Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 3,267,810 T→G 28.8% L171V (TTG→GTG)  eamA → putative amino‑acid metabolite efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,267,8100TG28.8% 23.5 / 4.5 26L171V (TTG→GTG) eamAputative amino‑acid metabolite efflux pump
Reads supporting (aligned to +/- strand):  ref base T (4/14);  new base G (6/2);  total (10/16)
Fisher's exact test for biased strand distribution p-value = 2.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGCGTA  >  KT2440/3267686‑3267937
                                                                                                                             |                                                                                                                               
gATGGGCAGCGAAGACGGCGGCCATGGGCCCATGCTGGGGTGGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTg                                                                                                                       <  2:136363/135‑1 (MQ=255)
  tCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTg                                                                                                                       <  1:169934/133‑1 (MQ=255)
  tCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTg                                                                                                                       <  3:272407/133‑1 (MQ=255)
   cGGCAGCGAAGACCGCGGCCATGTGCACATGATGGCGTTGGTGCTGACCCTGTGTGGCGGTCCGATGTGGTGCCAGGGCAACATCATTACCAGACGGTTCGGCTCGGTCGACCTGGTCGC‑CTTGCTGCTCCgggg                                                                                                                    >  3:81882/1‑135 (MQ=255)
    ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACCTCATTACCCGACGGTTCGGCTCGGTCGCCCTGGTCGC‑GGTGGGGGTCTGGGGt                                                                                                                   >  7:2234/1‑135 (MQ=255)
    ggCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTGGGTGAGCTGGGGt                                                                                                                   >  8:17625/1‑135 (MQ=255)
         cGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GGTGGTGGTGTGGGGTGTGCt                                                                                                              >  6:37866/1‑135 (MQ=255)
         cGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGGGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCt                                                                                                              <  8:216370/135‑1 (MQ=255)
                                       gTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTc                                                                                <  5:20585/135‑1 (MQ=255)
                                                        gtgGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCAGGGGTGGGGTGATTCCGCCACATACATTCATGGCCCTGTGCTGGTGGCTGGAAGGcc                                                               >  8:7856/1‑135 (MQ=255)
                                                              gTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgc                                                         <  1:120829/135‑1 (MQ=255)
                                                              gTGCGATGGGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgc                                                         <  6:3652/135‑1 (MQ=255)
                                                              agacagtgtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAgc                                                         <  3:140479/129‑1 (MQ=255)
                                                                    tgtgGGGCCTGGGCAACATCATTCCCCGACGGTTCGGCGCGGTCGACCTGGTCGG‑GTTGGTGATCTGGGGTGGGCTGATACCGGCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTg                                                   <  1:134431/135‑1 (MQ=255)
                                                                    ggtgGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTg                                                   <  2:269870/134‑1 (MQ=255)
                                                                         ggCCGGGGCAACATCATTACCCGACGGTTCGGCGCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCAt                                              <  4:165683/135‑1 (MQ=255)
                                                                                gCAACATCATTACCCGAGGGTGCGGCGCGGTCGACCTGGGCGC‑GTTGGTGTTCTGGGGTGGGCTGATACCGGCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTgg                                       <  4:81882/135‑1 (MQ=255)
                                                                                  aaCATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCGGATACCGCCACTTACATTCCTGGCACTGTCCTGGTGGCTGGCAGGCCCCCAGCGCATTGGCCATGCCCTgtct                                     >  4:140479/1‑132 (MQ=255)
                                                                                   aCATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GGTGGTGATCTGGGGTGGGCTGATTACCCCCCATAGCTTGCTGGCACTGTCCTGGTGGCTGGTAGGCCCCGAGCGCATTGGCCATGCCATgggc                                     >  3:108794/1‑132 (MQ=255)
                                                                                                  ggTTCGGCTCGGTCGACCTGGTCGC‑GGTGGTGTTGTGGGGTGGGGTGATACCGCCACTTGCATTCCGGGCACGGTCCGGGTGGCTGGAAGGCCCCGAGCGCAATGGGCCTGCCTTGGGCAATATCAGTTgggg                       >  7:124258/1‑131 (MQ=255)
                                                                                                    ttCGGCTCGGTCGACCTGGTCGCGGGTGGGGTTATGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTTTCCTGGTGGGGGGGAGGCCCCGAGCGCATTTGCCATGCCTTGGGCAATATCAGTTGGCGttg                    >  8:123030/1‑134 (MQ=255)
                                                                                                    ttCGGCTCGGTCGACCTGGTCGC‑GGTTGGGTTCTGGGGTGGGGGGGTACCGCCCCATCCATTCCTGGCCCTGTCCTGGTGGGTGGAAGGCCCCCAGCGCATTGGCCATGGCATGGGCAATATAAGTTGGAGTTCg                   >  4:297918/1‑135 (MQ=255)
                                                                                                              tCGAGCTGGTCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc         <  3:219000/135‑1 (MQ=255)
                                                                                                              tCGACCTGGTCGC‑GGTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc         <  6:228957/135‑1 (MQ=255)
                                                                                                              tCGACCTGGTCGC‑GGTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGccc         <  7:133725/135‑1 (MQ=255)
                                                                                                                       tCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGCGTa  <  1:22737/133‑1 (MQ=255)
                                                                                                                             |                                                                                                                               
GATCGGCAGCGAAGACGGCGGCCATGTGCCCATGCTGGCGTTGGTGCTGACCCTGTGTGGCGGTGCGATGTGGGGCCTGGGCAACATCATTACCCGACGGTTCGGCTCGGTCGACCTGGTCGC‑GTTGGTGATCTGGGGTGGGCTGATACCGCCACTTCCATTCCTGGCACTGTCCTGGTGGCTGGAAGGCCCCGAGCGCATTGGCCATGCCTTGGCCAATATCAGTTGGAGTTCGGTGCTGGCCCTGGCGTA  >  KT2440/3267686‑3267937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑