135 (MQ=255) tGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCGGGTAGTTGGCCACAGCCCCCCGCACCATCAGGTTGAAGAGGCCCTGGCTGAAATCa > 3:134555/1‑135 (MQ=255) gCCAACCCCCCCGGGGGGCCCGCCGCCGGGCCGGCCGGCGCCGCGCGCGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCAcc < 5:108195/135‑1 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCCCAGCACCCCGCACCATCAGGTTGAAGAGGCCCTGGCTGAACTCACCGgc > 4:282305/1‑135 (MQ=255) aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACCGCACCCCGCACCATCAGGTGGAAGAGGCACGGGCGGAACTCACCGgc > 7:136284/1‑135 (MQ=255) cccccGCGGTTAGCGCCGCCTGGCAGGCCGGCGCCGCGGGCGGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgccgcc < 3:163950/134‑1 (MQ=255) gggATCGCGGCGTGGCAGGGGGGCGCGGCCGGCGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGcc < 7:133098/133‑1 (MQ=255) ccgccTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCCCCCCGCCCCATCAGGTGGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCtggtgt > 3:204430/1‑135 (MQ=255) ccgggccggccGGCGCCGCGCGGGGGGGGGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTtct < 6:153011/132‑1 (MQ=255) gggCTGGCCGGGGCCGCGCGGGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTcc < 4:170360/134‑1 (MQ=255) gggCAGGCCGGGGCCGCGGGGTGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTcc < 8:42941/134‑1 (MQ=255) gggCAGGCCGGCGCCGTGCGCGGGGGGGGGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTcc < 6:241623/134‑1 (MQ=255) | AAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGTGTGGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCCGCGCGCCCTGTTGTTCTCC > KT2440/434252‑434481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |