Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 1,720,951 T→G 33.4% D90A (GAC→GCC)  plsB ← Glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,720,9510TG33.4% 56.1 / 9.2 47D90A (GAC→GCC) plsBGlycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (19/11);  new base G (3/14);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 5.38e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGGGGTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGG  >  KT2440/1720817‑1721080
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ccGGGGTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGt                                                                                                                                    <  4:32747/135‑1 (MQ=255)
   gggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg                                                                                                                                 <  1:362036/135‑1 (MQ=255)
   gggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg                                                                                                                                 <  4:292074/135‑1 (MQ=255)
   gggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg                                                                                                                                 <  3:87229/135‑1 (MQ=255)
   gggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgg                                                                                                                                 <  7:154413/135‑1 (MQ=255)
    ggTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCggg                                                                                                                                <  5:361541/135‑1 (MQ=255)
     gTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgggg                                                                                                                               <  4:217893/135‑1 (MQ=255)
                 aaCACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTa‑gaa                                                                                                                   >  4:282706/1‑134 (MQ=255)
                  acacGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgggg                                                                                                                               <  3:218071/122‑1 (MQ=255)
                  acacGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCgggg                                                                                                                               >  4:218071/1‑122 (MQ=255)
                   cacGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑gaagaa                                                                                                                >  5:319967/1‑135 (MQ=255)
                       cTGACTGTATTGAGCGGCGCAGCTTCTGCGGCCGGCGGGCTGCCGGCAGCCACCAGGCGCGCGAGCGGGGGGGGGGCGCCGCTTTTGGCCGGGCGGCCGCGCCAGTCCGGGTGGGGGGTCAGGTA‑GAAGAAcgcg                                                                                                            <  4:304230/135‑1 (MQ=255)
                       cTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGCGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAAcgcg                                                                                                            <  6:319967/135‑1 (MQ=255)
                       cTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCGGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGGCCTGGCGCCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAAcgcg                                                                                                            <  2:48256/135‑1 (MQ=255)
                         gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACgcggc                                                                                                          >  8:26290/1‑135 (MQ=255)
                         gACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCGTTTGTACTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGCTA‑CAAGAAcgccgc                                                                                                          >  2:83018/1‑135 (MQ=255)
                              gAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGAGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGtt                                                                                                     >  8:252378/1‑135 (MQ=255)
                                       gcgcATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGCACGCGGCGGGTTCCTGCAGTg                                                                                            >  3:237773/1‑135 (MQ=255)
                                          cATCTTCTGCGGCATCCTGGCTGCCGGCCGCCACCAGGCGCGCGCGCGGGGGGGGGGCGCCGCTTTTGCCCTGGCGGCCGACCCCGTCGGGGGCGGGGGACAGTTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGc                                                                                         <  4:168823/135‑1 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGCGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGcc                                                                                        >  2:45266/1‑135 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGcc                                                                                        >  4:105732/1‑135 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCATGCAGTGGGcc                                                                                        >  4:150604/1‑135 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGGTCCTGCAGTGGGcc                                                                                        >  2:289277/1‑135 (MQ=255)
                                           aTCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGAGGGAGGGGCGCCGCTTTTTTACTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTTGGc                                                                                         >  5:361826/1‑134 (MQ=255)
                                                            ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGCGCCAGTCCGGGGCGGGGGTGCGGTT‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGCCGGCTACCGgcagcc                                                                       >  5:310220/1‑134 (MQ=255)
                                                            ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGCGCCAGGCCCGGGCGGGGGGCAGGTA‑GAAGAACGCGGCGGGGTCCTGCACTGGGCCGACGGCTACCCgccgca                                                                       >  5:119361/1‑135 (MQ=255)
                                                            ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGACCGCGGCGGGTTCCTGCAGGGGGCCGACGGCTACCGgcagca                                                                       >  1:263278/1‑135 (MQ=255)
                                                            ggctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCGGGCGGCCGCGCCCGGCCGGGGCGGGGGTCATGTA‑GAAGAAAGCGGCGGGGTCCTGCAGTGGTACGACGGCTACCGgcagca                                                                       >  7:312294/1‑135 (MQ=255)
                                                             gctgACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCAc                                                                      >  4:158333/1‑135 (MQ=255)
                                                                  cGGCAGCCACCCGGCGCGCGCGCGGGGGCGGGGCGCCGCTTTGGTCCGGGCGGCCGAGCCAGTCCGGGTCGGGTGTCAGGTA‑CAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGgc                                                                 <  7:184/135‑1 (MQ=255)
                                                                      aGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCgg                                                             >  7:199351/1‑135 (MQ=255)
                                                                           cccggCGCGCGGGGGGGGGCGGGGCGCCGCTGTGGCCCGGGCGGCGGAGCCCGGCGGGGGGGGGGGGC‑GGTACGACCACCGCGGCGGGTTCCTGCAGGGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGcc                                                        <  2:375244/132‑1 (MQ=255)
                                                                                       gCGTGGGCGGGGCGCCGCTTTTGTACTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGGA‑GAAGAACCCGGCGGGGTGCTGCCGTGGGCCGCCGGCTACCGGCAGCCCCGGGCGCGGCAGCCCCGCCATGGTGc                                            >  4:11605/1‑135 (MQ=255)
                                                                                            gggggggcgCCGCTTTTGCCCGGGCGGCCGCGCCAGGCCGGGGCGGGGGGCAGGTA‑GACGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCg                                       <  4:88494/132‑1 (MQ=255)
                                                                                             gcggggcgCCGCTTTTGTCCGGGCGGCCGAGCCAGTCCGGGGCGGGGGGCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGt                                      <  7:133142/135‑1 (MQ=255)
                                                                                             gcggggcgCCGATTTTGTCCTGGCGGCCGAGCCAGGGCGGGGCGGGGGGCAGGTA‑GAACCACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGt                                      <  8:330052/135‑1 (MQ=255)
                                                                                                 ggcgCGGTTTTTGGCCGGGCGGCCGACCCTGGCGGGGGGGGGGGCCTGGAA‑CACCACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTc                                  <  6:310220/135‑1 (MQ=255)
                                                                                                 ggcgCGGGTTTGGCCCGGGGGGCGGTGCCCGTCGGGGGCGGGGGGCCGGTA‑GACCACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTc                                  <  3:323035/135‑1 (MQ=255)
                                                                                                 ggcgCCGCTTTTGTCCTGGCGGCCGCGCCCGTCCGGGGCGGGGGGCCGGTA‑GAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTc                                  <  5:146131/135‑1 (MQ=255)
                                                                                                    gccgcTTTTGTCCTGGCGGCCGCGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGTGGCCGCCCCCCCGTGGTGCACTCGTGGTCgcg                               >  6:376563/1‑133 (MQ=255)
                                                                                                        cTTTTGCCGGGGGGGCCGAGCCTGCCGGGGGGGGGGGGCTGGTA‑GAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACg                           <  5:340334/135‑1 (MQ=255)
                                                                                                           ttGGCCGGGCGGCGGCGCCCGGCGGGGGCGGGGGTCAGGTA‑CACCACCGCGGCGGGGTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGc                         <  1:92068/134‑1 (MQ=255)
                                                                                                                     ggcCGCGCCTGGCCGGGGGGGGGGTCTGGTC‑GAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGa              <  8:258719/135‑1 (MQ=255)
                                                                                                                      gcCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTgcg             >  5:270859/1‑133 (MQ=255)
                                                                                                                      gcCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAg             >  3:232298/1‑135 (MQ=255)
                                                                                                                            ccAGGCCGGGGCGGGGGTATGGTA‑GAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCggg       <  4:119895/135‑1 (MQ=255)
                                                                                                                                 ccGGGGCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAgg  <  3:374852/135‑1 (MQ=255)
                                                                                                                                 ccGGGGCGGGGGCCAGGTA‑GAAGACCGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAgg  <  1:289277/135‑1 (MQ=255)
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CCGGGGTCTGGCCCCAGAACACGCTGACAGGAATGATCTGCGCATCTTCTTCGGCATGCTGGCTGACGGCAGCCACCAGGCGCTCGAGCGTGGGCGGGGCGCCGCTTTTGTCCTGGCGGCCGAGCCAGTCCGGGTCGGGGGTCAGGTA‑GAAGAACGCGGCGGGTTCCTGCAGTGGGCCGACGGCTACCGGCAGCACCGGGCGCGGCAGCCCCGCCTTGGTGCACTCGTGGTCGAGCACGGCCAGGTCGGTGAGCGCGGGCGAGG  >  KT2440/1720817‑1721080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑