Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,752,3690GC34.3% 33.2 / 10.9 36A523G (GCC→GGC) CJ019_02595hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (11/12);  new base C (0/12);  total (11/25)
Fisher's exact test for biased strand distribution p-value = 5.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGGGTCAATCCTATGACTGAAACGCTGCAGTTTTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATGGGCTCGCCGTGAGTCTCGCCATCCCAGTGCGTCACCCCCAGCGAAATCGTCACGG  >  KT2440/2752240‑2752498
                                                                                                                                 |                                                                                                                                 
ggggTCAATCCTATGACTTAAACGCTGCAGTTTTATGACAGGAATCGTAACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGc                                                                                                                            <  8:144621/137‑1 (MQ=255)
 gggTCAATCCTATGACTGAACCGCTGCAGTTTTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGAATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGAAAAAGCg                                                                                                                           <  3:186742/137‑1 (MQ=255)
         ccTATGACTGAAACTCGGCAGTTTTATGACAGGAATTGTTACAAGCCACCTAATCATCGTTGCCTTTACCGGAATTTTCACGGGCAACCCCGCCCGTTGGGTACAACGCACTACGTTCAGGAAAAAGCGACCCGGtt                                                                                                                   <  7:449051/137‑1 (MQ=255)
         ccTATGACTGAAACGCTGCAGTTTTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGtt                                                                                                                   >  8:190228/1‑137 (MQ=255)
         ccTATGACTGAAACGCTGCAGAATTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGCCATAATCACGGGAAAGCCCGCCCGATGCGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGtt                                                                                                                   >  5:153913/1‑137 (MQ=255)
          cTATGACTGAAACGCTGCAGTTTTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTg                                                                                                                  >  2:73856/1‑137 (MQ=255)
           tATGACTGAAACGCTGCAGTTTTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTgc                                                                                                                 >  4:91653/1‑137 (MQ=255)
              gACGGAAACGCTGCAGTTTTATGACAGCACTTGTAACAACCCACCGAATCATCGTTGCCTTTACCGGCATTTACACGGGCAAGCCCGCCCTATGGGTACAACCCAACCCGTTCAGCCAAAAGCGACCCGGTTGCGcc                                                                                                              <  8:435578/137‑1 (MQ=255)
                cTGAAACGCTGCAGTTTTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGcccc                                                                                                            >  5:254051/1‑137 (MQ=255)
                cTGAAACGCTGCAGTTTTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGcccc                                                                                                            >  2:162269/1‑137 (MQ=255)
                    aaCGCTGCAGTTTTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGAAAGACGTTCAGCCAAAAGCGACCCGGTTGCGCCCCTctt                                                                                                        <  1:76169/137‑1 (MQ=255)
                         tGCAGTTTTATGACAGTAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGTTGGGTACAACGCAAGACGTGCAGCCAAACGCGACCCGGTTGCGCCCCTcttgcttg                                                                                                   <  7:382116/137‑1 (MQ=255)
                                   tGACAGAAATTGTAACAACCCCCCCAATCATCGTTGCCTTAACCGGCTTTATCCCGGCCACCCCCGCCCTATGGTAACACCGCAACCCGTTCAGCAAAACGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTaaa                                                                                         <  2:218537/137‑1 (MQ=255)
                                         taaTTGTTACAAGCCACCGAAGCATCGTGGCCTTTACCGCCATTTTCACGGGCACGCCCGCCCGATGGGTACAACGCAACACGTTCAGCCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGccc                                                                                   <  7:58167/136‑1 (MQ=255)
                                         gAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGccc                                                                                   <  6:254051/137‑1 (MQ=255)
                                         caaTTGTTACAAGCCCCCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGccc                                                                                   <  7:79052/136‑1 (MQ=255)
                                         aaaTTGTTACAACCCACCGAATCATGGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGTTACAACGCAAGACGTTCAGTAAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGccc                                                                                   <  8:459510/136‑1 (MQ=255)
                                           aTTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCgg                                                                                 >  1:326160/1‑137 (MQ=255)
                                             tGTTACAACCCACCCAATCTACGTTGCATTATCCGGATATTCCCCGCTCAAGCCCGCCCGAGGGGTAAACCGCAAGACTTACAGGCAAACGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCTGTAAAGCGCCCGGTc                                                                               <  2:465592/137‑1 (MQ=255)
                                             tGTAACACGCCACCGAAACATCGTGGCCTTACCCGCCATTTTCCCGGGCAAGCCCGCCGGATGGGTACAACGCAAGACGTGCAGGCAAACGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTc                                                                               <  8:319199/137‑1 (MQ=255)
                                              gtaacacGCCCCCCAATCATCGTTGCCTTAACCGTAATTTTCCCGGCCACGCCCGCCCGTTGGGGACAACCCAAGCCGTGCAGCCAAACGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCa                                                                              <  1:311640/134‑1 (MQ=255)
                                                   accccACCTAATCATCGTTCCCTTTCCCGGCATTTTCACGCGCAAGCCCGCCCGATGGGAACAACGCACGACGTACAGCCAAACGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTc                                                                         <  2:116219/134‑1 (MQ=255)
                                                   aacccACCGAATCATCGTGGCCTTTACCGGCATTTTCACGGGCACGCCCGCCCGATGGGTACACCGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTc                                                                         <  8:348426/134‑1 (MQ=255)
                                                    aGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCg                                                                        >  8:322559/1‑137 (MQ=255)
                                                            aaTCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTgc                                                                >  6:129735/1‑137 (MQ=255)
                                                              tcatcGTTGCCTTTACCGGCATTTTCACGGGCACGCCCGCCCGATGGGTACAACGCACGACGTTCAGCAAAACGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTgcgc                                                              <  3:91653/137‑1 (MQ=255)
                                                                    tggCCTTAACCGCCTTTTTCACGGCCACGCCCGCCCGATGGGAACACCCCACGCCGTCCCGCCAAACGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATgg                                                        <  3:40445/135‑1 (MQ=255)
                                                                         tttCCCGCCATTTACCCGGGACAGCCGGCCCGATGGGAAAACCGCAAGACGTTCAGCCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATGGGCTCg                                                   <  3:250356/137‑1 (MQ=255)
                                                                                  aTTTTCACGGGCAAGCCCGCCGGATGGGTACAACGCAAGACGTTCTGGCAAAAGCGACCCGGCTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATGGGCTCGCCGTGAGtc                                          <  6:428427/137‑1 (MQ=255)
                                                                                  aTTTTCACGGGCAAGCCCGCCCGATGGGTACACCGAAAGAGGTACAGGCAAAAGCGACCCGGGTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATGGGCTCGCCGTGAGtc                                          <  4:424783/137‑1 (MQ=255)
                                                                                          gggCAAGCCCGCCCGATGGGAACAACGCAATACGTGCCGCCAAACGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATGGGCTCGCCGTGAGTCTCGCCATc                                  <  7:190228/137‑1 (MQ=255)
                                                                                                                 aaCGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTgcgc                                                              >  7:440626/1‑86 (MQ=255)
                                                                                                                 aaCGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTgcgc                                                              <  8:440626/86‑1 (MQ=255)
                                                                                                                   cGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATGGGCTCGCCGTGAGTCTCGCCATCCCAGTGCGTCACCCCCAGCGAAATc         >  6:419682/1‑137 (MQ=255)
                                                                                                                          cGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATGGGCTCGCCGTGAGTCTCGCCATCCCAGTGCGTCACCCCCAGCGAAATCGTCACgg  <  7:322559/137‑1 (MQ=255)
                                                                                                                          cGTTCAGCCAAACGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATGGGCTCGCCGTGAGTCTCGCCATCCCAGTGCGTCACCCCCAGCGAAATCGTCACgg  <  3:152449/137‑1 (MQ=255)
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GGGGTCAATCCTATGACTGAAACGCTGCAGTTTTATGACAGGAATTGTTACAAGCCACCGAATCATCGTTGCCTTTACCGGCATTTTCACGGGCAAGCCCGCCCGATGGGTACAACGCAAGACGTTCAGGCAAAAGCGACCCGGTTGCGCCCCTCTTGCTTGGCCCGGTAAAGCGCCCGGTCAGCCTCGCCCAGTGCGCTCATGGGCTCGCCGTGAGTCTCGCCATCCCAGTGCGTCACCCCCAGCGAAATCGTCACGG  >  KT2440/2752240‑2752498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑