| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 3,440,343 | A→C | S18R (AGC→CGC) | CJ019_03266 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,440,343 | 0 | A | C | 89.5% | 17.2 / ‑3.2 | 19 | S18R (AGC→CGC) | CJ019_03266 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base C (15/2); total (17/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01 | |||||||||||
GATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGG > KT2440/3440211‑3440468 | gATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTCGccgcccgcc > 8:222442/1‑135 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGC‑GGCGCCCGGGcc > 4:112625/1‑135 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGC‑GGCGcccccgcc > 2:29019/1‑131 (MQ=255) aTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCTGGC‑GGCGCCCCGGcc > 6:196812/1‑135 (MQ=255) aCGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGC‑GGCGCCCGGGCCGCCGGGGTGTgg > 5:87680/1‑136 (MQ=255) cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGATGGC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCtat > 1:9790/1‑135 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGC‑GGCGGCCGGGCCGCCGGGGTGTGGGAGGCCACCGCCtag > 4:91709/1‑133 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCCCGGGC‑GGCGCCCGGGCACCCGCGGTGTGGCAGGCCAACGccgaaa > 8:17491/1‑131 (MQ=255) aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGACGCCCGCGCTGGC‑GGCGCCCGGGCGGCCTGGGTGTGGCAGGCCCACCCCgat > 5:103596/1‑134 (MQ=255) aaGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGC‑GGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCACCCCGATAAGGCGCCgcgcgc > 8:127824/1‑136 (MQ=255) agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGGGC‑GGCGCCCCGGCCGCCGGGGTGTGGGACGCCCCCCCCGATAAGGCGCAGCTCGCGGAt > 8:122369/1‑135 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGCGGGGGCCCGGGC‑GCCCGGGGGTGGCGGGCCCCCGCCGCTAATGCGCCGCGCGCGGGTCCGGcc > 6:100173/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGC‑GGCGCCCGCGCCGCCGGGGGGTGGGGGGCCCCCGCCCAAAAGGCGCCGCGCGCGGCTCAGGcc > 1:45523/1‑136 (MQ=255) tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGC‑GGCGCCCCGGCCGCCGGGGGGTGGGGGGCCAACGCCGATAAGGCGCCGCGCGCTGATCCGGcc > 1:72180/1‑136 (MQ=255) gAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCAGGC‑GGCGGCCCGGCAGCCGGGGTGTGGCCGGGCCACGCCGATAAGGCGCCGCGGGCGGATAAGGcc > 8:58505/1‑134 (MQ=255) gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGC‑GGCGCCCGGGCCGCCGGGGTGTGGCCGGCCCACCCCAATAAGGCGCAGCGCGCTGCTCAGGCCGCTGGCGTCGGCCAGCAGt > 7:237705/1‑136 (MQ=255) ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGC‑GGCGGCCGGGCTGCCGGGGTGTTGCAGGCCCACCCCAATAATGCGCAGCTCCCGGATCAGGCCGCTGACGTCGGGCAGCAGTTgggg > 6:229327/1‑134 (MQ=255) cGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGC‑GGCGCCCGGGCCGCCGGGGGGTGGGGGGCCCACGCCGATAAGGGGCCGCGCGCGGAGCAGGCCGCGGAGTTCGGGCAGCAGGTGggggggcggg > 8:72598/1‑130 (MQ=255) cgcgggggCGCGGCGGCGGGCCGCCCGCGCGGGC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgcg < 1:129534/130‑1 (MQ=255) gcgggCCGCCCGCGCGGCC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCgg < 7:127824/133‑1 (MQ=255) | GATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGC‑GGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGG > KT2440/3440211‑3440468 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |