| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,258,563 | A→C | 24.4% | D44A (GAC→GCC) | pht2 → | Phthalate 4,5‑dioxygenase oxygenase reductasesubunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,258,563 | 0 | A | C | 24.4% | 122.5 / 4.0 | 84 | D44A (GAC→GCC) | pht2 | Phthalate 4,5‑dioxygenase oxygenase reductasesubunit |
| Reads supporting (aligned to +/- strand): ref base A (15/45); new base C (22/2); total (37/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.62e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCG > KT2440/4258429‑4258695 | cGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCAGCCCGa > 1:1775/1‑135 (MQ=255)cGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTAAGCGCAGGCGCCCATATCGCCGT‑GCCCCTGCCCGa > 4:38004/1‑135 (MQ=255) gACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCACCTGCCCGa > 8:74188/1‑135 (MQ=255) aCATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACg < 6:51682/135‑1 (MQ=255) aTCGATGGCGTAGTGGGATCCCGTAACGATGAAGCGCAGGGTACGTCCAGCTTCGAGCTGGCCGCGGCTGATGGCAGCCTGCTGCCGGCGCTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTg < 2:74756/135‑1 (MQ=255) cGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGt < 5:228299/135‑1 (MQ=255) aTGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTgc < 5:7300/135‑1 (MQ=255) ttAGTGGTATCCCGTACCGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATAGACGT‑GGACCAGCCCGAACGGCAGGTTCG‑CCa > 6:114620/2‑134 (MQ=255) ttgTGGTATCCCGTAACGATCACGCGCAGGGTATCTGCTGCTTCGAGCTGGCCGCGGCAGAGGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGt < 7:167112/133‑1 (MQ=255) tAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGt < 5:300062/135‑1 (MQ=255) tAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGt < 3:70593/135‑1 (MQ=255) tAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGt < 3:149465/135‑1 (MQ=255) tGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTAt < 2:258372/134‑1 (MQ=255) tGGGATCCCGTACCGCTGAAGCGCAGGGGAGCTGCAGCTTCGAGGTGGCCGCGGCAGATGGCAGCCTGCGGCCGGCGGTCAGCGCCGGCGCCCATATCGACGG‑GCCCCTGCCCGACGGGCTGGTGCG‑CCAGTAt < 8:64591/134‑1 (MQ=255) gTAGCCCGTAACGATGAAGCGCAGGGTATCTGCAGCGTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCg < 8:145082/135‑1 (MQ=255) tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GGCCCGGCCCCACCGGCAGGGGGG‑CCAGGAttacgag > 7:173589/1‑130 (MQ=255) ccGTAACGTTGAAGCGCAGGTTAGCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCGCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCtgtg < 2:267378/135‑1 (MQ=255) ccGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGACCGCCGCGCTGGTGCG‑CCAGTATTAGCtgt > 6:104789/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCCGCCTGCTGCCGGCGTTCAGCTCCGGCGCCCAAATCGACGA‑GCCCCGAGCCCACGGGCGGGTGCG‑CCCTTATTAGCtttg > 8:82507/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GGCCCCGCCCCCCCGGCGGGGGCG‑CCCGGGTTCGCggtgg > 2:268315/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCGCCATCCCGCCGGGCTGGTGCG‑CCAGTATTCGCTGTGc > 3:46338/1‑135 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGCCGGGCTGGTGCG‑CCAGTATTAGCtgtg > 2:56185/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGCCCGGCGGGGGCG‑CCCGTATTCGCtgtg > 5:157581/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGTCTGGTGCG‑CCAGTATTCGCtgtg > 7:31579/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACCGGCTGGTGCG‑CCAGTATTCGCtgtg > 6:129967/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCGGCCCGCCCGGCTGGTGCG‑CCCGTATTCGCTGTGc > 3:263866/1‑135 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCGGCCCCACCGGCGGGTGCG‑CCAGTATTCGCtgtg > 3:267514/1‑134 (MQ=255) cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCTGCCCGCCCGGCGGGTGCG‑CCAGTATTCGCGGTGCAaccc > 7:296648/1‑134 (MQ=255) cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCAGCCCCCCGGGCCGGTGCG‑CCACTATTAGCTTGGCAacca > 4:183682/1‑135 (MQ=255) gAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCAcccc < 8:254016/135‑1 (MQ=255) aaGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCCACGGGCGGGTGCG‑CCAGTATTAGCAGTGCAACcccccc > 6:290679/1‑134 (MQ=255) aGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCAGCCCCCCGGGCTGGTGGG‑CCACTATTAGCGGTGCACCCCCCCCgc > 7:84795/1‑134 (MQ=255) gcgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCCGCCCGCCCGGGGGGGGCG‑GCAGTATTCGCGGTGCAACcccccc > 6:243009/1‑132 (MQ=255) cgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GGGCCGGCCCCCCCGGCGGGGGGG‑CCAGTATTTGGTGTTGAACcccccccca > 6:171445/1‑131 (MQ=255) gcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCGGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCgaaga < 2:175908/135‑1 (MQ=255) aTCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGCAGGGCTGGTGCG‑CCAGTATTAGCTGTGCCACCCCCCCGAACAACGCCAt > 4:294816/1‑135 (MQ=255) ctgctgCGTCGAGCTGGCCGCGGCAGATGGCTGCCTGCTGCCGGCGATCAGCGCCGGAGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATc < 1:120772/134‑1 (MQ=255) cAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCAGCCCGACGGGCGGGTGCG‑CCAGTATTGCCTGTGGAACCACCCCCCAGAACGCCCTcccg > 3:295637/1‑131 (MQ=255) aGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCg < 5:276298/131‑1 (MQ=255) gCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATc < 3:295378/135‑1 (MQ=255) gCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATc < 3:82436/135‑1 (MQ=255) tCGAGCTGGGCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGa < 2:29223/135‑1 (MQ=255) tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCCGCGCCGGCGCCCATATCGACGT‑GGCCCAGCCCCCCCGGCGGGTTGG‑CCAGGTTTGGCTGTGGCACCCCCCCGCAGCACCCCCTCGCGATCTGa > 2:113748/1‑135 (MQ=255) tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCTGCCCGCCGGGCGGGTGGG‑CCAGTATTAGCTGTGCAACCCCCCCGAAGCACGCCATCGCGATCTGa > 4:267343/1‑135 (MQ=255) tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCCTGCCGACGGGGTGGTGGG‑CCAGTATTAGCTGTGCCAACCCCCCCAAGAAAGCCCTCGCGATCTGa > 7:32664/1‑135 (MQ=255) tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCGGCCCGCCGGGCTGGTGCG‑GCAGGATTCGCTGTGCCACCACCCCCGAGAACGCCATCGCGATCTgg > 4:119270/1‑134 (MQ=255) tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑CGAACAGCCCGCCCGGCTGCTGCGCCCCGTATGC‑CTGTGCAACCACCCCGCACAACCCCCTAGCTATcggt > 4:38085/1‑132 (MQ=255) cGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCGGGCCGCCGGGCTGGGGGG‑CCCGGTTTCGCTGTGGAACCACCCCCAAGCACGCCCTCGCGGTCTGAt > 5:142173/1‑135 (MQ=255) cGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGCCGGGCTGGTGCG‑CCAGGATTCGCTGTGCCACCACCCCGAAGAACGCCCTCGCGATCTGAt > 7:232646/1‑135 (MQ=255) gAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGa < 5:129967/133‑1 (MQ=255) aGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCTGCCCGCCGGGCTGGTGGG‑GCAGTATTAGCTGTGCAACCCCCCCGAAGAACGCCCTCGCTTTCTGATTg > 4:228779/1‑135 (MQ=255) aGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTg < 7:185762/135‑1 (MQ=255) cgggCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGc < 7:265079/133‑1 (MQ=255) gcgGCAGATGGCAGCCTGCTGCCGGCGTTCCGCGCCGGCGCCCATATCGACGT‑GGACCTGCCCGACGGGGTGGTGCG‑CCAGGATTCGGTGTGGAACCACCCCGAAGAACGCCAACGCGATCTGATTTGCGTAca > 1:187964/1‑133 (MQ=255) gcgGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCCCCTGCCCGCCGGGCTGGTGCG‑GCAGTATTCGCGGTGCAACCCCCCCGGAGAAAGCCATCGCGATCTGATTGGCGTTCt > 6:117250/1‑134 (MQ=255) tGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGAcc < 6:92727/134‑1 (MQ=255) aGCCTGCGGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGCCGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCt < 3:61710/135‑1 (MQ=255) tgctgcCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTcgc < 3:142771/135‑1 (MQ=255) tgacGGCGTTCAGCGCCGGCGCCCTTATCGACGG‑GCACGTGCCCGACGGGCTGGTGCG‑CCTGTATTCGCTGGGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTAGTCAACGACCCGGCTTCGCgg < 8:161638/131‑1 (MQ=255) ccGGCGTTCCGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGCCGGGCTGCTGGG‑CCAGGATTCGCTGTGCCACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGc > 4:24666/1‑135 (MQ=255) cGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 6:126080/133‑1 (MQ=255) cGGCGTGCCGCGCCGGCGCCCATATCGACGT‑GCCCCGGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 3:105791/133‑1 (MQ=255) cGGAGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 5:104789/133‑1 (MQ=255) cAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCg < 7:224410/135‑1 (MQ=255) cgcgcGGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCg < 2:139331/133‑1 (MQ=255) aGCGCCGGCGCCCATCTCGCGGT‑GCACCTGCCCGACGGGGTGGGGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATTTGTTTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGt < 5:243996/135‑1 (MQ=255) gggCGCCGATAGCGACGT‑GCACCTGCCCGACGGTGTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCTTCGGTTTCTGATTGGGGTACTCAACGACCCGGCTTGGCGGGGCGGTTCTCGTAGCCt < 4:158145/134‑1 (MQ=255) gcgcCCATATCGACGT‑GGCCCAGCCCCACGGGGTGGGGGG‑GCCGGATTCGCGGTGGACCCCCCCCCCAGAACCCCAGCGCTCTCGGGTTGGCGTACTCCACGCCCCGGCGTCGCGGGGCGGTGGTCGTCGccggg > 8:120126/1‑132 (MQ=255) tcccATTTCGACGT‑GCACCGGCCCGACGGGATGGTGCG‑CCAGTATTCGCTGTGCAACCTCCCCTAAGAACGCCATCGCTATCTGATTGGCGTTCTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc < 7:70704/134‑1 (MQ=255) gcCCATATCGACGG‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc < 3:38004/135‑1 (MQ=255) gcCCACAACGCCGC‑GCACCTGCCCGCCGGGCGGGTGCG‑CCAGTAATCGCTGTGCAACCACCCCGAAGCACGCCATCGCTATCTCATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc < 1:273525/135‑1 (MQ=255) cccATATCGCCGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACg < 1:233213/135‑1 (MQ=255) cccATAGCGACGG‑GCACCTGCCCGACGGGCGGGTGCG‑CCATTATTCGCTGTGCAACCACCCCCAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACg < 6:160488/135‑1 (MQ=255) atCGACGT‑GCCCCGGGCCCACCGGGGGGTGCG‑CCAGTATTCGCTGTGCAACCCCCCCCCACAACGCCCACGCGATCTGATTGTCGGACACAACGCACCCGCGTCGCGGGGCGGTTAGGGTAGCCGGCACGAACAg > 4:127708/1‑135 (MQ=255) cGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCa < 1:56185/135‑1 (MQ=255) cGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCa < 4:136614/135‑1 (MQ=255) cGT‑GCACCTGCCCGACGGGCGGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCa < 5:243009/135‑1 (MQ=255) cTGCCCGCCGGGCTGGGGCG‑CCAGTATTCGCTGTGCAGCCCCCCCCCAGAACGCCCTCGCTATCTGATTGGCGTGCTAAACGACCCCGCGTCGCGGGGCGGGTGGCGTAGCCTGCACGCACAGGTGCAAGCCGGt > 4:239805/1‑135 (MQ=255) cccGAGGGGCGGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCGTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGcc < 4:1214/135‑1 (MQ=255) cccGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGcc < 7:277577/135‑1 (MQ=255) cccGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGcc < 4:108086/135‑1 (MQ=255) cccGACGGGCTGGTGCG‑CCAGAATGCGCTGTCCAACCCCCCATAAGAACGCAATCGCTATCTGATTGGCGAACACAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGcc < 1:136937/135‑1 (MQ=255) ccGACGGGCTGGTGAG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGcc < 7:74188/134‑1 (MQ=255) cGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCgaaga > 8:271609/1‑45 (MQ=255) cGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCgaaga < 7:271609/45‑1 (MQ=255) cGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCg > 4:47070/1‑135 (MQ=255) cGACGGGCTGGTGCG‑CCAGTATTAGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCAGATTGTCGTACTCAAACACCCGGCTTCGCGGGGCGGTTCTCCTAGCCAGCACGAACATGTTCAAGCCGGTGcccc > 4:257771/1‑134 (MQ=255) | CGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGT‑GCACCTGCCCGACGGGCTGGTGCG‑CCAGTATTCGCTGTGCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGCCGGTGCCCG > KT2440/4258429‑4258695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |