| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,440,367 | 0 | T | G | 57.9% | ‑0.9 / 13.5 | 20 | W26G (TGG→GGG) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (11/0); total (14/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA > KT2440/3440240‑3440497 | aCGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGC‑GGCGCCCGGGCCGCCGGGGTGTgg > 5:87680/1‑136 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGATGGC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCtat > 1:9790/1‑135 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGC‑GGCGGCCGGGCCGCCGGGGTGTGGGAGGCCACCGCCtag > 4:91709/1‑133 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCGGGC‑GGCGCCCGGGCACCCGCGGTGTGGCAGGCCAACGccgaaa > 8:17491/1‑131 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGACGCCCGCGCTGGC‑GGCGCCCGGGCGGCCTGGGTGTGGCAGGCCCACCCCgat > 5:103596/1‑134 (MQ=255) aaGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGC‑GGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCACCCCGATAAGGCGCCgcgcgc > 8:127824/1‑136 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGGGC‑GGCGCCCCGGCCGCCGGGGTGTGGGACGCCCCCCCCGATAAGGCGCAGCTCGCGGAt > 8:122369/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGGGGCCCGGGC‑GCCCGGGGGTGGCGGGCCCCCGCCGCTAATGCGCCGCGCGCGGGTCCGGcc > 6:100173/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGC‑GGCGCCCGCGCCGCCGGGGGGTGGGGGGCCCCCGCCCAAAAGGCGCCGCGCGCGGCTCAGGcc > 1:45523/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGC‑GGCGCCCCGGCCGCCGGGGGGTGGGGGGCCAACGCCGATAAGGCGCCGCGCGCTGATCCGGcc > 1:72180/1‑136 (MQ=255) gAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCAGGC‑GGCGGCCCGGCAGCCGGGGTGTGGCCGGGCCACGCCGATAAGGCGCCGCGGGCGGATAAGGcc > 8:58505/1‑134 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGC‑GGCGCCCGGGCCGCCGGGGTGTGGCCGGCCCACCCCAATAAGGCGCAGCGCGCTGCTCAGGCCGCTGGCGTCGGCCAGCAGt > 7:237705/1‑136 (MQ=255) ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGC‑GGCGGCCGGGCTGCCGGGGTGTTGCAGGCCCACCCCAATAATGCGCAGCTCCCGGATCAGGCCGCTGACGTCGGGCAGCAGTTgggg > 6:229327/1‑134 (MQ=255) cGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGC‑GGCGCCCGGGCCGCCGGGGGGTGGGGGGCCCACGCCGATAAGGGGCCGCGCGCGGAGCAGGCCGCGGAGTTCGGGCAGCAGGTGggggggcggg > 8:72598/1‑130 (MQ=255) cgcgggggCGCGGCGGCGGGCCGCCCGCGCGGGC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgcg < 1:129534/130‑1 (MQ=255) gcgggCCGCCCGCGCGGCC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg < 7:127824/133‑1 (MQ=255) gcgggc‑ggcGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcag < 6:87680/133‑1 (MQ=255) c‑ggcgCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcagca < 2:137794/136‑1 (MQ=255) cgccgcCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTgcagcagca < 1:223259/133‑1 (MQ=255) cAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCa < 5:229327/136‑1 (MQ=255) | ACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAAGGCTGCAGCAGCAGCGCTCGATCA > KT2440/3440240‑3440497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |