| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,503,734 | 0 | T | G | 51.8% | ‑2.6 / 10.1 | 27 | L36R (CTC→CGC) | CJ019_05179 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (8/5); new base G (7/7); total (15/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAAC > KT2440/5503613‑5503864 | tCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCACCCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCTCGCAGGCTTGgccgc > 4:268946/1‑137 (MQ=255)tCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTACTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCTGCCGGCCTTCTCGAAGGCTGGgcagc > 8:230566/1‑137 (MQ=255) gATGGCCCGCAGGCACCCCCTCCTGCCCACCCTCCTGCTGCTAAGCCCCCTGCAACCCCCCCCCCGCGAGGGCTAATTCTTTCACCCCGCCAAGCCCGCCGAGCAGGCCGGCCTGCGCGAAGGCTGGGCAGCACA‑Ac < 8:389075/137‑1 (MQ=255) gCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGCGCCGGCGGGCCGGCTCGAGGGCGGGGCCGCACA‑CCCCGAAGcc > 7:72990/1‑137 (MQ=255) gCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCCGCCCGCCAGCGCCAAAGCAGGGCCGCCCC‑Acccccagcc > 4:28245/1‑131 (MQ=255) gCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAACGCTGGGGCGCACA‑ACCCGATTcc > 7:457933/1‑137 (MQ=255) cTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGGTCGAAGGCGGGGGCGCCCACACCCCA‑GCCGCGCGCCGGCCcctggg > 4:121794/1‑135 (MQ=255) cTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGCTCCAACGCTGGGGCGCCCA‑AACCCCCACCGCGCGCCACCcgccgcg > 7:11528/1‑136 (MQ=255) tCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCCGCCTGCTCGAAGGCTGGGCACCACA‑ACCCGCTGCCGCGCGCCTGCCGctgctg > 6:325040/1‑137 (MQ=255) cctcctGCTGATAACCCCCCTGGCACCCCCGCCCTGCGTGGGCGATTTCTTTCTCACCCCCACCCCCGCCGAGCCGGCCGGCCTGCTCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGaaa < 4:281117/137‑1 (MQ=255) ctcctGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCCGCCGGCGGGCACGCGGGGCCTCACA‑ACCCCCTTCCCCCCCCCGGCCGCGGCTGGgaaa > 2:433956/1‑137 (MQ=255) cctGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGCCCCGCCTCCTCGACGCCGGGGCAGCCCA‑CACCCAGGCCGCCCCCCAGCCGCGGCGGGGAAAcc > 1:460019/1‑137 (MQ=255) tgctAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCTGCGCGCAGGCTGGGCAGCCCA‑ACCCCCACCCCCCCGCCAGCCCCCGCGGGCAAACCAgcgc > 5:154617/1‑137 (MQ=255) aTCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCCGCCGGGGCGCAGGCTGGGGCGCACC‑AACCGCTGCCGCCCGCCAGCCGCGGCTGGGAAACCAGCGCCacag > 4:430664/1‑134 (MQ=255) cAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCCGGCCGGCCGGGGGGAAGGGTGGGGCGCCCC‑AACCCATTCCCCGCGCCTGCCGCTGCGGGTAAACCAGCGCCCAGGCCGGATCGcc > 4:67199/1‑137 (MQ=255) accccACGCCCTCGAGGGCGAATTCTTTCTCACCGCCAACCCCGCCGAGCCGGCCGGCCTGCGCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGcca < 8:72990/135‑1 (MQ=255) cgCGGGGGCTAATTCTATCACCCCCCCCCGCCCGCGGCGCCGGCCGGCCTGCTCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACaa < 1:7715/136‑1 (MQ=255) ggtcGATTTCTTTCACCCCGCCAAGCCCGCCGCGCTGGGCGGCCGCGGCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCa < 2:276955/134‑1 (MQ=255) gggCGAATTCTTTCTCACCGCCAAGCCCGCCGAGGCGGCCCGCCCGGGCGCACGCGGGGCGGCCCC‑ACCCCCAGCCCCCCGCCGGCCCCGGGGGGGAACACAGCGCCCACGCCGCCGCGCCCCCAAAGACAAgcccc > 4:207905/1‑134 (MQ=255) aaTTCTTTCTCACCGCCAAGCCCGCCGAGCCCGCCGGCCTGCGCGCAGGCTGGGGAGCCCA‑ACCCCATGCCGCGCGCCTGCCGCTGCGTGAAAACCAGCGCCACGGCCGCATGGCCCACAATGACAAGCGCAAGGTg > 4:142594/1‑137 (MQ=255) ttctttctCCCCGCCACGCCCGCCGCGCCGGCGGGCCTGCGCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTgcg < 6:126471/137‑1 (MQ=255) ccccccGCCGACCCGGCCGGCCGCCGCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCt < 3:131932/134‑1 (MQ=255) cccccggccggccCGCGCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCt < 3:207905/132‑1 (MQ=255) ggccggccTGCTCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCg < 2:123464/137‑1 (MQ=255) ggccggccTGCTCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCg < 8:97552/137‑1 (MQ=255) gccTGCGCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAAc < 6:154617/137‑1 (MQ=255) ccgggCTCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAAc < 1:271535/133‑1 (MQ=255) | TCCAGGATTGCCCGCATGCTCAGACTCCTGCTCACCCTCCTGCTGCTAATCCCCCTGGCAACCCACGCCAGCGAGGGCGAATTCTTTCTCACCGCCAAGCCCGCCGAGCAGGCCGGCCTGCTCGAAGGCTGGGCAGCACA‑ACCCGATGCCGCGCGCCTGCCGCTGCTGGAAAACCTGCGCCAAGGCCGCATCGCCACCAATGACAAGCGCAAGGTGCGCCTGAACAACCGCCTGCGCGGCCTGATCGACAAC > KT2440/5503613‑5503864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |