| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,542,102 | 0 | T | G | 48.1% | 8.5 / 10.8 | 27 | intergenic (+31/‑214) | CJ019_01454/groS | hypothetical protein/10 kDa chaperonin |
| Reads supporting (aligned to +/- strand): ref base T (4/10); new base G (13/0); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.53e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATGCGCCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTCACAGCGGGCCA > KT2440/1541966‑1542236 | cATGCGCCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGt > 4:151133/1‑137 (MQ=255) gcCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGtttttt > 8:475755/1‑137 (MQ=255) gcCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGgttttt > 4:166029/1‑137 (MQ=255) gTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGGTTTTTTTTggg > 5:372394/1‑136 (MQ=255) gTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCCCCACC‑GG‑CCCCGGGGTTTTTTTTgg > 1:339715/1‑137 (MQ=255) gTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGAGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑CG‑CCCC‑GGGTTTTTTTTGGc > 4:170250/1‑137 (MQ=255) gTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGCCCACCGCCCG‑AACGTGATCGCAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCCCAACC‑GG‑CCCC‑GGTGATTATTTgcg > 4:289807/1‑135 (MQ=255) tGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGGAAGC‑GG‑CCCC‑GGGTTTTTTTTGGcg > 7:296008/1‑136 (MQ=255) tCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCACGCGGG‑CCCG‑GGTTTTTTTTTggggggtg > 3:205187/1‑131 (MQ=255) ccTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGGCGGGCAAGC‑GG‑CCCC‑CGGTTTTTTTTGGGCGGTTGTAAAAttttatt > 3:281074/1‑134 (MQ=255) cTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCCCCACC‑CG‑CCCC‑GGGGTTTTTTTTGGCTGCTTAAAAAatttagta > 4:329812/1‑133 (MQ=255) cggCGCAGCCGGGTGATGGCGGCCACCGCCCT‑AACGTGAGCGACGGCGAGTCCGCGCCCCGCGAAAAGAACGACGGCCCGGGGCCGCGCAAGC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGa < 8:402577/136‑1 (MQ=255) cAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCtt > 5:500918/1‑137 (MQ=255) cgcgTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGGTTTTTTTTGGCCTGATGAAAAATTTTGTATCTCGAGCCGTGTaa > 4:158992/1‑137 (MQ=255) gcgTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGGTTTTTTTTGGCCTGTTGAAAAATTTTGTATCTGGCGGCTTGTTAt > 5:390706/1‑137 (MQ=255) aTGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGGTTTTTTTTGGCCGGATGTAAAATTTTCTATCTCGCGCCTTGTTATCGGAt > 7:387332/1‑137 (MQ=255) cggcCCCCCCCCG‑AACGGGACGCCAGGCGAGACCGAGCGCCGCTACAAGAAAGACGCCCCGGGCCCGCGCAAGC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTgg < 8:155502/137‑1 (MQ=255) cACCCCCCGCAACGTTACCTACGGCTAGTCCGCGCGCCGCGACACGAACGACGCCCCGGGGCCGCGCAAGC‑GC‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTggcgg < 6:95939/137‑1 (MQ=255) tacggCGAGTACGAGCCCCGCGACAATTAAGAAGGACCGGGGCCGCGCACGC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCAcc < 6:372394/134‑1 (MQ=255) gAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACAGGGGCCGCGCAAGC‑GG‑CCCC‑GGGTTTTTTTTGGCCTGATGAAAATTTTTCTATAGCGAGCCTTGTAATCGGATGTGGCGGGCTCAGGTATGTGGTGAcc > 8:387847/1‑137 (MQ=255) cGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGGTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGCGCCGTGTAAACGGAAGTGGCGGCCTCATGTATGTGGTCACCGCAAg > 8:366224/1‑137 (MQ=255) aCGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGGTTTTTTTTGGGCTGATGAAAAATTTTGTATCTGGAGCCGTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGtttt > 4:474266/1‑136 (MQ=255) cgcgcCGCGACAAGTAAGACGGCCCGGGGCCGCGCAACC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCtgg < 6:390706/136‑1 (MQ=255) aGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCtgg < 6:500918/137‑1 (MQ=255) cccccgcCACAAGTAACACCGCCCGGGGCCGCCCAAGC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCtggt < 5:213505/134‑1 (MQ=255) ccGGGGCCCCCCCAGG‑GGCCCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTAcaca < 3:329812/137‑1 (MQ=255) ggCCGCGCAAGC‑GG‑CACC‑GGGTTTTTTTTGGCCTGATGTAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGGGGTCACCGCAAGGTTTCTGGTGGCCCCACCAGACAGTACACAggtgg > 2:500706/1‑137 (MQ=255) cacgC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTc < 7:264184/134‑1 (MQ=255) cggggccccc‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTAGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTca < 1:417006/131‑1 (MQ=255) ggTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTCACAGCGGGCCa > 3:439720/1‑137 (MQ=255) | CATGCGCCAGCGTGCATTCCAGGATGACCCCTTCCAGGCGCAGCCGCGTGATGGCGGCCACCGCCCG‑AACGTGATCGAAGGCGAGTACGAGCGCCGCGACAAGTAAGAAGGACCGGGGCCGCGCAAGC‑GG‑CCCC‑GGTTTTTTTTTGGCCTGATGAAAAATTTTCTATCTCGAGCCTTGTAATCGGATGTGGCGGCCTCATGTATGTGGTCACCGCAAGGTTTCTGGTGGCAACACCAGACATTACACAGGTGGTTCGCTCACAGCGGGCCA > KT2440/1541966‑1542236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |