Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,322,1790TG54.5% 6.5 / 18.0 22V237G (GTG→GGG) fadB_1Fatty acid oxidation complex subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (3/7);  new base G (0/12);  total (3/19)
Fisher's exact test for biased strand distribution p-value = 7.79e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.97e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GGCAATCGCATGTTGCGCAGCTATGTGGGCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGTGGTGGGCATCGACCTGGAGTGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCTCGGCCGCCTTGGGCAGAAGACCGGGCAGGGCTATTACCGC  >  KT2440/2322046‑2322309
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ggCAATCGCATGTTGCGCAGCTATGTGGGCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACgtggtg                                                                                                                                   >  5:474/1‑135 (MQ=255)
  cAATCGCATGTTTCGCAGCTATGTGGGCGAAGCGCGCTAGTTGCTGCTTGAAGGGGCGGTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGtggtgg                                                                                                                                  <  4:1031/134‑1 (MQ=255)
  cAATCGCATGTTGCGCAGCTATGTGGGCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGtggtgg                                                                                                                                  <  7:112896/134‑1 (MQ=255)
  cAATCGCATGTTGCGCAGCTATGTGGGCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGTGGTggg                                                                                                                                 <  4:79293/135‑1 (MQ=255)
  cAATCGCATGTTGCGCAGCTATGTGGGCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGTGGTggg                                                                                                                                 <  6:243911/135‑1 (MQ=255)
  cAATCGCATGTTGCGCAGCTATGTGGGCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGTGGTggg                                                                                                                                 <  4:41705/135‑1 (MQ=255)
  cAATCGCATGTTGCGCAGCTATGTGGGCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCACCATGGGGCCGTTCCGCATGTACGACGtggtgg                                                                                                                                  <  8:218772/134‑1 (MQ=255)
      cGCATGTTGCGCAGCTATGTGGGCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGTGGTGGGCATc                                                                                                                             <  2:139036/135‑1 (MQ=255)
                          gggCGCAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGTGGTGGGCATCGACCTGGAGTGgcgcggggg                                                                                                         >  1:142650/1‑131 (MQ=255)
                          gggCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGTGGTGGGCATCGCCCTGGAGTGGCGCGgccc                                                                                                         >  4:73494/1‑134 (MQ=255)
                            gCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGTGGTGGGCATCGACCTGGAGTGgcgcgggcccg                                                                                                       >  3:133092/1‑129 (MQ=255)
                                    cgcAAGTTGCTGCTTGAAGGGGCGTGGCCACATCAGGTGGATGCGGTACTGCAGCAGTGCGGCTGCGCCATGGGGCCGTTCCGCATGTACGACGTGGTGGGCATCGACCTGGAGTGGCGCGCCCGGCAGTTGGcc                                                                                               <  2:166323/135‑1 (MQ=255)
                                     gcAAGTTGCTGCTTGAAGGGGCGGTGCCACATCAGGGGGATGCGGTACTGCAGCAGTTCGGCTTCGCCAGGGGGCCGTTCCGCATGTACGACGTGGTGGGCATCGACCTGGAGTGGCGCGCCCGGCAGTTGGCCg                                                                                              <  7:100234/135‑1 (MQ=255)
                                        aggtgcggcGTGAAGGGGCGGTGCCACATCAGGTGGAGGCGGTACTGCCGCAGTTCGGCTGCCCCATGGGGCCGTTCCGCATGTACGACGTGGGGGGCATCGACCTGGAGTGGCGCGCCCGGCAGTTGGCCGGGc                                                                                           <  6:223735/132‑1 (MQ=255)
                                                            ttGCCACATCAGGTGGAGGCGGTACTGCAGCAGTGCGGCTGCGCCATGGGGCCGTGCCGCATGTACGACGGGGGGGGCATCGACCTGGAGTGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTg                                                                       <  8:16530/135‑1 (MQ=255)
                                                                 acaTCAGGTGGAGGCGGTACTGCAGCAGTGCGGCTTCGCCAGGGGGCCGTTCCGCATGTACGACGTGGGGGGCATCGACCGGGCGGGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCa                                                                  <  8:114981/135‑1 (MQ=255)
                                                                           gaggCGGAACTGCAGCAGTGCGGCTTCGCCATGGGGCCGTGCCGCATGTACGACGGGGTGGGCATCGACCTGGAGTGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAAt                                                        <  8:250890/132‑1 (MQ=255)
                                                                           gaggCGGAACGGCAGCTGGTCGGCTGCGCCAGGGGGCCGTGCCGCATGTACGACGGGGGGGGAAGCGCCCTGGCGGGGCGCGCCCGGCAGTGGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAAt                                                        <  6:158577/132‑1 (MQ=255)
                                                                           gacgCGGTACTCCAGCAGTGCGGCTGCGCCTGGGGGCCGTGCCGCATGTACGACGGGGGGGGCAGCGCCCTGGAGTGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAAt                                                        <  1:196952/132‑1 (MQ=255)
                                                                                         ctagTGCGGGTTCGCCTTGGGGCCCTACGTCATTGACGGGGTGGGGGGAAGCGACGGGGTGGGTCGCGCCCGGCCGGGGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCt                                          <  6:184978/133‑1 (MQ=255)
                                                                                              tCGGCTGCGCCAGGGGGCCGTGCCGCATGTACGCCGGGGGGGGCAGCGACCTGGCGTGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCTCGgcc                                     <  3:53686/135‑1 (MQ=255)
                                                                                                          tGGGGCCGTTCCGCATGTACGCCGTGGGGGGCATCGACCTGGAGGGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCTCGGCCGCCTTGGGCaga                         <  4:52561/135‑1 (MQ=255)
                                                                                                          tGGGGCCGTGCCGCAGGTACGCCGGGGGGGGCAGCGAGGGGGCGTGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCGCGGCCGCCTTGGGCaga                         <  3:73494/135‑1 (MQ=255)
                                                                                                                cGTTCCGAATGTACGCCGTGGGGGGCAGCGACCTGGAGTGGCGGGCCCGCCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCTCGGCCGCCTTGGGCAGAAGACCg                   <  1:19403/135‑1 (MQ=255)
                                                                                                                     cgaaTGTACGCCGTGGGGGGCAGCGACGGGGAGTGGCGCGCCCGGCTGTGGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCTCGGCCGCCTTGGGCAGAAGACCGGGCa               <  6:146048/131‑1 (MQ=255)
                                                                                                                        ttgcccgacgTGGGGGGCCGCGCCCGGGCGGGGCGCGCCGGGCAGGGGGCCGGGCAAGGCAGGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCTCGGCCGCCTTGGGCAGAAGACCGGGCAGGGc           <  1:40010/130‑1 (MQ=255)
                                                                                                                               tggtggggGGCAGGGACCTGGCGTGGCGCGCCGGTCGTTGGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCTCGGCCGCCTTGGGCAGAAGACCGGGCAGGGCTATTAcc    <  7:94766/133‑1 (MQ=255)
                                                                                                                                 ggggtggGCATCGACCTGGAGTGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCTCGGCCGCCTTGGGCAGAAGACCGGGCAGGGCTATTACCGc  <  4:133092/133‑1 (MQ=255)
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GGCAATCGCATGTTGCGCAGCTATGTGGGCGAAGCGCGCAAGTTGCTGCTTGAAGGGGCGTTGCCACATCAGGTGGATGCGGTACTGCAGCAGTTCGGCTTCGCCATGGGGCCGTTCCGCATGTACGACGTGGTGGGCATCGACCTGGAGTGGCGCGCCCGGCAGTTGGCCGGGCAAGGCATGCACGACCCGCTGGTGCAGGTCGATAATGCGCTGTGCGAGCTCGGCCGCCTTGGGCAGAAGACCGGGCAGGGCTATTACCGC  >  KT2440/2322046‑2322309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑