Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,138,3670TG31.7% 65.0 / 10.2 41F240V (TTT→GTT) rbsC_2Ribose import permease protein RbsC
Reads supporting (aligned to +/- strand):  ref base T (3/25);  new base G (13/0);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 3.18e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG  >  KT2440/3138237‑3138499
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cacGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGtgttt                                                                                                                                  >  1:94749/1‑134 (MQ=255)
 acGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTGTTgg                                                                                                                                 >  4:36868/1‑134 (MQ=255)
       ttCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                           <  1:241768/135‑1 (MQ=255)
       ttCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                           <  2:158143/135‑1 (MQ=255)
       ttCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGGCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                           <  7:206399/135‑1 (MQ=255)
       ttCGGCCTGCGCCTGTACGCCATCGGAGAATACCCGGCGGCGGCTAATGCCGCGGGCCTGTCGGGGCGTGGCTACGTGTTCGCCAGCTACCTGATCCGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcg                                                                                                                           <  7:218407/135‑1 (MQ=255)
        tCGGCCTGCGTATGTACGCCCTCGGAGAATACCCGGAGTCGGATAATGCCGCTTGCCTGTCGGTGCGTTGCTACGTGTTCGCCAGCTACCTTATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTcggcgg                                                                                                                          <  6:39997/135‑1 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTTTTTTGCGGGCt                                                                                                                       >  8:276004/1‑135 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTgggggggcg                                                                                                                       >  4:220661/1‑132 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTgggcgggcg                                                                                                                       >  7:220391/1‑132 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTTGGCGGccg                                                                                                                       >  3:117237/1‑134 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTCGGCGGCCt                                                                                                                       >  8:146234/1‑135 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGTTgggggggcg                                                                                                                       >  7:267474/1‑132 (MQ=255)
           gCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCCAATGCCGCTGGCCAGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGGTGGGGGGccg                                                                                                                       >  6:221540/1‑134 (MQ=255)
           gCCTGCGTCTGTACGCCATCCGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCAGGCCGCGGTGTGGTCCGCGcccg                                                                                                                       >  1:20723/1‑134 (MQ=255)
                cGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGt                                                                                                                    >  4:210964/1‑133 (MQ=255)
                  tCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAg                                                                                                                <  5:230032/135‑1 (MQ=255)
                       aCGCCATCGGAGAATACCCGGCGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCgg                                                                                                             <  7:288930/133‑1 (MQ=255)
                       aCGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCgg                                                                                                             <  3:224611/133‑1 (MQ=255)
                                      aCCCGGCGTCGGCTACTGCGGCTGGGCTGTCGGTGCGTGGCTAGGTGTGCGCCAGCTACCTGAGCAGCGGTGTGTGCGGCAGGCTGGCCTCGTTTTGTTAGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCg                                                                                            <  2:20723/135‑1 (MQ=255)
                                        ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGc                                                                                          <  7:162762/135‑1 (MQ=255)
                                        ccGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGGTTGTTGGGGGGGCGGGTTGAGCGGCCGCCCCCCCGGTggcggc                                                                                          >  3:97110/1‑135 (MQ=255)
                                             ggcggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAt                                                                                     <  8:163421/135‑1 (MQ=255)
                                                ggcTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGAtgct                                                                                  <  2:271543/135‑1 (MQ=255)
                                                  cTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTTGTTCAGCGGCACCACCACCGGTTACGGCGAGAagctgc                                                                                >  8:157858/1‑135 (MQ=255)
                                                     aTGCCGCTGGCCTGTCGGTGCGTGGCGACGTGTTCGCCAGCTACGTGATCAGCGGTCTGTGCGGCAGCGTGGCCGCGTTTTGTTCTGCGGCCTGGTTCAGCGGCAGCACGACCGGGTCCGGCGAGATGCTGCTGt                                                                             <  7:43195/135‑1 (MQ=255)
                                                               ccTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGc                                                                   <  4:81478/135‑1 (MQ=255)
                                                                    cGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCAt                                                                <  4:33509/133‑1 (MQ=255)
                                                                              cTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTgg                                                    <  4:128908/135‑1 (MQ=255)
                                                                              cTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGAACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTg                                                     <  7:138177/134‑1 (MQ=255)
                                                                                aCGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGGGGCCGGGTTCAGCGGCCGAACCACCCGTTCCGGCGCGAATGAGGTGTCCGTGGTGGCCAACGCGTTTGTTtggg                                                  >  8:138177/1‑135 (MQ=255)
                                                                                    gtTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTAGGCGGGCGGGTTAATCGGCCGCACCCCCCGGTGCGGCGCGCTGCTGGTGTGCGTGGTGGGGCTCGCTGTTTTTGGGGTggt                                              >  3:89011/1‑133 (MQ=255)
                                                                                          cAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTtctc                                        <  7:249382/135‑1 (MQ=255)
                                                                                                ccTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGGTTTGTTGGGCGGCCGGGGTGAGCGGCCGCCCCACCCGGTCCGGCGAGCTGCTGGGGTGGGGGGTGGGCCTCGCGGTTGTTTGGGGGGTCTTCTgcccccc                                  >  4:22801/1‑131 (MQ=255)
                                                                                                       aGCGGGCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTGCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGGGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAg                           <  3:57133/135‑1 (MQ=255)
                                                                                                            tCTGTGCGGCAGCCTGGCCGCGGTTTTTTAGGCGGCCTGGTTCAGCGGCAGCCCCACCGGGTACTGCGAGATGCTTCTGTACGTGGTGGGCAACGCGTTTCGTGGGGTTGACTTCGCCCAGCGGATGCAGCCGa                       >  8:151279/1‑134 (MQ=255)
                                                                                                                    gCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggcg              <  5:65177/135‑1 (MQ=255)
                                                                                                                    gCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGGTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATcggcg              <  1:77019/135‑1 (MQ=255)
                                                                                                                       gCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCa           <  4:117237/135‑1 (MQ=255)
                                                                                                                             ccGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACgctgc     <  7:177385/135‑1 (MQ=255)
                                                                                                                                cgTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTgg  <  6:205610/135‑1 (MQ=255)
                                                                                                                                cgTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTgg  <  3:112523/135‑1 (MQ=255)
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CACGCCATTCGGCCTGCGTCTGTACGCCATCGGAGAATACCCGGAGGCGGCTAATGCCGCTGGCCTGTCGGTGCGTGGCTACGTGTTCGCCAGCTACCTGATCAGCGGTCTGTGCGGCAGCCTGGCCGCGTTTTGTTCGGCGGCCTGGTTCAGCGGCAGCACCACCGGTTCCGGCGAGATGCTGCTGTCCGTGGTGGCCATCGCTTTTCTTGGGGTGATCTTCTCCCAGCGTCTGCAGCCGAGCATCGGCGGCACGCTGCTGG  >  KT2440/3138237‑3138499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑