| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 690,150 | T→G | intergenic (+27/‑2432) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 690,150 | 0 | T | G | 91.7% | 20.6 / ‑6.2 | 12 | intergenic (+27/‑2432) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (11/0); minor base C (1/0); total (12/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGGCCTCCCCG‑CTTTGTTTTTGGGGGTTGGTTGTTGC‑TGTTGGGGAGGAACTGTTTTGAG > KT2440/690021‑690211 | cccGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGCAGGccg > 5:309447/1‑136 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGCGGCcccccccgc > 8:202199/1‑134 (MQ=255) gcTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGACGCCGCCCCC‑CCGTGtttt > 5:454836/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGTGGCCGGAGTGCCGGCCCGCCTAGGTTTTTGGGGggtggtggt > 5:384020/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCGGGCGCCCCC‑CGGTGGTTTTTGGGGGTGggtgtt > 5:310514/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGCCGGGAGGCCGCCCCC‑CGATGTTTTTTGGGGTTTggtgtt > 5:199861/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGCGGGCGGCAGGGCGCCCCC‑GGGTGTTTTTTGGGGGTGggtgtt > 8:194185/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCCGAAGGCCCCCCCG‑CTTTTTTTTTTGGGGTTGgttgtt > 2:287122/1‑137 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGGGGCCGGCGGCCGGCCCCC‑CGTTGTTTTTTGGggttggggttgg > 3:65866/1‑131 (MQ=255) cccAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCGGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCAGCCGCCCCC‑CGTGGTTTTTGGGGGGTGGGTGGTGCGGGTTggggg > 8:464654/1‑136 (MQ=255) tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGGGGGCGGCCGCGCGCCCCCCCCC‑CGATGTTTTTTGGGGGTGGTTTTTGT‑TTTTGGGTGGGaaaggt > 5:233037/1‑133 (MQ=255) gCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGGGGGCGGGCGGGCGCCCCC‑CGGTGGTTTTTGGGGGGGGTTGTTGG‑GGTTTGGGTGGGACTGttttttt > 3:249365/1‑134 (MQ=255) | CCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGC‑GGGGAGGCCGTCAGGCCTCCCCG‑CTTTGTTTTTGGGGGTTGGTTGTTGC‑TGTTGGGGAGGAACTGTTTTGAG > KT2440/690021‑690211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |