| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,145,586 | A→C | 24.1% | intergenic (+131/+20) | CJ019_03903 → / ← nicP_9 | hypothetical protein/Porin‑like protein NicP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,145,586 | 0 | A | C | 24.1% | 59.7 / 3.5 | 48 | intergenic (+131/+20) | CJ019_03903/nicP_9 | hypothetical protein/Porin‑like protein NicP |
| Reads supporting (aligned to +/- strand): ref base A (24/11); new base C (3/9); total (27/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGCCGGACC > KT2440/4145463‑4145716 | tGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGGCGGGGTGAGAAGGGCGGc‑cccg > 5:82519/1‑133 (MQ=255)tGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAAGGCCGGC‑CCGt > 3:99898/1‑135 (MQ=255)tGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCGTCAGAAGGCCGGC‑CCGt > 8:136810/1‑135 (MQ=255)tGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCCGCGTCAGAAGGCCGGC‑CCgg > 7:68434/1‑134 (MQ=255) tttCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAAGGCCGGC‑CCGTTTCg > 8:83737/1‑134 (MQ=255) tttCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAAGGCCGGC‑CCGTTTCg > 2:30843/1‑134 (MQ=255) tttCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAAGGCCGGC‑CCGTTTCg > 3:146777/1‑134 (MQ=255) tttCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCCGCGTGAGCACGCCGGCGCCGttagc > 8:261201/1‑132 (MQ=255) tttCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCCGCGTCAGAAGGCCGGG‑CCGgttgg > 7:261944/1‑132 (MQ=255) tttGAAAAAGAGCAACGGGCTGGGGGCAGGGGCCCGACCGCCGGGTGAAATTCAGGCGAAAACCAGCCAACCCTTCGATTGGC‑AAAAAATGGCCAAAAATGGGCCGGCTTCATACGCCCGGC‑CCGTTTCGCTAtt < 4:149259/134‑1 (MQ=255) gTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCCGCAGGCCGGC‑CCGTGTCGcgtgt > 7:151313/1‑131 (MQ=255) ttGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCCGAAAGCCCGC‑CCCTTTCGCTAttt > 2:157100/1‑135 (MQ=255) ttGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGACGGCCGGC‑CCGGTTCGCGAttt > 4:84197/1‑135 (MQ=255) ttGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAATGCCGGC‑CCGTTTCGCtttt > 4:73196/1‑134 (MQ=255) ttGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCGTCAGAAGGGCGGC‑CCCGTTCgggaatt > 4:287912/1‑129 (MQ=255) ttGCAAAAGATGAAACGGCTGGGTGCAGGCGTCAGAACGCCTGGTGCAATTCAGGCGGAAAAAAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTGAAGACTGCCGGC‑CCGTGTCGcaagtt > 7:155527/1‑130 (MQ=255) aaaGATGAAACGGCTCGGAGCAGGCGTCAGAACGCCTGTTGCAATTCAGGCGGAAAACAGCGAACCCATCGATTGGG‑AATAAATGGCGAAAAATTCGCCCGCATCAGACAGCCCGC‑CCCGTTGGCTATTTAGaaa > 2:159018/1‑134 (MQ=255) aaacgggCTGGGTGCTGGGGTCGCAACGCTGGGTGCATTTCAGGGTTAAAACACCCACCCCTTCTTTGGGG‑AATAAATGGCGAAAATTGGGCCGGCTTCATAAGGCGGGC‑CCGTTTCGCTATTTAGAAGTTGAAg < 6:82519/130‑1 (MQ=255) aacgggCTGGGGGCCGGCGTCCCACGGCCGGGGCCAATACCGGCGAAAACCCGC‑CCCCCATCGCTTGGGAAAAAAAGGGCAAAAAAGGGCCCGGCTCACCAGGGCGGGC‑CCGTTTCGCTATTTAGAAGTTGAAg < 6:243492/130‑1 (MQ=255) aaaCGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAgg > 2:14534/1‑135 (MQ=255) gaggggTGCAGGCGGCCGAACGCCGGTTGAAATTCCGGCGTAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGGTGCTGACGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAg < 8:167878/132‑1 (MQ=255) cGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGAt < 4:146777/135‑1 (MQ=255) aaCGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCGTCAGCAGGCCGGC‑CCGGTTCGCGATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGAtt > 4:72757/1‑134 (MQ=255) aCGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCATAAGGCCGGC‑CCGGTTAGCAATTTATAAGTTGAAGGTGTAGCGCATGATCAGGCGAttt > 6:215295/1‑134 (MQ=255) aCGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCGTACGCACGCCGGC‑CCCGTTCGCGATTTAGAAGTTGAAGGTGTAGCGCATGTACAGGCGAatt > 7:199566/1‑134 (MQ=255) aCGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCATCAGCACGCCGGC‑CCGTTTCGGTATTTAGAAGTTGAAGGTGTAACGCATGAAGAGGCGAtgt > 4:165466/1‑132 (MQ=255) tGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATTGGCCGGCTTCAGAACGCCGGC‑CCGGTTCGCTATTTAGAACTTGAAGGTGTATCGCATGAACAGGCGAATTTAATTa > 4:113519/1‑135 (MQ=255) tGCAATTCCGGCGCAAAACCGCAACCCCTGCGTTGGGC‑AAAAAAGGGCCAAAACGGGGCCGGCTTCAGAAGCCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAgcg < 1:162813/135‑1 (MQ=255) ggCAATTCCGGCGCAAAACCGCGAACCCTTCGATTGGC‑AAAAAATGGCTAAAAATGGGCGGGCTTCAGACGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAgcg < 5:126888/134‑1 (MQ=255) ggCAATGCAGGCGGAAACCAGCCAACCCTTCGATTGGG‑AATAAATGGCGAAAAAGGGGCCGGCTTCACAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAg < 4:206098/132‑1 (MQ=255) gCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAgcgc < 4:99898/135‑1 (MQ=255) gCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCCTCAGAAGGCCGGC‑CCGTTTCGCTATTTATAAGTTGAACGTGTAGCGCCTGCTCAGGCGAGTTTCATTAgcgc > 6:77481/1‑135 (MQ=255) aGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTAAGAAGGCCGGC‑CCGGTTCGCTATTTTGAATTTGAAGGTGTAGCGCATGATAACGCGAATTTCATTAGCGCTGTCAGc > 2:204875/1‑135 (MQ=255) aaCAGAGAACCCTACGATTGTG‑AATAAATGGCTAAAATTGTCCCGGCTTCATACGGCGGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCg < 8:53294/135‑1 (MQ=255) accgCGACCCCTTGGATTGGT‑AAAAAATGGCTAAAAATGGGCCGGCTTCAGACGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGa < 8:261944/132‑1 (MQ=255) caccccGTCGATTGGG‑AATAAATGTCGAAAAATGGCCCGGCTGAAGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGt < 5:43855/132‑1 (MQ=255) aaCCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCGTCAGAAGGCCGGC‑CCGGTTGGCGTTTTAGAAGTTGAAGGTGTTGCGCATGATCAGGCGCTTTTAATTATCGCTGTGAGCGTTGGTCGAGCGGtt > 3:52468/1‑135 (MQ=255) gATTGGG‑AATAAATGGCGAAAAAGGGGCGGGCTTCAGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAg < 3:266421/134‑1 (MQ=255) g‑AATAAATGGCGAAACATGGGCCGGCTTCAGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGc < 4:202901/134‑1 (MQ=255) aaaTGGCGAAAAATGGGGCGGCTTCAGCAGGCCGGC‑CCGTTTGGCGATTTAGAAGTTGAAGGTGTTGCGCATGATCAGGCGATTTTGATTAGGGCTGTGAGCGTTGGTCGCGCGGTTCGTGGTGTTACGCCCCt > 6:154184/1‑134 (MQ=255) aaaTGGCGAAAAATGGGCCGGCTTGAGAAGGCCGGC‑CCGGTTCGGTATTTATAAGTTGTATGTGTAGCGCATGTGCCGGCGATTTTAATTTTCGCTGTCCGCGTAGGTCGCGCGGTTCGTGGCGTTTCGCCCCt > 7:235659/1‑134 (MQ=255) ggcaaaaaTGGGCGGCCTTCTGACCGCCGGC‑GCGTTTCGCTTTTTAGAAGTTGAAGGTGTAGCGCATGATCTGCAGATTTTCAATAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTgaaacg < 3:240701/132‑1 (MQ=255) caaaaaGGGGCCGGCTGCACACGGCGGGC‑CCGGTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTgaaacgaa < 1:247176/134‑1 (MQ=255) aaatTGGGCGGGCTGCAGACGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGGCGAGCGGTTCGTGGAGTTACGCCACTgaaacgaaac < 3:176961/135‑1 (MQ=255) ggattCAGACGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGcc < 7:66125/132‑1 (MQ=255) ggCTTCAGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGcc < 4:54196/135‑1 (MQ=255) aGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGCCGGAcc < 7:166421/134‑1 (MQ=255) aGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGCCGGAcc < 1:30843/134‑1 (MQ=255) | TGGCCTTTCGTTGCAAAAGATGAAACGGCTGGGTGCAGGCGTCCGAACGCCTGGTGCAATTCAGGCGGAAAACAGCGAACCCTTCGATTGGG‑AATAAATGGCGAAAAATGGGCCGGCTTCAGAAGGCCGGC‑CCGTTTCGCTATTTAGAAGTTGAAGGTGTAGCGCATGATCAGGCGATTTTCATTAGCGCTGTCAGCGTAGGTCGAGCGGTTCGTGGAGTTACGCCACTGAAACGAAACACCCTTCGCCGGACC > KT2440/4145463‑4145716 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |