| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,805,741 | A→T | 52.2% | intergenic (+161/‑588) | dsbG → / → CJ019_04513 | Thiol:disulfide interchange protein DsbG/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,805,741 | 0 | A | T | 52.2% | ‑2.2 / 3.3 | 15 | intergenic (+161/‑588) | dsbG/CJ019_04513 | Thiol:disulfide interchange protein DsbG/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base T (3/3); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAG‑C‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGGAGACACATTGGT > KT2440/4805607‑4805869 | gcCGTGAAGCAGGCGACGTGGGGCAGGGCACCGGCTACGCCGGTGTTCGCGGGCGAACCCGCTCCTACGGGGCGCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTa < 1:127697/135‑1 (MQ=255) gAAGCAGGCGACGGGGGGCATGCCACCGGCACCGCCGGTGTGCGCGGGTCAACCCGCTCCTACGGGGCGCGCGCTAACGGCGGGGTTTTCCGCCAGGCCCGTGAGCGCAAGGCCTGCGCGGTCGGCCGTAGGAG‑C‑g < 8:137536/136‑1 (MQ=255) aaGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGcg‑c‑gc > 8:68025/1‑135 (MQ=255) aaGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGGG‑C‑gg > 8:423978/1‑136 (MQ=255) aaGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCCTacggcgc > 2:16490/1‑129 (MQ=255) aCGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTGGGGG‑CGGGGTTTAcc > 3:113745/1‑135 (MQ=255) cACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGCGCC‑GGGTTTACCCCGCAAAGGCCCg > 4:371430/1‑135 (MQ=38) ggTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTTGGCGCC‑GGGTTTTCCCCGGAAAGGGCCGGCCCGGGGGGCCa > 4:68438/1‑135 (MQ=255) ggTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTGGGGG‑C‑GGGGTTTCCCCCGAAACGGCCGGGCCGGCggggcca > 4:348301/1‑131 (MQ=255) ggttggcgcCCGGCCGGTGAGCGCACGCCCGGCGCGGGCGGCCGTAGGCG‑G‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa < 1:152053/130‑1 (MQ=17) gTTTTGCGCCAGGCCGGTGATCGAACGGCCTGGGCGGGCGGCCGTAGGCG‑C‑GGGTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTaa < 2:267829/135‑1 (MQ=17) cAGCCCGGTGAGCGAACGCCCGGCGGGGGCGGCGGAAGCGG‑C‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCt < 8:364365/136‑1 (MQ=14) atggCCTGCGGGGGCGGCGGTTGTCG‑C‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCggggg < 6:184971/133‑1 (MQ=17) gcggGGGCGGCCGTTGGTG‑G‑GGTTTAACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGGAGac < 3:68438/135‑1 (MQ=18) ggcccGTTGTAG‑G‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGGAGACACATTGGt < 7:72786/133‑1 (MQ=255) gCCGTAGGAG‑C‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTa > 7:110376/1‑84 (MQ=255) gCCGTAGGAG‑C‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTa < 8:110376/84‑1 (MQ=255) | GCCGTGAAGCAGGCGACGTGGGGCATGGCACCGGCTACGCCGGTGTTCGCGGGTGAACCCGCTCCTACGGGGCTCGCGCTAACGGCTGGGTTTTGCGCCAGGCCCGTGATCGCAAGGCCTGCGCGGTCGGCCGTAGGAG‑C‑GGGTTTACCCGCGAAAGGGCCGGACCTGCTGGCCAATTTCCACCAGCCGTTCACCGTTGCCGCCGCCACTGTATTCGCGGGTAAACCCGCTCCTACGCTGATCGGGGGTGGAGACACATTGGT > KT2440/4805607‑4805869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |