| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 690,162 | 0 | G | C | 53.3% | ‑0.6 / 14.5 | 15 | intergenic (+39/‑2420) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base G (5/2); new base C (8/0); total (13/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGGCCTCCCCG‑CTTTGTTTTTGGGGGTTGGTTGTTGC‑TGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAG > KT2440/690035‑690297 | gcTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGACGCCGCCCCC‑CCGTGtttt > 5:454836/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGTGGCCGGAGTGCCGGCCCGCCTAGGTTTTTGGGGggtggtggt > 5:384020/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCGGGCGCCCCC‑CGGTGGTTTTTGGGGGTGggtgtt > 5:310514/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGAGGCCGCCCCC‑CGATGTTTTTTGGGGTTTggtgtt > 5:199861/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGGCGGCAGGGCGCCCCC‑GGGTGTTTTTTGGGGGTGggtgtt > 8:194185/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCCGAAGGCCCCCCCG‑CTTTTTTTTTTGGGGTTGgttgtt > 2:287122/1‑137 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGCGGCCGGCCCCC‑CGTTGTTTTTTGGggttggggttgg > 3:65866/1‑131 (MQ=255) cccAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCGGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCAGCCGCCCCC‑CGTGGTTTTTGGGGGGTGGGTGGTGCGGGTTggggg > 8:464654/1‑136 (MQ=255) tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGCGGCCGCGCGCCCCCCCCC‑CGATGTTTTTTGGGGGTGGTTTTTGT‑TTTTGGGTGGGaaaggt > 5:233037/1‑133 (MQ=255) gCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCGGGCGCCCCC‑CGGTGGTTTTTGGGGGGGGTTGTTGG‑GGTTTGGGTGGGACTGttttttt > 3:249365/1‑134 (MQ=255) cccccG‑CTTTGTTTTTGGGGGTGGGTGGTTGC‑TGTGGGGGAGGAACTGTTTTGAGAGGGGGTGGGCGGGGAGAGGGGCCCCCCACAACCCTGGCAGTGCCGGTGAGACCGCCCGCCGCCCGCGCGGCGCTCGGtctc < 2:457402/136‑1 (MQ=18) cccG‑CTTTGTTTTTGGGGGTTGGTTGTTGC‑TGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTcac > 4:442242/1‑137 (MQ=255) cG‑CTTTGTTTTTGGGGGTTGGTTGTTGC‑TGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAAccc > 1:318077/1‑86 (MQ=255) cG‑CTTTGTTTTTGGGGGTTGGTTGTTGC‑TGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAAccc < 2:318077/86‑1 (MQ=255) cG‑CTTTGTTTTTGGGGGTTGGTTGTTGC‑TGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCCGCGCTCGGTCTCACAg > 8:380597/1‑137 (MQ=255) | GCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGGCCTCCCCG‑CTTTGTTTTTGGGGGTTGGTTGTTGC‑TGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCGCCACAACCCTTGCAGTGCCTGTGAGATCGAGCGCCGCCCGCGCGGCGCTCGGTCTCACAG > KT2440/690035‑690297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |