Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,094,8080AC51.7% ‑3.5 / 10.2 30L186R (CTG→CGG) CJ019_01995hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (9/5);  new base C (12/3);  total (21/9)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGC‑GCGCC‑TGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  KT2440/2094688‑2094936
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aTGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGCAGGCCCGGC‑GCGCC‑GGGGCg                                                                                                                    >  7:49827/1‑135 (MQ=255)
 ggCGGTCCAGTCCCGGGGCATGGGCCCGGGCCAACAGTTTCTCAAGATACGTCCGCTGCTCTTCATACCCCTTCGGCTCCGGCTGCTTGCTCAAATACTGCACGCCGCGCTCGACGGCCCGGC‑GCgcc‑gggccgg                                                                                                                   <  8:49827/134‑1 (MQ=255)
     gTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCCGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGGCCCGC‑GCCCC‑Ggggcgggcgg                                                                                                               >  6:36718/1‑133 (MQ=255)
     gTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGC‑GCGcc‑gggccgggggg                                                                                                               >  8:257514/1‑132 (MQ=255)
         aGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGC‑GCGCC‑cgggcgggcggggcg                                                                                                           >  7:114798/1‑132 (MQ=255)
                   cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGCAGGCCCCGC‑GCGCC‑GGGCCGGGCGGGGCGTTTTGGAggg                                                                                                 >  2:156974/1‑135 (MQ=255)
                   cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGC‑GCGCCGGGGCCGGGGGGGCGGTTTTgaggg                                                                                                  >  3:164835/1‑131 (MQ=255)
                   cATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTAGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGGCACGCGGGGCC‑GGGCCGGGGGGGCGGTTTTGCTgg                                                                                                  >  2:131407/1‑135 (MQ=255)
                              gCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGC‑GCGCC‑TGGGCGGGGGGGCCGTTTTGCAGGGGCGGGTGGCCg                                                                                      >  4:56357/1‑135 (MQ=255)
                                          tcAAGATCCGGCAGGTGCTCTCCATCCCCCTGCGGGCCCGGCTGCTGGCGCAAATCCTCCCCGCCGGGCGCGCCGGCCAGGC‑GCGCG‑GGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTgaa                                                                           <  4:113981/134‑1 (MQ=255)
                                           cAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGC‑GCGCC‑TGGCCGGGCGGGCCTTTTTGCAGGGCCGGGTGGGCGAGGGTGTTgaaga                                                                         >  6:96824/1‑135 (MQ=255)
                                                   gTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGC‑GCGCC‑TGGCCGGGCTGGCCGTTTTTCAGGGCCGGTTGGCCGCGGTTGTTGAAGAACTCtata                                                                 >  3:34343/1‑135 (MQ=255)
                                                    tCAGCGGCTCTTCAACCCCCTCCGGCTCCGGCGGCTGGCGCAAATCCTGCACGCCGCGGTCGACGGCCAGGC‑GCGCC‑GGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTatat                                                                <  4:78343/135‑1 (MQ=255)
                                                           ctctTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGC‑GCCCC‑GGGCCGGGGTGGGCGGTTTTCAGGGGCGGTTTGGCGAGGGTTTTGAAGAACTCTAAATgatttct                                                         >  3:26591/1‑129 (MQ=255)
                                                            tctTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGC‑GCGCC‑GGGGCCGGCGGGCCGTTTTTGAGGGCCGGTTTGGCGCGGTTGTTGTAGAAAACAATATtaagcagc                                                        >  7:249644/1‑135 (MQ=255)
                                                                   acacCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGC‑GCGCC‑TGGCCGGGCAGGCCTGTTTGCAGGGGCGGTTGGCCGCGGGTGTTGAAGACCTCTATATGGAGCAGCACCa                                                    >  2:196788/1‑132 (MQ=255)
                                                                   acacCTTCGGCTCCGGCTGCTTGCGCAAATAATGCACGCCGCGCTCGACGGCCCGGC‑GCGAC‑TGGGCGCGCTGGACGTTTTGCAGGGCCGTTGGGCGGTGGGTGTTGAAGTAATGTCTATTCAGCAGCACCAGGa                                                 >  8:216284/1‑135 (MQ=255)
                                                                           ggCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCGGC‑GCGCC‑TGGCCGGGCGGGCCGTTTTGGAGGGCCGGTTGGGCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCCGGATATGgggg                                         >  3:189123/1‑135 (MQ=255)
                                                                             cTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCCCGC‑GCGCC‑TGGCCGGGCTGGCCTTTTTGCACGGCCGGTTGGCCGCGGTTGTTGAAGAACGCTATATGCAGCAGCACCAGGAGATGGCGGag                                       >  1:47851/1‑134 (MQ=255)
                                                                                    tgctTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGC‑GCGCC‑TGGGCGGGCTGGCCGTTTTTGAGGGGCGGGTGGGCGAGGTTGTTGAAGAAATCTATATTGAGCACCCCCAGGAGTTTGCGGAttttcatg                                >  8:18551/1‑130 (MQ=255)
                                                                                      ctggcgcAAATACTCCCCGCCCGGCTCGACGCCCAGGC‑GCGCC‑GGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGAc                              <  8:66369/132‑1 (MQ=255)
                                                                                          cgcAAATACTGCACGCCGCGCTCGAAGGCCAGGC‑GCGCC‑TGGCCGGGCTGGCCTTTTTGCAGGGCCGGGTGGCCGGGGTTGTTGAAGAAATCTATATGCCCCCGCACCAGGATATGGCGGATTTCCTTGccccca                          >  6:289665/1‑133 (MQ=255)
                                                                                                        gccgcGCTCGAAGGCCAGGC‑GCGCC‑TGGCCGGGCTGGCCGTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGTAGAACTCTATATGCAGCAGCCCCAGGATATGGCGGATTTCCTTGACCCAATGGTTGGCCTCg              >  2:235917/1‑133 (MQ=255)
                                                                                                        gccgcGCTCGAAGGCCAGGC‑GCGCC‑TGGCCGGGCTGGCCGGTTTGCCGGGGCTGTTGGGCGAGGTTGTTGAAGAACTCTATATTCAGCAGCCCCAGGATATGGCGGCTTTCCTTGACCCAATGCTTGGCcgcg              >  2:232145/1‑133 (MQ=255)
                                                                                                        gccgcGCTCGAAGGCCAGGC‑GCGCC‑GGGCCGGGCTGGCCGTTTTTCAGGGCCGGGTGGGCGAGGTTGTTGAAGAACTCTATATGCAGCAGCCCCAGGATATGGCGGGTTTCCTTGACCCAATGCTTGGCCTCGAc            >  1:19453/1‑135 (MQ=255)
                                                                                                          cgcgcgcgACGGCCCGGC‑GCGCC‑GGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGAccg          <  7:23226/135‑3 (MQ=255)
                                                                                                               gcGAGGGCCCGGC‑GCGCG‑GGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGc     <  6:95901/134‑1 (MQ=255)
                                                                                                                 gccggCCGGGC‑GCGCG‑GGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaa   <  3:222618/132‑1 (MQ=255)
                                                                                                                  acggCCAGGC‑GCGCC‑GGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  4:183210/133‑1 (MQ=255)
                                                                                                                  acgcccAGGC‑GCGCC‑GGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCaag  <  2:246526/131‑1 (MQ=255)
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ATGCGGTCCAGTACCTGGGCATTGGCCCGGGCCAACAGTTTCTCAAGATACGTCAGCTGCTCTTCATACACCTTCGGCTCCGGCTGCTTGCGCAAATACTGCACGCCGCGCTCGAAGGCCAGGC‑GCGCC‑TGGCCGGGCTGGCCTTTTTGCAGGGCCTGTTGGCCGAGGTTGTTGAAGAACTCTATATGCAGCAGCACCAGGATATGGCGGATTTCCTTGACCCAATGCTTGGCCTCGACAGCGGGCAAG  >  KT2440/2094688‑2094936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑