| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 690,144 | A→G | intergenic (+21/‑2438) | bauC → / → PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 690,144 | 0 | A | G | 92.3% | 15.6 / ‑6.5 | 13 | intergenic (+21/‑2438) | bauC/PP_RS08750 | Putative 3‑oxopropanoate dehydrogenase/tRNA‑Val |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (12/0); minor base C (1/0); total (13/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGAGGCCGTCA‑GGCC‑TCCCCGC‑TTTGTTTTTGGGGGTTGGTTGTT‑GCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCG > KT2440/690011‑690237 | aTGCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑ggggggg > 1:84707/1‑136 (MQ=255) ggCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGCCgggaggg > 2:59390/1‑131 (MQ=255) ggTGTGCGCTTCTATACCCGTCGCAAGGACATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGCCCTGA‑GGCC‑Gccccccg > 8:285134/1‑134 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGGCGGCC‑CGCC‑GGCCCCC‑GTTGGTTTTTGGGGGTGggtgtt > 4:317507/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGGCGGCA‑GGCC‑CCCCCGC‑GTTGTTTTTTGGGGGTGgttgtt > 6:318144/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGGCCGCA‑GGGC‑TCCCCGC‑GTTGGTTTTGGGGGGTGgttgtt > 5:508607/1‑137 (MQ=255) gCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGCGGGCGGGA‑GGCCGCCCCCGG‑TTTGTTTTGGGGGGTGggtggt > 6:336362/1‑137 (MQ=255) cAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGCCGGCA‑GGCCTTCCCGGG‑TTTGTTTTTGGgggtgggtgttt > 7:367005/1‑133 (MQ=255) aaGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATCAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCGTGGGGGCCCGGTA‑GCCC‑GCCCC‑C‑GATGTTTTTTGGGGGTGggtgtttg > 8:150520/1‑135 (MQ=255) cccAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGGCGGCC‑GGCC‑GCCCCGCGGTTGTTTTTGGGGGTTGG‑TGTT‑GGTGGTgggggg > 5:384409/1‑135 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGGCGTGG‑GGCC‑ACCCCGC‑GTGGTTTTTTGGGGGGGGTTGTTGGGTGTTGGGGGGGaagg > 6:92226/1‑135 (MQ=255) gTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGGCGGGA‑GGGC‑GCCCCCC‑GGTTTTTTTTGGGGGTGGGTGTT‑GGTGTTGggggggggcgg > 5:254012/1‑132 (MQ=255) tGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGGCGGGACGGCC‑CCCCCCC‑GTGTTTTTTGGGGGTGGGTGTTT‑GGGGTGGGgggggacggtt > 6:162683/1‑132 (MQ=255) aGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGGGGGCGGGAGGGCC‑GCCCCCG‑GTGTTTTTTGGGGGTGGGTTG‑T‑GGGGGTGGGGGGGGTCTGTTTTTTTTGGGGGTTGGGTGTGTGGTGTGTGCg > 8:455828/1‑137 (MQ=255) | ATGCCTATGGCCCGGATGGTGTGCGCTTCTATACCCGTCGCAAGGCCATTACCCAGCGTTGGCCGCAGCGGGCCAGCCATGAAGCGTCGCAGTTTGCATTCCCTAGCCTGTAAGGGAAGTAGATGAAGCG‑GGGAGGCCGTCA‑GGCC‑TCCCCGC‑TTTGTTTTTGGGGGTTGGTTGTT‑GCTGTTGGGGAGGAACTGTTTTGAGAGGGTGTTGGCTGTGAGATGTGTCCG > KT2440/690011‑690237 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |