| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 2,491,272 | A→C | 31.5% | H33P (CAC→CCC) | iutA → | Ferric aerobactin receptor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 2,491,272 | 0 | A | C | 31.5% | 18.3 / 4.9 | 33 | H33P (CAC→CCC) | iutA | Ferric aerobactin receptor |
| Reads supporting (aligned to +/- strand): ref base A (18/4); new base C (3/8); total (21/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.47e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
CCCATTCTCATTTACGACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACCA > KT2440/2491139‑2491406 | cccATTCTCATTTACGACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTAGGCCCCCTGCAc > 8:104587/1‑135 (MQ=255) tctcATTTACGACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATTCCATGATGTCTTTCAGTGCCCTGGCCCTGTTGCTCGGCCCCCTGCaacccgg > 2:246430/1‑129 (MQ=255) ttGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGGTCTTTTGACCATGCCGTGATGTGTTTCAGTGCCGTGGCCCGTGTGGTCCGCCCCCAGCCCC‑CCGGGGCCCCggcggcg > 2:291727/1‑134 (MQ=255) cAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGGTGTTTTGACCAAGCCTTTGTGTGTTTGAGTGGCGTGGGCCAGTTGAGCGGCCCCCCGCCCC‑CCGGGGCCGCGCCCGCGGCCg > 1:344346/1‑135 (MQ=255) aGCTAATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGGCGCGGTTATTTTCACCATGCCTTGATGTCTTGCTGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAg < 8:191738/135‑1 (MQ=255) tccTCACGCCCTTCCCCCGCCGGCGGGTTTCTTTTCACCTTGCCTTGATGTCTGGCAGGGCGGGGGCCCTGTGGCGCGGCCCCCTCCACG‑CCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGccc < 3:280430/130‑1 (MQ=255) aaGCCCTTGCACCGCCGTCGCGGGTGGTTTGACCCAGGCGTGGTGTGTTGCAGTGGCGGGGGCCTGTTGCTCGGCCCCCCGCCCC‑CCGGGGCCGCGCCGGCGGCCGCACACACCCCCCAGCAGGCCCGccccggc > 2:323266/1‑130 (MQ=255) aCCGCCGGCGCGGTTCTTTCCACCATGCCTTGATGTCTTGCCGGGCCGGGGCCCTGTTGCTCGGCCCCCTGCACG‑CCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGAcc < 4:352475/135‑1 (MQ=255) ggcggggTTATTTCCACCATCCTTGGAGGTCTTCCTGGGCGGGGGCCCGGTGGCGCGCCCCCCGCCCCG‑CGGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAg < 3:282144/130‑1 (MQ=255) aTGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCACCCGCAGCACCAgg > 6:378939/1‑135 (MQ=255) aGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGc > 7:502/1‑134 (MQ=255) ccGGGGCCCGGTTGCGCGCCCCCCCGCCCG‑CCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGAcc < 1:291727/135‑1 (MQ=255) tGGCCCTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCCACCGCTGGCCCAGGCGCTGACCACTTTCGCCGCCCCgg > 4:329552/1‑135 (MQ=255) cccTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGACCAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGCCCAGGCGCTGCCCACGTTCGCCGCCCCggccg > 1:311041/1‑135 (MQ=255) cccTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGCCCACTTTCGCCGACCccgccg > 8:86853/1‑135 (MQ=255) cccTGTGGCGCGCCCCCCTCCCCG‑CCGGGCCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccg < 1:10554/135‑1 (MQ=255) cTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGCCCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccggc > 7:304482/1‑135 (MQ=255) cTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCCGCTCGACCTGCCAGCCCAACCGCTGGCCCCGGCGCTGACCACTTTCGCCGCCCcggccggc > 7:70036/1‑135 (MQ=255) cTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCCACCGCTGGCCCAGGCGCTGCCCACTTTCGCCGACCAggccggc > 1:328920/1‑135 (MQ=255) tgtgGCGCGCCCCCCTGCCCG‑CCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAggccggcc < 8:54300/135‑1 (MQ=255) ttgcggggCCCCCTGCCCC‑CCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTg < 3:19987/129‑1 (MQ=255) ggcgcGGCCCCCGGCCCG‑CCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGc < 1:246430/131‑1 (MQ=255) tgctggCCCCCCGCCCGCCAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCTACCTGCCAGCCCAACCGCAGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGc < 7:41676/131‑1 (MQ=255) gCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCCGGTCCCGCTCGACCTGCCAGCCCCACCGCTGGCCCAGGCGCGGACCACTTTCGCCGCCCCGGCCGGCCGGCa > 7:156977/1‑135 (MQ=255) gCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGCCCACTTTCGCCGACCAGGCCGGCCTGCa > 1:227741/1‑135 (MQ=255) cgcGGCCCCCGCCCCG‑CCGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCAc < 8:37662/133‑1 (MQ=255) cTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCCGCCCAACCGCTGGACCAGGCGCTGACCACGATCCCCGCCCACCCcggccggccc > 3:262383/1‑133 (MQ=255) cTCGGCCCCCGCCACG‑CTGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCAc < 3:93475/135‑1 (MQ=255) cccccTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCACCCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACctgct > 8:110292/1‑135 (MQ=255) cccccTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACctgct > 6:159938/1‑135 (MQ=255) cccccTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACctgct > 2:19791/1‑135 (MQ=255) ccTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGCCCAGGCGCGGCCCACTTTCGCCGCCCAGGCCGGCCTGCCCCTGCGGTa > 2:325491/1‑135 (MQ=255) ccTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGt > 3:173259/1‑134 (MQ=255) tGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCCCTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTaca > 2:324022/1‑135 (MQ=255) aCG‑CAGGGGCCGCGCCGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCCACCGCTGGACCCGGCGCTGCCCACTTTCGCCGCCCCGGCCGGCCGGCACCGGCGGTAcgccc > 1:327430/1‑134 (MQ=255) | CCCATTCTCATTTACGACTTGTCAGGAAGCTCATTCGTGCCTCTGATGCTCAAGCCCTTGCACCGCCGTCGCGGTTCTTTTCACCATGCCTTGATGTCTTGCAGTGCCGTGGCCCTGTTGCTCGGCCCCCTGCACG‑CAGGGGCCGCGACGGCTGCCGAACAGACCCAGATGCAGGCCCGCCAGGTCCAGCTCGACCTGCCAGCCCAACCGCTGGACCAGGCGCTGACCACTTTCGCCGACCAGGCCGGCCTGCACCTGCTGTACACCA > KT2440/2491139‑2491406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |