| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 223,541 | T→G | 18.9% | V52G (GTC→GGC) | oprM → | Outer membrane protein OprM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 223,541 | 0 | T | G | 18.9% | 97.7 / 2.6 | 63 | V52G (GTC→GGC) | oprM | Outer membrane protein OprM |
| Reads supporting (aligned to +/- strand): ref base T (31/18); new base G (11/3); total (42/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCCTGTGCCTGTCGCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCAACATTGCCAAAGCTCGCGCTCAGG > KT2440/223408‑223663 | ggcCTGTGCCTGTCGCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTc > 4:46712/1‑135 (MQ=255) ctgtCGCTGGGGGCCTGCATGATGGTGGGGCCCGAGTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTACCCCAGGAcg > 8:318210/1‑135 (MQ=255) cTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGTCCTGAACGGCCCGCTGCGCCCGGCTGCCGCCAGCGTCGTGGCACCGCCGGGGCCCGGGGCCaggtgg > 5:121531/1‑135 (MQ=255) gggCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCa < 4:95205/133‑1 (MQ=255) gCCTGCATGAGGGGGGGGCCCGACTATGAGGTGCCTGGGGAGGCGGCCGTGCAACGCAAGGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGGCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑Tg < 6:288044/135‑1 (MQ=255) gatgGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTGAGCGCCGGGCCCCGCGGGCTGGGGGCCGC‑TGTATCAggg > 3:249620/1‑134 (MQ=255) atgGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGAt < 8:198261/135‑1 (MQ=255) ggCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGc > 3:218204/1‑135 (MQ=255) cccGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGCTGCCTGGTGGCCGC‑AGGATCCCGATCACCGGcggc > 7:333781/1‑133 (MQ=255) cccGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACtggt < 8:232404/109‑1 (MQ=255) cccGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACtggt > 7:232404/1‑109 (MQ=255) cccGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTg > 7:67680/1‑135 (MQ=255) cccGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGGGGCAGC‑TGTATCAGGATCAGCGGCt > 6:196893/1‑134 (MQ=255) cccGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGGCCCCGAGGACTGGGGGCAGC‑GGTATCAGGATCAGCGGCTg > 3:427559/1‑135 (MQ=255) cGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCCAGGACTGGTGGCAGC‑TGTATCAGGATCAGCCGCTGaa > 1:27507/1‑135 (MQ=255) gACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGGCCCCGAGGCCGGGTGGCAGG‑AGTATGAAGATCAGCGGGGGAAc > 4:115235/1‑135 (MQ=255) cTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGCGGACTGGTGGCAGCGTGT‑TCAGGGTCAGCGGCGGCACgc > 6:412201/1‑134 (MQ=255) tGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGc < 3:46712/135‑1 (MQ=255) cgggggAGGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCATC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTc < 2:173567/133‑1 (MQ=255) tGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGGCCCCGCGGCCGGGTGGCCGG‑GGTATCAGGATACGCGGCTGCACGCGCTGGTGCGTccg > 8:398257/1‑134 (MQ=255) ggggATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGCCAGCGTCGTGTCAGCGCCCGTCCCCGCGGCCTGGTGGCCGC‑TGTTTGAGGATAAGCGGCTGGACGCGCGGGTGCGTccg > 6:56947/1‑133 (MQ=255) ggggATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGCCTGGTGGCAGC‑TGTTTCAGGATCAGCGGCTGCACGCGCTGGTGGGTCAg > 1:300798/1‑135 (MQ=255) ggggATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGGCCCCGAGGGCTGGTGGGAGC‑GGTTTGAGGGTAAGCCGCAGAACGAGCGGGTGCGTCCgg > 8:259826/1‑136 (MQ=255) ggggATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCCGTACCCGAGGACTGGTGGGCGC‑AGTATCAGGCACCGCGGGGGACCGCGCTGGGGCCTCAgg > 8:421796/1‑136 (MQ=255) ggggATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCCGGCCCCGCGGCCTGGTGGCAGC‑GGTTTCAGGATCAGCGGCGGGACGAGCTGGTGCGTCAg > 1:285368/1‑135 (MQ=255) ggggATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCCCCGGTGCCCGCGGACGTGTGGCAGC‑AGTATCAGGATCAGCGGCTGAACGAGCGGGGGCGTCAg > 1:316592/1‑135 (MQ=255) gATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCCGGGCCCGAGGACTGGTGGCCGC‑GGGATCAGGATCAGCGGGGGCACGCGCTGGTGCGTCAGgcg > 2:43607/1‑135 (MQ=255) ggCCGTGCAACGCATTGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGGCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTAGCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAg < 3:104993/135‑1 (MQ=255) ggCCGTGCAACGCAATGCCCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGGTAGCGCCGCTCCCCCAGGACCGGTGGGAGC‑TGTTTAAGGATCACCCGCAGCACGACCAGGCGCGTCCCGCGCTgcg > 6:399866/1‑133 (MQ=255) ggCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAg < 6:244168/135‑1 (MQ=255) tGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCa < 4:49592/135‑1 (MQ=255) aaCGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGCGGTCTGGTGGCAGC‑TGTATCATGATCAGCGGCTGAACGCCCGGGTGCGTAAGGCGCTGAGTGCCAaca > 4:172944/1‑135 (MQ=255) aaCGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACtggtgg < 4:392621/77‑1 (MQ=255) aaCGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACtggtgg > 3:392621/1‑77 (MQ=255) aaCGCAATGACCTGAACGGCCCGCTGCGCCACGATGCCGACACCGTCGTGTACGCCCCGGTCCCCCACGCCGGGTGGGAGC‑GGGATAATGATCAGCGGCTTCACGAGCTGGGTCGTCACGCGCAGAGTGCCcaca > 4:328071/1‑135 (MQ=255) ccTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGCGCGGGTGCGTCAGGCGCGGAGTGCCAACACCCAACTgcg > 4:339060/1‑135 (MQ=255) ccTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTgcg > 6:92349/1‑135 (MQ=255) ccTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCCCCGGTCCCCGAGGACTGGTGGCCGG‑TGGATCAGGATCAGCGGCGGCACGCGCGGGTGCGGCAGGCGCAGAGTGCCCACCCCGAACTgcg > 1:437069/1‑135 (MQ=255) aCGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGc < 1:299694/84‑1 (MQ=255) aCGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGc > 2:299694/1‑84 (MQ=255) cGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTggcg < 7:318210/136‑1 (MQ=255) ggCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCCGCGCCGGGCCCCGCGGCCTGGGGGCCGC‑GGTATCAGGCTCAGCGGGGGCACGTGCTGGTGCGGCAGGCGCGGAGTGGCACCACCGCACAGCGCGTggcg > 5:68483/1‑135 (MQ=255) ggCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTggcg > 3:222364/1‑135 (MQ=255) gCTGCGCCAGGATGCCGACAGCGTCGTGTCCGCGCCGGTCCCCGAGGACTGGTGGCAGC‑GGTTTCAGGATCAGCGGCGGCACGGGCTGGTGGGTCAGGCGCTGAGTGCCCACCCCGACCTGCGCGTGGCGGCAGc > 4:184050/1‑135 (MQ=255) gCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGCAGACTGGTGGCAGA‑TGTATCAGGATAAGCGGCGGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAg > 2:278165/1‑134 (MQ=255) gCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGCTCAGCGGCTGAACGCGCGGGTGCGTCAGGCGCTGAGTGGCCCCCCCGCACAGCGCGTGGCGGCAGc > 4:35971/1‑135 (MQ=255) gCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATGAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGGCACCACCGAACTGCGCGTGGCGGCAg > 2:334227/1‑134 (MQ=255) gCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATAAGCGGCTGCACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCCGc > 1:80525/1‑135 (MQ=255) gCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGGTTCAGGATCAGCGGCGGAACGAGCTGGTGGGTCAGGCGCTGAGTGGCCACACCGCACTGCGCGTGGCGGCAGc > 1:73445/1‑135 (MQ=255) gCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGGCCCCGCGGGCGGGTGGGCGC‑GGTATGAGGATCAGCGGCGGAACGCGCGGGTGCGGCAGGCGCTGCGGGGCCACACCGCACTGCGCGTGGCGGCAGc > 3:10155/1‑135 (MQ=255) tGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCa < 4:148537/135‑1 (MQ=255) ggcgcCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGAGCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCa < 6:69893/134‑1 (MQ=255) gcCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCCGC‑TGTATAAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCCACACCGAACTGCGCGTGGCGGCAGCCAACa > 4:156916/1‑135 (MQ=255) gcCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑Tg > 8:284079/1‑56 (MQ=255) gcCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑Tg < 7:284079/56‑1 (MQ=255) cGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCAACATTGCCAAAGc < 2:285368/135‑1 (MQ=255) aCAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCAACATTGCCAAAGCTc < 4:10155/135‑1 (MQ=255) aCAGCGTCGGGGCTGCGCCGGGCCCCGAGGAGTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAAGGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCAACATTGCCAAAGCTc < 4:294706/135‑1 (MQ=255) cAGCGTCGTGTCAGCGCCCGGGCCCGATGACGGGTGGCACC‑GGTATCCTGAACAGCGGCTGAACGAGCTGGTGCGGAACGAGCGGAGGGCCACCACCGCACTGCGCGTGGCCGCAGCCCACAATTCCCAAGcacc > 2:270462/1‑132 (MQ=255) cAGCGTCGTGGCAGCTCCGGGCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCAACATTGCCAAAGCTcg < 2:400270/135‑1 (MQ=255) agggtcgtGTCAGCGGCGGTCCCCGAGGACGGGTGGCAGC‑TGTATCAGTATCAGCGGCTGAACGAGATGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCAACATTGCCAAAGCTcgc < 4:408916/132‑1 (MQ=255) cgtcgtGTCAGCGCCGGGCCCCGAGGACGGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCAACATTGCCAAAGCTCGCGCt < 7:259826/136‑1 (MQ=255) tgtCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGCACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCCGCCAACATTGCCAAAGCTCGCGCTCagg > 6:431414/1‑135 (MQ=255) | GGCCTGTGCCTGTCGCTGGGGGCCTGCATGATGGTGGGGCCCGACTATGAGGTGCCTGGGGATGCGGCCGTGCAACGCAATGACCTGAACGGCCCGCTGCGCCAGGATGCCGACAGCGTCGTGTCAGCGCCGGTCCCCGAGGACTGGTGGCAGC‑TGTATCAGGATCAGCGGCTGAACGAGCTGGTGCGTCAGGCGCTGAGTGCCAACACCGAACTGCGCGTGGCGGCAGCCAACATTGCCAAAGCTCGCGCTCAGG > KT2440/223408‑223663 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |