| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 759,433 | A→C | 48.4% | intergenic (+42/‑126) | mmuM_1 → / → CJ019_00729 | Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 759,433 | 0 | A | C | 48.4% | 4.0 / 18.0 | 26 | intergenic (+42/‑126) | mmuM_1/CJ019_00729 | Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase |
| Reads supporting (aligned to +/- strand): ref base A (3/9); new base C (10/3); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01 | |||||||||||
ACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCTTTT‑GCAG‑CGCCCGG‑CCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCATCGGTGTACACAACTCAGCTGGGCGGGA > KT2440/759303‑759551 | aCTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGGGTTT‑GCAG‑CGCCCCG‑CCCGCCGCCa > 7:26901/1‑135 (MQ=255) cTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGGCCCG‑GGCGGGTTTT‑GC‑G‑CGCCCCG‑CCCgccgccc > 3:127549/1‑133 (MQ=255) cGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCGGTT‑GGAG‑CGCCCGC‑CCCCCCGCCATTat > 7:43969/1‑133 (MQ=255) gTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGGGGCTTTT‑TCGG‑CGCCCCG‑GCCGCAGCCGATaaa > 7:84374/1‑134 (MQ=255) gTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCTTTT‑TGAG‑CGCCCGG‑CCCGCCGCCAATaaa > 2:22065/1‑134 (MQ=255) gTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCTTTT‑GCCG‑CGCCCCG‑CCCGCCGCCATTaaa > 4:51800/1‑134 (MQ=255) gTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCGTTT‑GCAG‑CGCCCGG‑CCCGCAGCCATTaaa > 4:260545/1‑134 (MQ=255) gCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCACCCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCGTTT‑GCCG‑CGCCCGG‑GCCGGAGCCATTAAAAAAAACGcccgg > 2:47447/1‑132 (MQ=255) ggcggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGACACGCACCCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGGTGTT‑GCCG‑CGCCCCG‑CCCGCCGCCAGTTTAAAAAACGCCAGGCCGCCCCggcgt > 7:387135/1‑132 (MQ=255) ggcggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCTTTT‑GCAG‑CGCCCGG‑CCCGCCGCCATTAAAAAAAACGCCAGGCAGCCCCGGCtt > 7:95164/1‑135 (MQ=255) ggTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCC‑GGGGGCGTTT‑TCAG‑CGCCCCG‑CCCGCCGCCCGTAAAAAAAAACCCCGTCGGACCCggccttcc > 4:371781/1‑129 (MQ=255) ccGGTGCAAACCGCCGTGCTGAAACGCAACCTGGCCAACGCGCCCAC‑GTGG‑CCG‑GGCGGTTTTT‑GCAG‑CGCCGGC‑CCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCg < 3:387312/135‑1 (MQ=255) cGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCGTTT‑TGAG‑CCCCCCG‑CCCGCCGGCCTGAAAAAAAAACGCACGCAGACCCGGGTGTCCTCGTGTTTGAGGccggcct > 8:163137/1‑130 (MQ=255) aGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCTTTT‑TGAG‑CGCCCCG‑CCCGCCGCCATTAAAAAAAACGCCAGTCAGCCCCGGATTACCTCGTTTTTGAGGACGCCCTCATCTCACTATAGta > 6:361249/1‑134 (MQ=255) tgtaaaCGCACCTTGCCATAGGCGCCCA‑‑GGGG‑CTGTGGGGGCTTTT‑GGCC‑CGCCGGG‑CCCGCAGCCATAAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCg < 4:104919/132‑1 (MQ=255) cTGAAACGCAACCTGGCCTAAGGGCCCAC‑TTGG‑CGG‑GGGTGCTTTG‑GCCG‑CCCCGGG‑CCCGCCGCCTTTAAAAACAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCg < 6:171031/135‑1 (MQ=255) caCCCGGCCTAA‑CGCCCCCAGGGG‑CGG‑GGGGGGTTTT‑GCCCCCGCCGGC‑CCCGAACCAATTAAAAAACACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGc < 7:179103/134‑1 (MQ=255) gCCTAAGCGCCCAC‑GGGG‑CCG‑GGCGGCTTTT‑GCCG‑CGCCCGG‑CCCGCAGCCATAAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGAtgct < 8:95164/135‑1 (MQ=255) cccTACGCGCCCCG‑GGGG‑CGG‑GGCGGCTTTT‑GCCG‑CGCCCGG‑CCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGAtgct < 4:100192/134‑1 (MQ=255) ccatacgcgcCCAC‑GGGG‑CGG‑GGCGGTTTTT‑CCCG‑CGCCGGG‑CCCGCCGCCATAAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGAtgct < 8:84374/129‑1 (MQ=255) ccaatgcgcCCCC‑G‑GG‑CCG‑GGGGGCTTTTACCAG‑CCCCCGG‑CCCCCACCATTTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGAtgctg < 2:320268/130‑1 (MQ=255) tacgcgcCACC‑GGGG‑CCG‑GGCGGCTTTT‑CCAG‑CGCCCGG‑CCCGCAGCCATTAAAAAACACGCCAGTCAGACCCTGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCa < 4:41646/132‑1 (MQ=255) aGCCCCCAC‑GGGG‑CCG‑GGGGGATTTG‑GCCG‑CGCCCGG‑CCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCATc < 8:326553/135‑1 (MQ=255) gCAG‑CGCCCCG‑CCCGCCGCCATTTAAAAAAAAGCCAGTCAGACCCGGATTCCATCGTTGTTAAGGCCTCCGTGAACTCCCTATAGTACCCTGAAGGCCGATGCTGCATCGGTGTACCCAACTCACCTGGGCgggc > 4:267312/1‑134 (MQ=255) gatgggccggg‑cccgCTGCCATAAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCATCGGTGTACACAACTCAGCTGGGCggg < 4:381056/129‑1 (MQ=255) cccccgcCGGC‑CCCGCAGCCAATAAAAAACACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCATCGGTGTACACAACTCAGCTGGGCggg < 3:51800/130‑1 (MQ=255) gccgccccggccccGCCCCCATAAAAAAACACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCATCGGTGTACACAACTCAGCTGGGCggg < 5:361249/129‑1 (MQ=255) | ACTGGCGTGCGCGGGGTGCCAGCATGGTCGGCGGTTGCTGTGGCATCGGCCCGGAGCACATCGCCGAGCTGAAACGCAACCTGGCCTAAGCGCCCAC‑GTGG‑CCG‑GGCGGCTTTT‑GCAG‑CGCCCGG‑CCCGCAGCCATTAAAAAAAACGCCAGTCAGACCCGGATTCCATCGTTTTTCAGGCCTCCGTCATCTCCCTATAGTCCGCTGAAGGCCGATGCTGCATCGGTGTACACAACTCAGCTGGGCGGGA > KT2440/759303‑759551 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |