Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	1,567,244	C→T	7.0%	D48D (GAC→GAT)	CJ019_01477 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	1,567,244	0	C	T	7.0%	184.7 / 3.6	60	D48D (GAC→GAT)	CJ019_01477	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (32/22);  new base T (2/2);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

CGCTGGCATGCAAGTGCGCCGCGCAGTGCTGGGTGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCCGCGA  >  KT2440/1567124‑1567375                                                                                                                         |                                                                                                                                     cgCTGGCATGCAAGTGCGCCGCGCAGTGCTGGGTGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑cccgccc                                                                                                                       >  6:186408/1‑135 (MQ=255)             aGTGCGCCGCGCAGTGCTGGGTGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCcgcg                                                                                                           <  7:42537/135‑1 (MQ=255)                    cgcgCAGTGCTGGGTGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATAcc                                                                                                    >  5:196141/1‑135 (MQ=255)                    cgcgCAGTGCTGGGTGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATAcc                                                                                                    >  7:126057/1‑135 (MQ=255)                         aGTGCTGGGTGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGAGATCTGGC‑CCCGCCCGGGGCTGCCGCGCCATCCCCGccg                                                                                               >  5:219987/1‑133 (MQ=255)                         aGTGCTGGGTGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATCCCCGCAg                                                                                               >  2:332530/1‑135 (MQ=255)                         aGTGCTGGGTGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAg                                                                                               >  8:351991/1‑135 (MQ=255)                               ggggGATGCCCAGGGGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGAt                                                                                         <  6:34481/135‑1 (MQ=255)                                  tGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTCCCGCGCCAAACCCGCAGCCTGATCa                                                                                       >  3:363540/1‑134 (MQ=255)                                   gATGCCCACGTGGACCGCAGCCTGGTGAAGCTGCACGTCGTCAATGGCCAGTTACCTGAAATTAAGACCCGCCACCCCAGGGGGTACATAGGGA‑CCCCCCCCGGGCTTCCCCCCCCAACCCCCCGCCTGaaaca                                                                                      >  2:309623/1‑130 (MQ=255)                                   gATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGATATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCAcc                                                                                     >  6:305420/1‑135 (MQ=255)                                   gATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCAcc                                                                                     >  2:25083/1‑135 (MQ=255)                                   gATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACACGCCACGCCTGGGGTGATATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCAcc                                                                                     >  6:300358/1‑135 (MQ=255)                                        ccACGTGGCCCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGCTCACCATCGc                                                                                >  8:73903/1‑135 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATAccc                                                                                                   <  1:78150/116‑1 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATAccc                                                                                                   >  2:78150/1‑116 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGCTCACCATCGc                                                                                >  5:235045/1‑135 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCg                                                                                 >  3:275484/1‑134 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCg                                                                                 >  4:261451/1‑134 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCg                                                                                 >  3:60277/1‑134 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCg                                                                                 >  6:372121/1‑134 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCg                                                                                 >  7:972/1‑134 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCg                                                                                 >  1:29940/1‑134 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCg                                                                                 >  1:17821/1‑134 (MQ=255)                                        ccACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGCGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCg                                                                                 >  4:107743/1‑134 (MQ=255)                                             tGGACCGCAGCCTGGAGACGCGCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGc                                                                           <  1:222081/135‑1 (MQ=255)                                             tGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATa                                                                                                      >  5:118486/1‑108 (MQ=255)                                             tGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATa                                                                                                      <  6:118486/108‑1 (MQ=255)                                               gACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTg                                                                         >  8:266470/1‑135 (MQ=255)                                                   gCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGAAGCCTGATCACCATCGCCATGCTGATTg                                                                     >  4:76068/1‑135 (MQ=255)                                                    cAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATCCTGATTg                                                                     >  5:102808/1‑134 (MQ=255)                                                       ccTGGAGAAGCTCAACGCCTTCAATGGCGAGTTCCAGGAATTGATCACCCGCCACGCCTGGGGTGACGTCTGGA‑CCCGCCCGGGGCTGCCGCGCCCTGCCCGCAGCCTGAGCCCCCTCGCCCTGCTGATTGGCAt                                                                 >  8:345445/1‑135 (MQ=255)                                                            agaTGCTAAAGGACTTCAATGGCGTGTTCCAGTAAATGATCCCCGGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAAcc                                                            <  6:126962/135‑1 (MQ=255)                                                             gaAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAAcc                                                            >  7:260380/1‑134 (MQ=255)                                                                gCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCaa                                                        <  7:266470/135‑1 (MQ=255)                                                                ccTCAACTAATACATTGGGTAGTTACAGTAAATGATCACCCGCCAGGGATGGGGTGACTTCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATGGCCATGGTGATTGGCATGAACCGCaa                                                        <  5:286488/134‑1 (MQ=255)                                                                    aaCGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCTTACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAacgac                                                    >  8:377929/1‑135 (MQ=255)                                                                    aaCGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCAAGCTGATTGGCATGAACCGCAacga                                                     >  5:205923/1‑134 (MQ=255)                                                                        aCTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGc                                                >  1:62235/1‑135 (MQ=255)                                                                              aTGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAg                                           <  8:30016/134‑1 (MQ=255)                                                                                 gCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGc                                       >  6:387576/1‑135 (MQ=255)                                                                                  cGAGCTCCAGGAAATCCGCCCCCGCCACGCCTGGGGGGAAATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCCACGACGAGCTGAAGCTg                                        <  2:370610/133‑1 (MQ=255)                                                                                       tCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCtgc                                 >  8:266904/1‑135 (MQ=255)                                                                                            gAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCtgcgtgcg                            <  7:83343/135‑1 (MQ=255)                                                                                            gAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCtgcgtgcg                            <  4:389140/135‑1 (MQ=255)                                                                                              aaTGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTgcggc                          <  1:309623/135‑1 (MQ=255)                                                                                               aTGATCACCCGCCACGCCTGGGGTGACATCTGGACCCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTgcggc                          <  2:17821/135‑1 (MQ=255)                                                                                                         gCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATAcc                                                                                                    >  7:88632/1‑50 (MQ=255)                                                                                                         gCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATAcc                                                                                                    <  8:88632/50‑1 (MQ=255)                                                                                                          ccACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCGGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATgg              <  4:60277/135‑1 (MQ=255)                                                                                                              gCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTg          <  8:126057/135‑1 (MQ=255)                                                                                                                    ggggACATCTGGA‑CCCGCCCGGGGCTGCCGCACCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCcgc    <  5:214872/132‑1 (MQ=255)                                                                                                                    ggTTACATCTGGA‑CCCGCCCGGGGCTGCCGCTCCATACCAGCATCATGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACTAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCcgc    <  3:76068/135‑1 (MQ=255)                                                                                                                    ggTGATATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCcgc    <  5:300358/135‑1 (MQ=255)                                                                                                                    ggTGATATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCCCCATGCTGATAGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCcgc    <  5:305420/135‑1 (MQ=255)                                                                                                                    ggTGACTTCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCcgc    <  3:107743/135‑1 (MQ=255)                                                                                                                    ggTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCcgc    <  7:202091/135‑1 (MQ=255)                                                                                                                      tGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCCGcga  <  7:351991/135‑1 (MQ=255)                                                                                                                      tGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCCGcga  >  3:309877/1‑135 (MQ=255)                                                                                                                      tGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCCGcga  <  4:275484/135‑1 (MQ=255)                                                                                                                         |                                                                                                                                     CGCTGGCATGCAAGTGCGCCGCGCAGTGCTGGGTGATGCCCACGTGGACCGCAGCCTGGAGAAGCTCAACGACTTCAATGGCGAGTTCCAGGAAATGATCACCCGCCACGCCTGGGGTGACATCTGGA‑CCCGCCCGGGGCTGCCGCGCCATACCCGCAGCCTGATCACCATCGCCATGCTGATTGGCATGAACCGCAACGACGAGCTGAAGCTGCACCTGCGTGCGGCGGCCAACAATGGCGTGACCCGCGA  >  KT2440/1567124‑1567375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑