| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,942,999 | A→C | 39.6% | L51R (CTG→CGG) | fliN ← | Flagellar motor switch protein FliN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,942,999 | 0 | A | C | 39.6% | 21.8 / 2.6 | 31 | L51R (CTG→CGG) | fliN | Flagellar motor switch protein FliN |
| Reads supporting (aligned to +/- strand): ref base A (9/8); new base C (2/11); total (11/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.75e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATCGGCCAGTGCCTG > KT2440/4942869‑4943114 | gATGTTGATCTCGGTGCTGCCCACTTCCTTGGAATTGTGCACCGGGATGTCCAGGATCAGGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑ggcg < 6:102712/135‑1 (MQ=255) aTGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑ggcgg > 5:31791/1‑135 (MQ=255) aCTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCGGGCCGcggctgctggcc > 5:73970/1‑132 (MQ=255) cATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTcc > 8:448393/1‑136 (MQ=255) gcaaaTGTTCACCGGGATGGCCTGGACCACGTCCAGGTGCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCTAATTCTTCCATCGGCC‑GGCGGCCGGGGCCGCTGCTGCTGCCGGTGTcccc < 4:81417/133‑1 (MQ=255) aaaTGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCGGGCCGCGGCGGCGGCCGGTGTcccccc > 5:201158/1‑133 (MQ=255) attGTTCACCGGGATGGCCAGGATCCCGTCCAGGTGCGGGCCTTCGCGGCTGACATTTTCATTCGGCTGCGGGGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGcc < 8:124740/133‑1 (MQ=255) tgtcccccGGGATGCCCCGGAGCCCGTCCCGGTGCGGGCCTGCGAGGCTGACATTTTCATTCGGCTGCGGCGCGCTGGCTAATTCTCCCATCGGCC‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCca < 6:73970/129‑1 (MQ=255) aCCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTg > 8:323601/1‑136 (MQ=255) gggATGTCCAGGAACACGTCCAGCTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCC‑GGCGGCCCGGCCCGCTGCAGCTACCGGCGGCCCCGCCcaccagagta > 3:215722/1‑131 (MQ=255) gATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCGGGCCCCAGCTGGGGGCGGTGGCCCCCCCCACCCGTGCagc > 3:356483/1‑133 (MQ=255) tCCAGGTTCGGGCCTTCGTTGCTTACATTTTAATTCGGCAGCGGCGTGCTGGCGAATTCTTCCCTCGGCAGGGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGcc < 2:185908/135‑1 (MQ=255) ccAGGTGCGGGCCTTCGAGGCTGACAGTTTCATTCGGCTGCGGCGAGCTGGCGCAGTCTTCCCTCGGCC‑GGCGGCCCCGTCCCCAGCGGCCGCCCTGGTCCCCCCCCACCCGGGCCCCGCCGTCGgccgggccgg > 6:29370/1‑135 (MQ=255) gggCCTGCGAGGCTGACATTTTCATGCGGCGGCGGCGCGGGGGCGATTTCTTCCATCGGCC‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCg < 3:109319/135‑1 (MQ=255) ccTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCCGGCCCCGGCGGCTGCCGGTGTCCCCCCCCCCCCGGGGCTCGCTGTCGGCCGGGCCGGCAGAccccggtt > 7:206437/1‑131 (MQ=255) gttaCATTTTCATTCGGGTTCGGGGTGCTGGCGAATTCTTCCTTCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACg < 8:321747/133‑1 (MQ=255) aTTTCCATGGGGCTTCGGCGCGGTGGCGAATTGGTCCCCCCGCC‑CGGGGGCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCg < 7:16160/136‑1 (MQ=255) aTTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCGCGCCCCTGCGGCGGCCGGGGTGCCCCCCCCGCCGAGGCTAGCAGTAGGCCTGGGCGGCAGAACCGGTGGGTTGCAACGCCGCGGccccc > 8:16160/1‑134 (MQ=255) ttcggctgcggcGAGCGGGCTAATTCTTCCATCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCAc < 6:138625/135‑1 (MQ=255) cggctgcgggGTGCGGGCTATTTCTCCCAGGGGGC‑GGGGGCCGGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCAc < 1:256174/133‑1 (MQ=255) gcttcggcGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCCGGCCGCTTCTGCTGCCGGGGTCCCCGCCCAGCACGGCATCGAAGTCGGCCTGGCCCGCACCACCACTTTCTTGCAACGCTGCGGCCcacacaa > 4:54054/1‑134 (MQ=255) gctgcggcTCGCTGGCTATTTCAGCAAGCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCTGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGTCCCACTCATc < 7:134673/136‑1 (MQ=255) gtggtgcGAGCGGGCTATTTCTCACACGGGCT‑GGGGGCCGGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATc < 6:424871/130‑1 (MQ=255) ctgcggcGAGCTGGCGAATGCTGCCAGCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATc < 4:32749/132‑1 (MQ=255) atgcggcGAGCTGTCGAATGCTTCCATCTGCC‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATc < 2:117405/132‑1 (MQ=255) ttgggcGCGCGGGCTATTTCTTCCAGCGGCC‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATCg < 3:261325/132‑1 (MQ=255) gggggcGCGCGGGCTAATTCTCCCCGCGGCC‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATCgg < 7:448393/133‑1 (MQ=255) gcgCTGGCTAATTCTCCCCGCGGCC‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATCGGCCAGt < 3:260086/133‑1 (MQ=255) gcgggCGAATTCTTCCCGCGGCC‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATCGGCCAGTGc < 3:253145/132‑1 (MQ=255) gCTGGCGAATGCTTCCATCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATCGGCCAGTGc < 4:140724/135‑1 (MQ=255) tGGCGAATTCTTCCATCGGCC‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATCGGCCAGTGCCTg < 8:206437/136‑1 (MQ=255) | GATGTTGATCTCGGTGCTGCCCACTTCCATGGAAATGTTCACCGGGATGTCCAGGATCACGTCCAGGTTCGGGCCTTCGAGGCTGACATTTTCATTCGGCTTCGGCGAGCTGGCGAATTCTTCCATCGGCA‑GGCGGCCCGGGCCGCTGCTGCTGCCGGTGTCCCCGCCCAGCAGTGCATCGATGTCGGCCTGGCCGGCAGAACCGGTTTCTTCCAACGCTGCGGCCCACTCATCGGCCAGTGCCTG > KT2440/4942869‑4943114 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |