| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,255,897 | 0 | A | C | 52.9% | ‑0.0 / 10.6 | 18 | intergenic (‑152/+72) | pnuC/mcpP | Nicotinamide riboside transporter PnuC/Methyl‑accepting chemotaxis protein McpP |
| Reads supporting (aligned to +/- strand): ref base A (5/3); new base C (8/1); total (13/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.20e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCAGGGCCGGCCCTT‑TCGCGGGTGAACCCG‑CTCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTCAACCGCCCCAA > KT2440/3255774‑3256022 | ggCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCCGGGCCGGCCCt > 8:192152/1‑135 (MQ=255) gCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCAGGGCCGGCCCtt < 8:282839/135‑1 (MQ=255) cGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCCGGGCCGGCCCtt‑t > 3:259999/1‑135 (MQ=255) cGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCAGGGCCGGGCCGT‑TCGCgggggcac > 7:147561/1‑130 (MQ=255) cGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGCACGGCGGGGGGAAAT‑TGGCCGGCCGGGCCGGCCCGT‑TCGCGGGGGGACCCG‑cg > 6:296671/1‑134 (MQ=21) gCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCAGGGCCGGCCCTT‑TAGCGGGTGAACCCG‑Cta > 4:104088/1‑133 (MQ=255) ggCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCCGGGCCGGCCCAT‑TCGCGCGTGCACCCC‑CTCCAACGACCGCTTGTGcc > 6:153302/1‑135 (MQ=18) cTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCAGGGCCGGCCCTT‑TCGCGGGTGCACCCG‑CTCCTACGACGGCTTGTGCCGAaccca > 6:100139/1‑133 (MQ=255) aGGAGTGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCAGGGCCGGCCCAT‑TCGCGGGGGGACCCG‑CGCCTACGACGGCTTGGGCCGCACCACCGGAGgacgcc > 3:229854/1‑131 (MQ=14) aGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCAGGGCCGGCCCTT‑TGGCGGGTGGCCCCG‑CGCCTACGACGGCCTGTGCCGCCCCACCGATGgccgac > 7:286361/1‑133 (MQ=14) ggAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCCACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCCGGGCCGGCCCGT‑TAGCGGGGGGACCCG‑CTCCTACGCCGGCGTGTGGCGGGCCACCGCTGGGCGGCGtg > 3:283281/1‑134 (MQ=14) ttACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCCGGGCCGGCCCTT‑TAGCGGGGGAACCCG‑CTCCTACGACCGCTTGTGCCGAACCACCGAGGGGCGACGTAGGCACAggc > 7:239981/1‑133 (MQ=17) aTACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCCGGGCCGGCCCAT‑TCGCGGGTGCACCCG‑CTCCTACGCCGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGCACTGc > 2:221387/1‑135 (MQ=21) tGAACGGCGGGGGGAAAT‑TGGCCGGCCGGGCCGGCCCGT‑TTGCGGGGGGACCCC‑CGCCGACCCCCGCGTGTGGCGCACCCCCGCGGGGCGCCCTAGGGGCCGCCCCCGCACAGCCGCCACCACCGCTcccccccg > 6:239243/1‑129 (MQ=255) ggggggggAAAT‑TGGCCGGCAGGGCGGGCCCTT‑TCGCGGGTGAACCCG‑CTCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTc < 5:239243/133‑1 (MQ=255) gggggggTATT‑TGGCCGGCGCGGCGGGCCCTTGTGGCGGGTCACCCCG‑CCCCAACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTc < 6:16459/134‑1 (MQ=255) ggggggTAATCTGGCCGGCCGGGGCGGGCCTT‑TGGGGGGGCCACCCGCCCCCAACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTc < 3:8552/135‑1 (MQ=255) tgggCCGGCAGGCCCGGCCCTT‑GCGGGGGTCACCCGG‑CTCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTCAACCGCCCCaa < 8:292932/133‑1 (MQ=255) | GGCGAGATTGTAGCGGGTCGGCCGGTGAAGGTGTAGGGCGCAACTCAACCCTTTGTAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGAACGGCGGGGGGAAAT‑TGGCCGGCAGGGCCGGCCCTT‑TCGCGGGTGAACCCG‑CTCCTACGACGGCTTGTGCCGAACCACCGATGGACGACGTAGGCTCAGACCCGGAACTGCCGCAACAACTGCTCCAGCTCTTCGCTCAACCGCCCCAA > KT2440/3255774‑3256022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |