| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,789,443 | A→C | 31.1% | V230G (GTG→GGG) | dat ← | Diaminobutyrate‑‑2‑oxoglutarateaminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,789,443 | 0 | A | C | 31.1% | 42.7 / 6.9 | 53 | V230G (GTG→GGG) | dat | Diaminobutyrate‑‑2‑oxoglutarateaminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (21/13); new base C (13/4); total (35/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
CGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCA > KT2440/4789318‑4789575 | cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCTCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGAAACAC‑TTCGATGATCACGGCGGCGGGCAGCGGCACCCCGCctt > 4:265460/1‑135 (MQ=255)cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCttt > 6:29932/1‑135 (MQ=255)cgacgaTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCgtt > 5:129597/1‑135 (MQ=255) aTCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑ATCGAGGATCACGGCGGCGGGCACCGCCACGCCGCTTTACGGa > 3:137231/1‑135 (MQ=255) acgccacgccCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGGAGCGGCCCGCCGCCTGCCCGATCgc > 8:268268/1‑134 (MQ=255) ccgccTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGt > 8:106044/1‑135 (MQ=255) ggTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGAGCAGGAGGTTTTCCAGGTa > 3:61464/1‑135 (MQ=255) tAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCCCCCTGCACCAC‑TTCGAGGAGCACGGCGGCGGGCCGCCGCCCGCCGCTGTCCGGATCGCGCCGCCTGGTTTGCAGGGAAt > 6:192000/1‑135 (MQ=255) tGCGCCGCACGCCTTTGAGCCATGCGATGTGGCCCGTAACCACCCCGCCCACCCCCTCCCCCAA‑TTCGAGCACCAGGGCGGGGGGCAGCGCCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa < 6:132047/135‑1 (MQ=255) tGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCCCCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCa < 7:75638/135‑1 (MQ=255) cgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTg > 1:162515/1‑135 (MQ=255) cgcACGCCCTTGAGCCATTCGATGTCGGCCGGAACCA‑CCCGCCCTCACCCTGCACCAC‑TTCGAGGCTCACGGCGGCGGGCCGCGGGCCGCCGCGTTCCGGATCGCGCCGCAGGGTTTGCAGGTGATGGAGGTTgt > 5:194142/1‑134 (MQ=255) ccACGCCCTTGAGCCATTCGATGGCGGCCGCAACCACCCCCCCCTCCCCCGCCACCAC‑TGCGAGCACCCCGGCGGGGGGCCGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTgg < 2:162515/134‑1 (MQ=255) aCGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCCGCATGCCGCCTTCCGGATCGAGCCCGCGGTGTTCCAGGGAAATCATGTTgggc > 1:267668/1‑133 (MQ=255) gCCCTTGAGCCATTCGATGTCGGCCGGAACCCCCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCCGCCCGCCCCGTACCGGTTAGAGCAGCACGGTTTACACGTATTGCAGGTTgggcga > 5:246209/1‑131 (MQ=255) tGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCGTTCCGGGTCGAGCAGCCGGTTTTCCAGGTAATGCAGGTTTGCCGTG‑Accc > 4:264717/1‑135 (MQ=255) cccccttCGAGGGCGGCCGCACCCCCCCCGCCCGCCCCCTGCACCAC‑TGCGAGGACCCCGGCGGCGGGCAGCGCCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑Accc < 7:70113/128‑1 (MQ=255) ccATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCCGCACACCCCATTACGGTTCGCGCAGCATCTTTTCCAGGTAATGCAGGATTGCCTTGTACCCCgc > 4:204066/1‑134 (MQ=255) tttCGAGGTCGCCCGCAACCCCCCCGCCCTCCCCCTGCACAAG‑TGCGAGGACACCGGCGGCGGCCTGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCt < 2:212785/134‑1 (MQ=255) aTTCGAGGGCGGCCGCACCCACCCC‑CCCGCCCCCCGGCCCACGTTCGAGCAGCCGGGCGCCGGGCCGGGCCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCt < 3:36767/135‑1 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGCGCCCCCCCGGTGCCGCGCCAGCAGCCCGTGTTACACGTAATGCAGCTTGGCCTTT‑ACCCCCGCCCCAcc > 6:174963/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCCCGGCCCGCCCCCTTACGGCTAGCGCACCAGGGTTTGAAGGTTATGCAGGTTTGCCGTG‑ACCCCGcccgcccc > 2:228841/1‑130 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGCTCGAGCAGCAGGGTTTCCAGGTAATGCAGGTTGGCCTTT‑ACCCCGGCCTCAcc > 8:248505/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCTTTCCGGATCGCGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCCGCCTcccc > 6:140482/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCCCGCCGCGTTCCGGATCGAGCAGCAGGTTTTGCAGGTAATGCAGGTTGGGCTTG‑ACCCCGGccgcccc > 3:19592/1‑130 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGGTTTGCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCAcc > 8:249684/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGCGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCAcc > 7:228854/1‑135 (MQ=255) gATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCCGCACGCCGCCTTGCGGATCGAGCAGCAGGTTTTACAGGTAATGCAGGTTGGGCATT‑ACCCCCGCCTAAcc > 7:184959/1‑135 (MQ=255) ggAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGGCGCGCCGCGGTGCGGCGCGCGCCGCCGGGTTTGCAGGTTATGCAGGGTGGGCGTG‑GCCCCCGCCGCCCCCCCCCGGccc > 2:128526/1‑134 (MQ=255) ggAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCGTTCCGGGTCGAGCAGCAGGTTTTCCAGGGAATTGAGGTTGGGCTTG‑ACCCCGGCCTCACCCCCCAGGccc > 8:13302/1‑134 (MQ=255) cGCCCACACCCTCCACCCT‑TGCGTGCATCACGGGGGCGGGCCGCGGCCCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGc < 8:282734/135‑1 (MQ=255) gCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATAGAGCAGCAGGGTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGCCCACACCCCCCAGGCCCAACGGGCAGCg > 7:282734/1‑135 (MQ=255) ccccgccaccag‑TTCGCGGTACCCGGCGGCGGGCTGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTc < 4:135511/129‑1 (MQ=255) cTGCACCAC‑TTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCTTTCCGGGTCGCGCAGCAGGTTTTCCAGGTTATGCCGGGTGGGCGTG‑ACCCCGGCCTCACCCCCCCGGCCGACCCGGCCGCGGTAGTCGTa > 5:19902/1‑135 (MQ=255) accac‑TTCGAGGATCACGGCGGCGGGCCGCGGCCCGCCGCGTTGCGGATCGCGCAGCAGGTTTTGCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCGCCCCGCCCACGCCGCACGGGCCGCGGGAGGCGGACggg > 2:95773/1‑135 (MQ=255) accctttCGAGGATCCCGGCGGGGGGCAGCGGCCGCCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACg < 7:192581/128‑1 (MQ=255) cac‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACggg > 8:154004/1‑133 (MQ=255) cac‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCGTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTTGCCTTG‑ACCCCCGCCTCACCGCCCCGGCCGCACCGGCAGCGGTAGTGGTACggg > 3:14162/1‑133 (MQ=255) ac‑TTCGAGGAGCCCGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTaa < 6:129597/135‑1 (MQ=255) tCGAGGATCACGGCGGCGGGGAGCGGCACCCCGCTTTACGCATAGAGCACCAGGTTTTCCAGGTAATGCAAGTTGGCATTT‑AAACCCGCCACACCCCCCAGGCCGAACCGGCAGCGGTAGACGTACGGGTAAgcc > 6:95773/1‑133 (MQ=255) cGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCATG‑ACCCCGGCCTCACCGCCCAGGCCGAAAGGGCAGCGGTAGTCGTACGGGGAAGGCa > 3:54438/1‑135 (MQ=255) gAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCCGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCCCCCTCCCCGCCCAGGCCGCACCGGCAGCGGTAGTCGTACGGGTAAgg > 3:39401/1‑132 (MQ=255) ctgaaTCCCGGGGGGGGGCTGCGGAAGGCGGATTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCAt < 1:86208/131‑1 (MQ=255) ggATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGa > 7:3361/1‑135 (MQ=255) gcgcccggcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGGTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGaa < 7:149192/130‑1 (MQ=255) tCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACt < 7:106044/135‑1 (MQ=255) cggccgcggGCACCGCCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAAGGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCa < 2:252966/135‑1 (MQ=255) ggcggcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCAc > 7:29592/1‑135 (MQ=255) gcggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCg < 4:145767/135‑1 (MQ=255) cggGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGt > 4:107120/1‑135 (MQ=255) gggCCGCGGCTCGCGGCTTTCCGGATCGAGCAGGAGGTGTTCCAGGTAATGCAGGTTGGCGTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGGGGTAGTCGTACGGGTAATGCATGAACTGCACGCCGtt < 7:11428/135‑1 (MQ=255) gggCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGtt < 3:153990/135‑1 (MQ=255) gaaCGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCa < 1:193941/133‑1 (MQ=255) gCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCa < 5:151899/135‑1 (MQ=255) | CGACGATCAACGCCACGCCCGCCTGCTCGGTAATGCGCCGCACGCCCTTGAGCCATTCGATGTCGGCCGGAACCACCCCGCCCTCACCCTGCACCAC‑TTCGAGGATCACGGCGGCGGGCAGCGGCACGCCGCTTTCCGGATCGAGCAGCAGGTTTTCCAGGTAATGCAGGTTGGCCTTG‑ACCCCGGCCTCACCGCCCAGGCCGAACGGGCAGCGGTAGTCGTACGGGTAAGGCATGAACTGCACGCCGTTGCCGAGCA > KT2440/4789318‑4789575 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |