| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,975,253 | A→C | 32.7% | Q688P (CAG→CCG) | sasA_19 → | Adaptive‑response sensory‑kinase SasA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,975,253 | 0 | A | C | 32.7% | 18.5 / 2.5 | 34 | Q688P (CAG→CCG) | sasA_19 | Adaptive‑response sensory‑kinase SasA |
| Reads supporting (aligned to +/- strand): ref base A (6/13); new base C (10/2); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.15e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCA > KT2440/5975121‑5975386 | gcgcTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCa < 3:340945/133‑1 (MQ=255) gcTGCCTCCCCGCAACGGCGCCCGACCGGCGTGGGCGTCCCTCATGCCCGGGGCATGGCCGCCGCTTAGGCACACACGCCCGTGCACCAGCGCGCCTCGCGGCATGCACGTAGCGCCCTGGCCATGCGCCAGGCt < 2:292883/135‑1 (MQ=255) gggCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGGCCGGCGCCCGGCGTGG‑CCAGGGCGCACGcc > 1:16143/1‑134 (MQ=255) cTTGGGATTCACCCATGACCGGGTGATGGCGGCCGCTTATCCAGTCCCGCCCGGGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCACTGCGCCTGGCTTGG‑CCAGGGCGAACGCACGAGGTGTTGTTc < 8:255755/136‑1 (MQ=255) gggCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCCTGCGCCAGGCATGG‑GCAGGGCGCACGCCCGCGGTGTTGTTagag > 8:229917/1‑136 (MQ=255) ggCTTCACTCATGCCCGGGTGATGGAGGCCGCTTATCCACTCACGCCCGGGCACCAGCGCGCCGCGCTGCATCCACGTAGCGCCCTGGCCAGGCGCCAGGCTGGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGa < 4:131160/134‑1 (MQ=255) cTTCCCTCATGCCGGGGGGTGGGCGGCCGCTTAGCCACCCCCGCCCGTCCACCAGCGCGCCTCGGTGCAGGCCCGAAGCGCCCTGCCCATGCGCCAGGCTGGG‑CCCGGGCGAACGCACGAGGTGTTGTTCGAGCt < 6:322490/135‑1 (MQ=255) tCACTCATGAGCGGGGGATGGAGCCCGATTAAGAACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCATGCGCCAGGCTTGG‑CCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCgc < 8:88961/136‑1 (MQ=255) tCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCGGGCCCTGCGCCAGGCTTGG‑GCAGGGCGCACCCACCCGGTGTTGGTGGAGCTCg > 6:308734/1‑135 (MQ=255) ccggggTTAGGGCGGCCGCTTATCCACTCACGCCCGTGCACCCGCGCGCCGCGCTGCAGGCACGTATCGCCCTGCCCATGCGCCAGGCTTGG‑CCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCcagccagc < 1:43962/132‑1 (MQ=255) cccGGGTGATGGAGGCCGCTTATGCAGTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCTCGTATCGCCCTGGCCAGGCGCCAGGCTTGG‑CCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCt < 7:229917/135‑1 (MQ=255) aCCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGGCCTGCGCCCGGCTTGG‑GCCCGGCGCACGCCCGAGGGGTTGTTGGAGCTCgccagcccg > 5:231751/1‑132 (MQ=255) aCCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCCTGCGCCCGGCGTGG‑CCAGGGCGAACGCCCGAGGTGTTGTTCGAGCTGGCcagcccgcg > 7:328778/1‑135 (MQ=255) aCCGGGGGATGGCGGCCGCTTCCGCCCGCACGCCCGTCCACCAGCGCGCCTCGCTGCATGCCCGTATCGCCCGGGCCTTGCGCCAGGCGTGC‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCt < 8:230936/136‑1 (MQ=255) aCCGGGGGAGGGAGGCCGCTTATCCACTCACCCCCGTGCACCAGCGCGCCTCGCTGCAGCCCCGTAGCGCCCGGCCAATGCGCCAGGCTTGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCt < 8:275698/136‑1 (MQ=255) ccGGGGGAGGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTAGCGCCCTGGCCATGCGCCAGGCTTGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCt < 4:134288/135‑1 (MQ=255) gggTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCATTCGCCCCGCTTTG‑GCCGGGCGAACGCACGAGGTGTTGTTCGAGCGCGCcagccagcga > 2:322319/1‑132 (MQ=255) gTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCATGCGCCCGGCGTGG‑CCCGGGCGGACGCCCCAGGTGTTGTTAGGGCTCGCCCGCCAGCTACCAc > 7:275698/1‑136 (MQ=255) gATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCAGCGCCCGGGGTGG‑CCCGGGCGGACGCACCCGGGGTTGTTGGCGCACGCCCCCCCGCCACAAcc > 4:323831/1‑134 (MQ=255) aTGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCAGGGCCATGCGCCCCGCTTTT‑CCACGGGGCAAGCCCGACGTGTGTTTAGCGCGCGCCAGCCAGCGACacaac > 2:111428/1‑131 (MQ=255) tGGCGGCCGATTATGCACACCCCCCCGGGCACCCCCGCGCCGCGCGGCAGGCACGAACCGCCCTGCCCATGCGCCAGGCTGGG‑CCTGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAAcgcg < 3:187903/135‑1 (MQ=255) gggttATGCACTCACCCCCGGCCACCCGCGCGCCTCGCGGCAGCCACTTAGCGCCGGGCCAAGGCGCCTGGATTGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGccc < 4:442660/132‑1 (MQ=255) ggCTTATGCACTCACCCCCGGGCACCCGCGCGCCGCGCGGCAGGCACGTATCGCCCTGGCCAGGCGCCTGGCTGGG‑CCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGccc < 5:228021/134‑1 (MQ=255) cTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCAGGCCCTGCGCCCGGCTTGG‑CCCGGGCGCACGCCCGCGGGGTTGTTGGGGCTCGCCAGCCCGCGACCACGCGCCCGccccg > 6:394476/1‑134 (MQ=255) gccccccACGCCCGGGCACCAGCGCGCCGCGCTGCAGGCACGTAGCGCCCTGCCCAGGCGCCCGGCTTGG‑CCCGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGc < 3:323831/127‑1 (MQ=255) aCGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCa < 3:58369/56‑1 (MQ=255) cccGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTT‑G‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCgg > 4:31324/1‑135 (MQ=255) ccAGCGCGCCGCGCTGCTTGCACGTAGCGCCCTGGCAATGCCCCAGGCTTGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTg < 3:207749/135‑1 (MQ=255) cgcgcCTCGCTGCATGCACGTAGCGCCGTGGACAGGCGCCAGGCTTGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGaa < 6:30347/135‑1 (MQ=255) cgcCTCGCTGCATGCACGTATCGCCCAGGGCCAGCGCCCGGCGTGG‑GCAGGGCGCCCGCCCGCGGTGGTGTTCGCGGTCGCCCGCCCGCATCACCCCGGCCGCCCCGGCGGGGTTTGAGCGGGTGTCTGGGGAGCg > 8:210590/1‑136 (MQ=255) tCGCTGCATCCACGTATCGCCCTGGCCATGCGCCAGGCTGGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTc < 2:308375/135‑1 (MQ=255) cGCCCTGGCCATGCGCCCGGCGTGG‑CCCGGGCGCACGCCCGAGGTGTTGTTCGAGCTCGCCCGCCAGCTACAACGCGCCAGCCCCGGCGGGGTTGGAGCGGTTGACTGCGAAGCGTTCCAGCAGCGTTTACGCGa > 4:354665/1‑135 (MQ=255) gccccggccccgCGCCCGGGTGGGCCCCGGGCCAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTCCGCGa < 1:198210/135‑1 (MQ=255) ccAGGCTTGG‑CCAGGGCGCACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGCCTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCa > 8:353491/1‑136 (MQ=255) ccAGGCTTGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATgcgc > 1:345867/1‑135 (MQ=255) ccAGGCTTGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCa > 7:125372/1‑136 (MQ=255) | GCGCTGGCTTCCTGCAACCGGGCCAGAACGGCTTGGGCTTCACTCATGACCGGGTGATGGAGGCCGCTTATGCACTCACGCCCGTGCACCAGCGCGCCTCGCTGCATGCACGTATCGCCCTGGCCATGCGCCAGGCTTGG‑CCAGGGCGAACGCACGAGGTGTTGTTCGAGCTCGCCAGCCAGCTACAACGCGCCAGCCCCTGCGGGTTTGCAGCGGATGACTGCGAAGCGTTCCTGCAGCTTTTACGCGAAGCCGCATCATGCGCA > KT2440/5975121‑5975386 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |