Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	399,292	A→C	35.1%	intergenic (‑186/+122)	CJ019_00365 ← / ← aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	399,292	0	A	C	35.1%	32.0 / 4.3	55	intergenic (‑186/+122)	CJ019_00365/aceF	hypothetical protein/Dihydrolipoyllysine‑residue acetyltransferasecomponent of pyruvate dehydrogenase complex
Reads supporting (aligned to +/- strand):  ref base A (17/15);  new base C (8/9);  total (30/25)
Fisher's exact test for biased strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAAGGTTGCATGATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCAG  >  KT2440/399157‑399422                                                                                                                                           |                                                                                                                                    gCAAGGTTGCATGATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGAC‑GGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAagga                                                                                                                                     >  7:65288/1‑135 (MQ=255)         gCATGATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGaa                                                                                                                              >  4:105882/1‑135 (MQ=255)             gatgTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGccc                                                                                                                          <  7:33361/135‑1 (MQ=255)                  cccGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GGAAGGAGGGCGCATCCCGCCCa                                                                                                                     >  3:203153/1‑135 (MQ=255)                  cccGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GGAAGGAGGGCGCAGcccaccca                                                                                                                     >  5:381439/1‑135 (MQ=255)                    cGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GTGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCAGc                                                                                                                   >  5:388880/1‑135 (MQ=255)                       gggCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGCAGCCCTCCCAGCatt                                                                                                                >  8:225889/1‑135 (MQ=255)                         gCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGCGGGCGCAGCCCTCCCCGCgtttt                                                                                                              >  8:181326/1‑135 (MQ=255)                         gCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCAGCgtttt                                                                                                              >  2:204818/1‑135 (MQ=255)                              cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTGGC‑GGACGGGCGGCGCAGCCCGCCCCGCgttttggagg                                                                                                         >  5:392625/1‑130 (MQ=255)                              cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGCGGGCCAAGCCCTCCCAGCATTTTCCAg                                                                                                          >  3:301822/1‑134 (MQ=255)                              cAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTGCCAGCtgtttggag                                                                                                          >  7:123353/1‑130 (MQ=255)                                aGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGCAGCCCTCCCAGCGTTTTGCAGGc                                                                                                        >  7:125298/1‑134 (MQ=255)                                     ccATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGCAGCCCTCCCAGCGTTTTCCAGGC‑aaaaa                                                                                                  >  8:172909/1‑134 (MQ=255)                                               tGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGCGGGCGAATCCCTCCCAGCTTTTTGCAGGC‑AAAAAAAAGCCCCGc                                                                                        >  3:93782/1‑134 (MQ=255)                                                 cTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGCGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCaa                                                                                     >  4:76738/1‑135 (MQ=255)                                                    gggACTGGTTATGGGCGGCCGTGGCCTTTTCGGCTGTTTGGCGGGTTTTTTG‑GGGGCCGGTTT‑GGGGCGTGGTGCCGC‑CAAAGGAGGGCGAAGCCCGCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCg                                                                                  <  8:14168/134‑1 (MQ=255)                                                     ggccAGGTTCTCGGCTGCCGTGGCCGATTCTGCCGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTGGTGTCGC‑GAAAGGCGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCg                                                                                  <  3:311619/131‑1 (MQ=255)                                                      gACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGGAGCCCTCCCAGCATTTTCCAGGC‑AAAAAAAACCCCCGCCAATCgcc                                                                                >  4:352344/1‑133 (MQ=255)                                                       aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACg                                                                               >  4:334091/1‑135 (MQ=255)                                                       aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACg                                                                               >  7:200430/1‑135 (MQ=255)                                                       aCAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCCCGGAAAGGGGGCCACACCCCGCCCCGAGTTTT‑CAGGC‑AAAAAAAAACCCCCCCCATAGAcc                                                                               >  7:346618/1‑134 (MQ=255)                                                        cAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGGCGGGTTT‑GCGGCGTGGTTTGGC‑GAAAGGGGGGCGAGCCCCTCCCCCTTTTTTCAGGGC‑AAAAAAAGGCCCCGCCAAtgcccg                                                                               >  2:315254/1‑130 (MQ=255)                                                        cAGGTTATCGGCGGCCGTGGCCGATTCTGCAGTTGGGCGGGAGATGTG‑GGGGCCGCTTT‑GCGGCCTTGTGGCGC‑GAAAGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACgg                                                                              <  7:207102/135‑1 (MQ=255)                                                            ttATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGGCTTGTGTGGC‑GAAAGGGGGGGGCAGCCCGCCCCGCTGTTTGGCGGCAAAAAAAAAGCCCCGCCAAACGACggggg                                                                           >  1:374193/1‑134 (MQ=255)                                                            ttATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCGTT‑GCGGCCGTGTGTGGC‑GCACCGAGGGCGGAGCCCGCCCAGCGTTGTTCAGGC‑AAAAAAAACCCCCCCCCATCCCCGGGGtt                                                                          >  6:228418/1‑135 (MQ=255)                                                              aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTTGGGGGCCGGTTT‑GCGGCGTTGGGTGGG‑GAACGGGGGGCGAGCCCGGCCCCGGTTTTGCCAGGC‑AAAAAAAGCCCCGGCCAAACGAGGGGGTTg                                                                         >  8:271626/1‑135 (MQ=255)                                                              aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GGAAGGCGGGCGCAGCCCGCCCCCCGTTTTGCAGGC‑CAAAAAAACCCCCCCCAATCGGCCGGGGTGa                                                                        >  7:163954/1‑135 (MQ=255)                                                              aTCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAACGCCGGCGACGCCCTCCCCCCTTTTTGCACGC‑AAAAAAAAGCCCCGCCCAACGACCGGGTTgt                                                                        >  1:248409/1‑134 (MQ=255)                                                                 ggCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GCAATGCGGGCGAAGCCCACCCCGCGTTTTCCAGGC‑AAAAAAAACCCCCGCCCATAGACGGGGTTTAGGt                                                                     >  8:161237/1‑135 (MQ=255)                                                                 ggCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGGGAAGCCCTCCCAGCTTTTTGCAGGC‑AAAAAAAAACCACGCCAATCGCCGGGGTTGAGGt                                                                     >  8:156566/1‑135 (MQ=255)                                                                   cTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GGAAGGCGGGCGAAGCCCTCCCAGCGTTTTCGAGGG‑AAAAAAAAGCCCCGCCAATCGCCGGGGGTGTGGTAc                                                                   >  7:139808/1‑135 (MQ=255)                                                                                  cTGCAGTTGGGGTGGAGATTTG‑GGGGCGGCTTT‑GCGGCCGTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTc                                                     <  4:117752/134‑1 (MQ=255)                                                                                    gtaGTTTGGCTGGTGTTTTT‑GGGGCCGCTTT‑GCGGCTTTGTGTCCC‑GAAATGTGGGCTAAGCCCTCCCTGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGa                                                   <  7:225889/132‑1 (MQ=255)                                                                                             cTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCTCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACt                                         >  3:377020/1‑135 (MQ=255)                                                                                             cTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGGGGCGC‑GAAAGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACt                                         <  7:250012/135‑1 (MQ=255)                                                                                                ttgaTTTT‑GGGCCGGCTTT‑GGGGCCTTGTGGCGC‑AAAAGGAGGGCCAAGCCCTCCCAGCTTTTTCCAGGA‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGcc                                      <  6:381439/133‑1 (MQ=255)                                                                                                    tttt‑GGGGGCGGTTTGGGGGCGTTGGGCCGC‑GACCGGCGGCCAAACCCCGCCCAGCTTTTTCCCGGC‑AAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGcccc                                    <  6:322204/134‑1 (MQ=255)                                                                                                     ttt‑GGGGCGGCTTT‑GCGGCCTTGTGTCGC‑TAATGGAGGGCTAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTc                                  <  4:93782/134‑1 (MQ=255)                                                                                                     ttt‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCACCTTTTTCCAGGC‑AAAAAAAAGCCCCCCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTTCCCCTc                                  >  5:151424/1‑134 (MQ=255)                                                                                                     ttt‑GGGGCCGCTTT‑GCGGCCGTGTGGCGC‑GACCGGCGGGCGAAGCCCGCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTc                                  <  3:105882/134‑1 (MQ=255)                                                                                                     ttggggggCCACTTG‑GCGGCCGGGTGCCGC‑GAAAGGCGGGCGAAGCCCGCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTc                                  <  7:377039/131‑1 (MQ=255)                                                                                                     gttgggggCGGCTTG‑GGGGCCGGGGGCCGC‑CAACGGCGGGCGAAGCCCCCCCAGCTTTTTCCCGCC‑AAAAAAACGCCCCGCCACTCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTc                                  <  6:308254/131‑1 (MQ=255)                                                                                                       t‑GGGGCGGTTTG‑GGGGCGTTGGGGCGC‑CAAGGGCGGGCCACCCCCCCCCAGCTTTTTCCAGCA‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGc                                <  5:7922/134‑1 (MQ=255)                                                                                                        gggggCCGCTTG‑GGGGCCTGGTGGCGC‑GAACGGCGGGCGCCCCCCGCCCAGCTTTTTCCAGCC‑AAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGGGGCAGCTCGAAAAAGGTACTGCCCCTCGc                                <  7:27178/133‑1 (MQ=255)                                                                                                               gCTTT‑GCGGCCGTGGGTCGC‑GACAGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAggg                        <  2:274509/135‑1 (MQ=255)                                                                                                                      gggCCTGGTGGCGC‑CAACGGCGGCCGAACCCCACCCAGCTTTTTCCACGC‑AAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgtg                  <  1:224799/134‑1 (MQ=255)                                                                                                                      cGGCGTTGTGTCCC‑GACCGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCACTCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                   <  4:48581/134‑1 (MQ=255)                                                                                                                      cGGCCTTGTGGCGC‑GAAGCTCGCGCCAACCCCCCCCAGCTTTTTCCAGCC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                   <  6:391453/134‑1 (MQ=255)                                                                                                                      cGGCCTTGGGGCCC‑GAACGGAGGGCGAAGCCCTCTCAGCTTTTACCAGGC‑AAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgtg                  <  4:377020/135‑1 (MQ=255)                                                                                                                      cGGCCTGGTGTCGC‑GAACGGAGGGCGACGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAACGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                   <  8:65288/134‑1 (MQ=255)                                                                                                                      cGGCCTGGTGTCGC‑CAACGGAGGGCGAAGCCCTCCCAGCGTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgt                   <  2:75232/134‑1 (MQ=255)                                                                                                                      cGGCCTGGGGACCC‑GAAAGGAGGGCGAAGCCGGCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAgtg                  <  3:180671/135‑1 (MQ=255)                                                                                                                                 cgc‑gAAAGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGCC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTa        <  8:139808/134‑1 (MQ=255)                                                                                                                                      aaaGGAGGGCGACGCCCTCCCAGCTTTTTCCAGCC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGGACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagca   <  8:284209/135‑1 (MQ=255)                                                                                                                                       aaGGAGGGCGAAGCCCTCCCAGCTTTTTCCCGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTAcagcag  >  6:170172/1‑135 (MQ=255)                                                                                                                                           |                                                                                                                                    GCAAGGTTGCATGATGTCCCGAGGGCTGGCAAGCGGCCATGCTTGATGCTGTGGACAGGTTATCGGCTGACGTGGCCGATTCTGCAGTTTGGCTGGAGATTTT‑GGGGCCGCTTT‑GCGGCCTTGTGTCGC‑GAAAGGAGGGCGAAGCCCTCCCAGCTTTTTCCAGGC‑AAAAAAAAGCCCCGCCAATCGACGGGGTTGAGGTACGAGCGTGGCAGCTCGAAAAAGGTACTGCCCCTCGCAAGGAGGGGCAGTGTGCTGGCTTACAGCAG  >  KT2440/399157‑399422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑