Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	5,757,239	(A)6→5	coding (781/834 nt)	CJ019_05400 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,757,234	0	A	.	92.3%	85.5 / 11.2	30	coding (776/834 nt)	CJ019_05400	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base . (19/5);  total (19/11)
Fisher's exact test for biased strand distribution p-value = 6.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATG  >  KT2440/5757111‑5757360                                                                                                                             |                                                                                                                                cgcgcgAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAAcg‑ccgc                                                                                                                     >  4:219867/1‑134 (MQ=255)                     gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGgccgccg                                                                                               >  3:233603/1‑136 (MQ=255)                         tGGCCAAGGTCTTGAAGACCATCGAGGCAACCCTCGCCCGCCAGGCCTGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                           >  7:188390/1‑136 (MQ=255)                         tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                           >  8:1181/1‑136 (MQ=255)                         tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCcgccgccgc                                                                                           >  3:240123/1‑136 (MQ=255)                         tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGCCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑‑AAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCt                                                                                          >  3:239988/1‑136 (MQ=255)                          ggCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCt                                                                                          >  2:493/1‑136 (MQ=255)                                       aaGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCggg                                                                             >  1:231777/1‑136 (MQ=255)                                        aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                            >  2:180349/1‑136 (MQ=255)                                               cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAg                                                                     >  8:237066/1‑136 (MQ=255)                                               cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAg                                                                     >  3:89020/1‑136 (MQ=255)                                               cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAg                                                                     >  4:180854/1‑136 (MQ=255)                                               cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAg                                                                     >  6:191640/1‑136 (MQ=255)                                               cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGAGACCGGGCCCgggcg                                                                     >  8:136729/1‑132 (MQ=255)                                                    acaCCCTGGCCCCCCGGCCCCGTCAAGCT‑CAGCGGCACGCCAATAAGGCCCTCCGGCCGCAGCTGTACGCGG‑AAAAACG‑CCGCCAGAAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGc                                                                <  8:102498/134‑1 (MQ=255)                                                               cgccAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTgg                                                     >  8:38734/1‑136 (MQ=255)                                                                  ccggCCCGTGAAGCTGACGCGGCACG‑CAAGACGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAc                                                  <  2:184504/134‑1 (MQ=255)                                                                       cggttaaGCTGAAGCGCCCCG‑CAAGACGGCCCGGCTGGCGCCCCCGGAAGC‑GTAAAAACG‑CCGCCAGGAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                             <  2:181688/131‑1 (MQ=255)                                                                       cggTGACGCTGAAGCGCCACG‑AAAGAAGGCCCGGCTGGCGCCGCTGGAAGC‑GCAAAACCG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                             <  4:89020/134‑1 (MQ=255)                                                                        cggtcaGCTGAAGCGCCACG‑CACTACGGCCGTGCTGGCGAAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGa                                            <  6:205002/131‑1 (MQ=255)                                                                        cGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGa                                            <  4:57282/136‑1 (MQ=255)                                                                             aGCTGAAGAGGCACG‑CATGAACGCCATGCGGGCGCAGCAGGAAGC‑GTAAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                                        <  4:187622/135‑1 (MQ=255)                                                                                  acgcggCACG‑CACGATGGCCGTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                  <  4:240123/132‑1 (MQ=255)                                                                                             cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTc                        >  8:160476/1‑136 (MQ=255)                                                                                              aagaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCACTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCg                       >  6:142492/1‑136 (MQ=255)                                                                                                 acggCCCGGCGGGCGCAGCAGCAAGC‑CAAACACCG‑CCGCCCGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                    <  3:160983/134‑1 (MQ=255)                                                                                                 aagCCCCTGCTGCCCCCCCCGGAAGC‑GCAAAAACC‑CCGCCACCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                    <  1:164524/136‑1 (MQ=255)                                                                                                           tgGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTCCCACCCTCGGGCCGATGGTTTc          >  3:49237/1‑136 (MQ=255)                                                                                                           tgGCGCAGCAGGAAGCGG‑AAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTc          >  6:102812/1‑136 (MQ=255)                                                                                                                     ctgaacGGAAAAACCCCCCGCCTGCTGGATGCGCGGGCGGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATg  <  2:245009/131‑1 (MQ=255)                                                                                                                             |                                                                                                                                CGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACG‑CAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACG‑CCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATG  >  KT2440/5757111‑5757360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑