Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,880,221	A→C	27.3%	V96G (GTG→GGG)	CJ019_04574 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,880,221	0	A	C	27.3%	70.4 / 4.2	53	V96G (GTG→GGG)	CJ019_04574	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (12/25);  new base C (14/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 2.74e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCACTTCAGGCCG  >  KT2440/4880091‑4880354                                                                                                                                   |                                                                                                                                       ccGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGcacc                                                                                                                                      <  8:74662/133‑1 (MQ=255) ccGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTAGCGCCGCTGATCCCCACGGCACCCCGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGcacc                                                                                                                                      <  3:276222/133‑1 (MQ=255)  cGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGCTCCCCACGGCCCCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCcccacc                                                                                                                                   >  8:250198/1‑135 (MQ=255)    cAGCCCCACCTCCTCGATCGCACTGATCTCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCTGCGCCGGCACCACCgg                                                                                                                                 <  8:62448/135‑1 (MQ=255)    cAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCgg                                                                                                                                 <  3:150731/135‑1 (MQ=255)    cAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCgg                                                                                                                                 <  5:221571/135‑1 (MQ=255)    cAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCgg                                                                                                                                 <  7:71122/135‑1 (MQ=255)    agacagcACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCgg                                                                                                                                 <  7:382876/129‑1 (MQ=255)      gCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGcc                                                                                                                               <  2:273466/135‑1 (MQ=255)       ccccACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGCTCACGAATCAGCACCCCACCCGGCGCCGGCCCCCCCGGCCg                                                                                                                              >  7:128466/1‑135 (MQ=255)       ccccACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCCGCCCCCGCCCCCCCCGccc                                                                                                                              >  1:4602/1‑134 (MQ=255)       ccccACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCg                                                                                                                              >  8:231545/1‑135 (MQ=255)       ccccACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGAGCACGAATCAGCACCCCACCCGGCCCCCGCCCCCCCGGCCg                                                                                                                              >  4:234124/1‑135 (MQ=255)         ccACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑t                                                                                                                           <  1:366322/135‑1 (MQ=255)               cctcGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCCGCCCCCCCGGCCGC‑CCCCCCa                                                                                                                     >  2:144916/1‑135 (MQ=255)                    aTCGCACTGATCGCACACTGCTCGTCGATATCAGACGTATCGCCGCTTATCCCCACGGCACCCAGCAACTTGCAGTCCTCAACACGAATCAGCACACCACCCGCCGACCGCCAAACACGCCGC‑TCACCCAAcccc                                                                                                                >  4:380027/1‑134 (MQ=255)                        cACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCCGCGCCCGCCCCCCCCGCCGC‑TCACCCAAcccagtct                                                                                                            >  8:382876/1‑130 (MQ=255)                           tGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAAcg                                                                                                         >  4:42630/1‑135 (MQ=255)                                gCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCCGCCCCCCCGGCCGC‑GCCCCCATACCCGTCAAccccgc                                                                                                     >  3:17103/1‑129 (MQ=255)                                      tGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCCGCACAACCCGCCGC‑TCCCCCAACCACTTCAACCCCGCaaaaaaa                                                                                              >  2:350783/1‑135 (MQ=255)                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCCCCCAGCACCTTGCCGTCCTGATAACGAATCAGCACCCCCCCCGCCGCCGGCCACAACGGCCGC‑AGACCCCTCCCGGTCAACGCGGCAAAAAAAcccg                                                                                          >  7:98138/1‑135 (MQ=255)                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCACCGGCCGC‑TCACCCATCCCCGTCCACGCGGCCACAAAAgccc                                                                                          >  7:277320/1‑134 (MQ=255)                                          cgtcgATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGCGGCCCCCATCCCGGTAAACCCGGCAAAAAAAccc                                                                                           >  1:320762/1‑135 (MQ=255)                                               atatCCGACGTATCGCCGCTGATCCCAACGGCACCCAGCACCTTGCCGTCCTGATCACCAATCAGCACCCCACCCGGCGCCGGCACCAACCGCCGC‑GCCCCCCACACGGTGAACGCGGCGAAAAAAaccggcccc                                                                                     >  4:320527/1‑133 (MQ=255)                                                   cctccGTTTCGCCGCTGACCACCACGGCACCCAGCACCTTGCCGGCCTGATCACCAATCACCACCCCACCCGGCGCCGGCACCCCCGGCCGC‑GCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCgctgct                                                                                 <  6:149822/131‑1 (MQ=255)                                                   ccGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCCCCCCCCCCGGCGCCGGCCCCCCCGGCCGC‑TCCCCCAAACCCTTCAACGCGGCAAAAAAAgcccgccccagca                                                                                 >  4:35686/1‑129 (MQ=255)                                                   ccGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCCGCGCCCGCACCACCCGCCGC‑GCCCCCAACCCCTTCAACCCCGCAAAAAAAgcccgccccggca                                                                                 >  2:312533/1‑129 (MQ=255)                                                     gACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCCCCCGGCGCCGGCCCCCCCCGCCGC‑GCCCCCATCCCGGTCAACCCGGCAAAAAAAGCCGGCCGCggcggc                                                                               >  7:228264/1‑135 (MQ=255)                                                                  tGATCCCCCCGGCACCCACCACCTTGCCGTCCTGATCACCAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCa                                                                  <  2:4602/135‑1 (MQ=255)                                                                        ccACGGCACCCCGCACCTTGCCGTCCTGAGCACGAATCCGCACCCCACCCGGCGCCGGCACCCCGGGCCGC‑TCACCCATCCCGTTCCCCGCCGCAAAAAACGCCGGCCGCTGCTGCGCATCCAGCGCCAGCATgc                                                            <  4:291832/135‑1 (MQ=255)                                                                            ggCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCCCCCCCGGCCGC‑TCACCCCTCCCGGTAAACGCGGCAAAAAAAGCCGGCCGCAGCGGCGCCTACACCGCCAGCAAccgcca                                                        >  4:285047/1‑133 (MQ=255)                                                                             gCACCCCGCACCTTGCCGCCCTGATCACGACTCCGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAACGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAg                                                       <  1:16273/135‑1 (MQ=255)                                                                             gCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACGGGCCGC‑TCACCCAACCCGTGCACCGCGCAAAAAAACGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAg                                                       <  1:312533/135‑1 (MQ=255)                                                                             gCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAg                                                       <  8:356305/135‑1 (MQ=255)                                                                             ccacccaccCCCTTGCCGTCCTGACCACGAATCAGCACCCCACCCGCCGCCGGCCCCCCCGGCCGC‑TCCCCCATCCGGTGCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAg                                                       <  1:261733/134‑1 (MQ=255)                                                                                  catcACCTTGCCGTCCTGATCACGAATCAGCCCCCCACCCGGCGCCGGCACCACCGGCCGC‑GCACCCATCCCGTTCAACGCGGCAAAAAATGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCtt                                                  <  4:320582/132‑1 (MQ=255)                                                                                  cAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCtt                                                  <  7:231545/135‑1 (MQ=255)                                                                                  cAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGCCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCtt                                                  <  1:65162/135‑1 (MQ=255)                                                                                   aGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCCCCGGCCGC‑TCCCCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCCACCCGCGCCAGCAAGCGCGCGCCCTTg                                                 >  4:159166/1‑135 (MQ=255)                                                                                   aGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGGTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCCTCCAGCGCCAGCCAGCGCCCGCCCGTg                                                 >  3:305413/1‑135 (MQ=255)                                                                                   aGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCCCCGGCCCCCCCGGCCGC‑TCCCCCCAACCCTTCAAAGCGGCAAAAAAAACCCGCCCCGGCAGCGCCTACACCGCCCGCCACCCCGAGCCCATg                                                 >  1:278788/1‑135 (MQ=255)                                                                                      aCCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCCCCACCGGCCGC‑GCCCCCCTCCCGTTCCACGCGGGAAAAAAAGCCGGCCGCTGGGGGGCCTCCCGCGCCCGCCAGCGCGCCCCCGTGccc                                              >  2:43015/1‑135 (MQ=255)                                                                                            ccGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGc                                         <  8:228264/134‑1 (MQ=255)                                                                                             cGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑GCCCCCAACCCGTTCAACCCGGCAAAAAAACCCCGCCGCGGCGGCGCAACCCGCGCCACCCACCCCGCGCCCATGCCCAggggc                                        >  2:296840/1‑132 (MQ=255)                                                                                             cGTCCTGAGCACTAATCAGCACCCCCCCCGGCGCCGGCACCACCGGCCGC‑TCACCCCCCCCGTGCACCGCGCCAAAAAACGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCaa                                       <  5:126250/135‑1 (MQ=255)                                                                                                  tGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCCTCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCCGCGCCCGCAAGCCCCCGCCCATTCCCACGGCCATCGCa                                  >  2:209375/1‑135 (MQ=255)                                                                                                  tGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCCACGCCGCAAAAAAAGCCGGCCCCTGCTGCGCCTCCAGCGCCCGCAAGCCCGCGCCCATGCCCACGGCAATCGcc                                  >  2:215943/1‑134 (MQ=255)                                                                                                    aTCACGAATCAGCACCCCACCCCGCCCCCGCACCACCCGCCGC‑TCACCCATCCCCTTCAACCCCGCAAAAAAAGCCCGCCGCTACTTCGCATCCAGCGCCACCAACCGCCCGCCCTATCCCACGGCCATCGcccc                                >  4:162535/1‑135 (MQ=255)                                                                                                         gAATCAGCACCCCCCCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAgg                           <  1:209375/135‑1 (MQ=255)                                                                                                         gAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCACCGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAgg                           <  1:215943/135‑1 (MQ=255)                                                                                                               gCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTg                      <  8:332480/134‑1 (MQ=255)                                                                                                                  ccccacccGGCGCCGGCACCACCGGCCGC‑GCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAg                  <  4:17103/135‑1 (MQ=255)                                                                                                                   cccccccGGCGCCGGCCCCACCGGCCGC‑TCCCCCAACCCGTTCACCGCGCCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGt                 <  1:362798/135‑1 (MQ=255)                                                                                                                   cccacccGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGGTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGt                 <  2:259129/135‑1 (MQ=255)                                                                                                                        ccGGCGCCGCCACCCCCGGCCGC‑GCCCCCATCCGGGTCCCCGCGCCAAAAAACCCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCa            <  7:144595/135‑1 (MQ=255)                                                                                                                            cgcCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCACTTc        <  6:139395/135‑1 (MQ=255)                                                                                                                                  caccccCGGCGGC‑TCCCCCTACCGGTCCAACGCGGCAAAAAATGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCACTTCAGGCCg  <  3:35686/135‑1 (MQ=255)                                                                                                                                  caccccCGGCCGC‑GCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCACTTCAGGCCg  <  4:75956/135‑1 (MQ=255)                                                                                                                                  caccccCGCCCGC‑TCCCCCACCCCATACAACCCCCAAAAAAACGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCACTTCAGGCCg  <  1:185858/135‑1 (MQ=255)                                                                                                                                  caccacGGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCACTTCAGGCCg  <  2:320762/135‑1 (MQ=255)                                                                                                                                  caccacCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAACGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCACTTCAGGCCg  <  3:285047/135‑1 (MQ=255)                                                                                                                                  caccacCGGCCGC‑GCACCCCTCCCGTGCAACGCGGCAAAAACAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCACTTCAGGCCg  <  2:233085/135‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       CCGCAGCCCCACCTCCTCGATCGCACTGATCGCACACTGCTCGTCGATATCCGACGTATCGCCGCTGATCCCCACGGCACCCAGCACCTTGCCGTCCTGATCACGAATCAGCACCCCACCCGGCGCCGGCACCACCGGCCGC‑TCACCCATCCCGTTCAACGCGGCAAAAAAAGCCGGCCGCTGCTGCGCATCCAGCGCCAGCAAGCGCGAGCCCTTGCCCAGGGCAATCGCACCCCAGGCTTTGCCAGTGGCCACTTCAGGCCG  >  KT2440/4880091‑4880354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑