Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,718,463	T→G	32.8%	Q65P (CAG→CCG)	CJ019_01614 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,718,463	0	T	G	32.8%	29.8 / 3.2	45	Q65P (CAG→CCG)	CJ019_01614	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (11/16);  new base G (6/10);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGG  >  KT2440/1718337‑1718596                                                                                                                               |                                                                                                                                       atgaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGgcgcg                                                                                                                                  >  4:291911/1‑133 (MQ=255) atgaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGAGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTCgagcgc                                                                                                                                 >  8:259147/1‑134 (MQ=255)  tgaACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGGGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAg                                                                                                                              <  8:116376/136‑1 (MQ=255)   caaCAGCAACTTGCCATGCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCGGCGCGGGGTCATCGGTGAGAGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAg                                                                                                                              <  3:12085/134‑1 (MQ=255)         cAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTcca                                                                                                                        >  6:109473/1‑133 (MQ=255)                      aGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCGGGCGCGCCAGGGTCACGGCC‑GGGGCggg                                                                                                           >  5:214023/1‑132 (MQ=255)                            aTGCGCGCACTTCCCGCTTGGGGGGGGCCCGGCTTCACCTGCGGGGGGTCATAGGGGAGAGGCACCGGCCGCGGCAGGTGGCGTTTGACCGGGCCGGCGGGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCa                                                                                                      <  1:131433/133‑1 (MQ=255)                               cgcgcACTACCCGCATAGCGGGGGCCAGGCGGGACCTGCGCGGGGGCATCGGTGAGATGGACCGGCAGCGGCACGTTGCCTTGGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGac                                                                                                 <  1:419196/135‑1 (MQ=255)                                gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCACGGTCCCGGCC‑GGGGCGTCCATCAGAGccac                                                                                               >  7:88407/1‑136 (MQ=255)                                gcgcACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCA‑GGGGCGGCCCTCAGCGaca                                                                                                >  6:94641/1‑135 (MQ=255)                                 cgcACTTCCGGCATAGGGGTGGCCCGGCTTGCCCTGCGGGGGGTCATCGGGGAGCGGGAACGGCAGCGGCACGGGGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGacac                                                                                               <  4:1183/135‑1 (MQ=255)                                    aCTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATc                                                                                            <  2:15981/135‑1 (MQ=255)                                     cTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCt                                                                                          <  7:202779/136‑1 (MQ=255)                                      ttACGGCATTGCGGTGGCGAGGCTTGCCGTGCTGGGGGTCTTTGTTGTGATGGAAGGTCAGCGGCACGTTGCCTTTTACCGGGCCTGCCTGGCGGGCCAGGGTCACGTCA‑GGTGCGTCCATCAGCGACAGATCCt                                                                                          <  4:97286/135‑1 (MQ=255)                                          cGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGGGCGCCAGGGGCACGGCC‑GGGGCGGCCCTCAGCGCCACCTCCTGCAg                                                                                      >  3:107544/1‑135 (MQ=255)                                                 ggggggCCCGGCTGGCCGTGCGGGGGGGCATAGGGGAGATGGAACGGCAGCGGCACGTTGCCTTTGACGGGGCCGGCCGGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCAc                                                                                <  5:48714/130‑1 (MQ=255)                                                 cGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCCCg                                                                               >  4:135212/1‑135 (MQ=255)                                                         aGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCGGGTCCCGGCC‑GGGGCGGCCCGCCGCGCCCCAGCCGGCCGTTCCACGCTCAGGGt                                                                       >  5:187704/1‑135 (MQ=255)                                                         aGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACCCATCCAGCAGTTCCCCGCTCAGGGt                                                                       >  1:271209/1‑135 (MQ=255)                                                         aGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGGGACCGCA‑TGGGCGTCCAAAAGCCGCCCATCCTGCCGTTGCACGCGGAgggg                                                                       >  2:409253/1‑134 (MQ=255)                                                         aGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGGGCCGGCC‑GGGGCGGCCCTGAGCGCCACCACCAGCCGGTACACGCGCAGGGt                                                                       >  6:288388/1‑135 (MQ=255)                                                            cTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcg                                                                   <  8:104679/136‑1 (MQ=255)                                                              tGACCTGCGCGGGGTCATAGGTGAGATGGAACTGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCCCGGCC‑GGGGGGTCCACGAGCGCCCCCTCCTGCAGTTAAACGCCCAGGGTggcggc                                                                 >  8:1674/1‑136 (MQ=255)                                                              tGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGGCAGGAGCGCCCCGGGCACCGGA‑GGGGCGGGCATCCGGGCCACATACTGCCGTGCCCAGCAGAGGGTgggcg                                                                  >  6:7634/1‑132 (MQ=255)                                                                 ccggcgcGGGGTCATCGGTGAGCGGCCACGCCAGCGGCACGTTGCCTTGGCCCGGGCCGGCGGGGCGCGCCAGGGGCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                              <  7:169416/133‑1 (MQ=255)                                                                 ccTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                              <  7:21114/136‑1 (MQ=255)                                                                  cggcggGGGGGCATCGGGGCGCTGGCCCGGCCGCGGCACGTGGCCTTGGCCCGGGCCGGCCGGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTggcggcagg                                                              <  5:322613/133‑1 (MQ=255)                                                                        ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCCCGGGCACGGCA‑GGGGCGGCCCTCAGCGCCACATCCTGCAGTTCCACGCGCCGGGGGGcggccggcggcagg                                                       >  8:6883/1‑136 (MQ=255)                                                                        ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCACGGTCACCGCC‑GGGGCGGGCCTCACCGCCCCCACCGGCACTTCCACGCGCAGGGGGgcggccggcggcag                                                        >  6:322613/1‑135 (MQ=255)                                                                        ggggTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCCGGGTCCCGGCC‑GGGGCGGCCCTCAGCGCCCCAACCAGCACTTCCACGCGCAGGGGggcggcaggcggccg                                                        >  5:234030/1‑133 (MQ=255)                                                                                aGGTGAGATGGAACGGCAGCGGCACGTTGCCGTTGACCGGGCCGGCCGGGCGCGCCAGGGGCACGGCA‑GGTGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgc                                                <  6:202969/135‑1 (MQ=255)                                                                                  gggagaGGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                              <  1:12319/133‑1 (MQ=255)                                                                                  gTGAGATGGAACGGCCGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATgcgc                                              <  7:6883/135‑1 (MQ=255)                                                                                       aTGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCAGGCGCCGCAGGCTGACGGCACCGGGCG‑CGAAAACCGACACATACTGCAAGTCCACCCCCACGCTGGCGGCAGGCGGCAGGGCCATacgcgtcag                                         >  4:67777/1‑130 (MQ=255)                                                                                        tGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑TGGGCGGGCATTAGCGCCACCTCCTGGAGTTCCACGGACAGGGGGGCGGCAGGCGGCAGGGCAATTCGCTGCAgg                                        >  6:334283/1‑135 (MQ=255)                                                                                            aCGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTaga                                    <  6:145472/135‑1 (MQ=255)                                                                                                 cgCGGCACGTGGCCTTGGCCCGGGCCGGCCGGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                               <  5:288388/134‑1 (MQ=255)                                                                                                 aGCGGCTCGTTGCCTTTGACCGGGCCGGCCGGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                               <  3:304683/135‑1 (MQ=255)                                                                                                 aGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACt                               <  6:234030/135‑1 (MQ=255)                                                                                                               tttGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCt                 <  4:87104/135‑1 (MQ=255)                                                                                                                  gcccGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGCGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTg                <  6:345872/131‑1 (MQ=255)                                                                                                                    gcgggcgggGCTGGGGCGCGAGGGTCACGGCA‑GGGGGGTCCATCAGCGAGAGATCGTGCAGTTCCACGCTGAGGGTGGCGGCAGGCGGCAGGGCTATGGGCTGGAGGTAGAAGACTTCACCGTCCAGGCTGGcgt            <  3:67777/134‑3 (MQ=255)                                                                                                                       ggccggccggccGCGCCAGGGGCACGGCA‑GGGGCGGCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGt         <  2:197957/135‑1 (MQ=255)                                                                                                                       ggccggccGGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCTGGGTGGCGGCAGGCGGCAGGGCTATGCGCTGCAGGTAGAATACTTCACCGTCCAGGCTGGCCTGGtt        <  7:259147/136‑1 (MQ=255)                                                                                                                        gccggccTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGtt        <  3:291911/135‑1 (MQ=255)                                                                                                                              cTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTtgggtgg  <  5:194751/135‑1 (MQ=255)                                                                                                                               |                                                                                                                                       ATGAACAGCAACTTGCCATTCAGCTCAATGCGCGCACTTACCGCATAGCGGTGGCCAGGCTTGACCTGCGCGGGGTCATAGGTGAGATGGAACGGCAGCGGCACGTTGCCTTTGACCGGGCCGGCCTGGCGCGCCAGGGTCACGGCA‑GGGGCGTCCATCAGCGACACATCCTGCAGTTCCACGCTCAGGGTGGCGGCAGGCGGCAGGGCAATGCGCTGCAGGTAGAAGACTTCACCGTCCAGGCTGGCCTGGTTGGGTGG  >  KT2440/1718337‑1718596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑