Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,245,145	A→C	55.7%	L178R (CTG→CGG)	CJ019_03982 ←	Crotonyl‑CoA hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,245,145	0	A	C	55.7%	3.2 / 26.0	77	L178R (CTG→CGG)	CJ019_03982	Crotonyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  ref base A (10/16);  new base C (25/14);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 7.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTG  >  KT2440/4245022‑4245276                                                                                                                             |                                                                                                                                    cgTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGCCCCCCCCGG‑CGGCCCCGGCGCGCTCg                                                                                                                          >  2:243042/1‑135 (MQ=255)           gCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAAATGCTCGTCGGCGCAGACTTCGCTGAACAACCCCG‑CGGCCAGGGCGCGATCGGCGccccccc                                                                                                                >  4:80357/1‑132 (MQ=255)               ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGCGCCCcaccacac                                                                                                            >  6:118742/1‑133 (MQ=255)               ggTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGCGCCCGATACGCGCCCCCCAAcag                                                                                                            >  2:52525/1‑133 (MQ=255)                gtgggcgaATCGGCGATGGTGGGGCCTTGGGCCAGGGGCCGGGCATGTGCGGCGCAAACGGCAGGAAAGTGCTCGGCGGCCAAGACTACGGTGACCAACCCGG‑CGGCCTGGGCGCGAGGGGCGCCCCACAACtcg                                                                                                           <  2:17082/131‑1 (MQ=255)                gTCTGCGCATCGGCGATGGGCGGGCCATTGGCCAGGCGCCCGGCCAGGTCGGCGGCACGGGCAGGCAACTGCGCGGCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCTATGGGCGCCCCACAACtcg                                                                                                           <  6:180632/135‑1 (MQ=255)                      gcATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAg                                                                                                      >  1:267474/1‑134 (MQ=255)                        aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGTCGCGGa                                                                                                    >  1:310814/1‑134 (MQ=255)                        aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAggta                                                                                                   >  4:317907/1‑133 (MQ=255)                        aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGAAACGCGCCCCCCAACGCGTCGAGGa                                                                                                    >  3:115615/1‑134 (MQ=255)                        aTAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCCGGCGCGATCCGCGCCCCACAACTCGTCGAggc                                                                                                    >  3:82670/1‑133 (MQ=255)                          tggCGATGGTGGGGCAATTGGCCTGGCGCGCGGCTTGTGCGGCGGCAACGGCAGGCAAGTGGTGGTCGGCGCAGAGTGCGCTGACCAACCGGG‑CGGCCTGGGGGCGATCGGGGCCCCACAACTCGTCGAGGAAca                                                                                                 <  7:28374/134‑1 (MQ=255)                          aGGCTACGGGCGGGCCATGGCCCAGGCGCCCGGCCTGTTCGGCGGCAACGGCAGGCACCTGCGCGGCGGCGCAGACTTCGCTGACCACCCGGG‑CGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                 <  4:98343/135‑1 (MQ=255)                          aGGCGATGGTCGGGCCTTTGGCCTGGCGCCGGGCCTGTTCGGGGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                 <  2:251595/135‑1 (MQ=255)                          aGGCGATGGTCGGGCCATGGGCCCGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                 <  8:347466/135‑1 (MQ=255)                           ggCGTAGGTCGGGCGATTGGCCTGGGGCGGGGCCTGTGCGGGGGCAAGGGCTGGCAAGTGCTCGTCGGCGCTGACTTGGCTGACCACCCGGG‑GGGCCTGGGCGGGATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                 <  8:315983/134‑1 (MQ=255)                           ggCGACGGGCGGCCCATGGCCCAGGCGCCCGGCCAGTGCGGCGGCACCGGCAGGCAACGGCGCGGCGGCGCAGACTGCGCTCACCACCCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                 <  1:243042/134‑1 (MQ=255)                           ggCGAAGGGGGGGCAATTGGCAAGGCGCCCGGCCTGTTCGGCGGAAACGGCTGGCAACTGCGCGTCGGCGCTGACTTGGCTGACAAACCCGG‑CGGCCAGGGCGCTATCGGCGCCCCACAACTCGTCGAGGAAca                                                                                                 <  6:381950/134‑1 (MQ=255)                             cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCAACACGTCGAGGAAcagca                                                                                              >  1:266128/1‑135 (MQ=255)                             cGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGCTCGGCGCCCCCCCACGCGTCGAGGAAcagca                                                                                              >  8:1026/1‑135 (MQ=255)                              gAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGCCTTCGCTGACCACCCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACCACACGTCGAGGAAcagcag                                                                                             >  6:196136/1‑135 (MQ=255)                                  gTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCACGGGGCGCTCGGCGCCCCCCAACGCGTGGCGGGACACCAGgcgc                                                                                         >  1:230545/1‑135 (MQ=255)                                     gggCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTg                                                                                      >  8:372564/1‑135 (MQ=255)                                       gCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACACGTCGCGGCACCCCACgcgcgttgc                                                                                    >  4:385698/1‑130 (MQ=255)                                         cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGGACAGCAGGCGCTTGGCCt                                                                                  >  5:184465/1‑135 (MQ=255)                                         cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGAt                                                                                                                            >  8:244819/1‑93 (MQ=255)                                         cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGGACAGCCGGCGCTTGGCCt                                                                                  >  8:254060/1‑135 (MQ=255)                                         cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCCGGCGCGCTAGGCGCCCCACAACTCGTCGAGGCACAGCAGGCGCGTGGccg                                                                                  >  4:340003/1‑134 (MQ=255)                                         cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCCGGCGCGATCGGCGCCCCCCAACGCGGCGAGGCACAGCCGGCGCTTGGCCt                                                                                  >  4:118480/1‑135 (MQ=255)                                         cATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGAt                                                                                                                            <  7:244819/93‑1 (MQ=255)                                               cccggCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACTCGGCGAGGCACCGCCGGCGCTTGGCCTGCTCGc                                                                            >  3:153506/4‑135 (MQ=255)                                               ccAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCAAGGCGCCCCCCCCCGCGTGGAGGCACACCCGGCGCGTGGCCGGCTCGc                                                                            >  1:10443/1‑135 (MQ=255)                                                      cccGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCACGGCGCGCTCGGCGCCCCCCCACACGTCGAGGCAAAGCCGGGGCGTTGGCGGGGCGCTGCCGa                                                                      >  1:14380/1‑134 (MQ=255)                                                           ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCCACACGTCGGGGGCCCCCCGGCGCGTGGCCGGCGAGCAGCCGaaagagc                                                                >  2:311072/1‑129 (MQ=255)                                                           ccAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGCACAGCAGGCGCTTGGCCTGCTCGCTGCCCATCCGcc                                                                >  6:56383/1‑135 (MQ=255)                                                             tgtgcggcggCACCGGCCGGAAACTGCGCGGCGGCGCAGAGTTCGCGCACCACCCCGG‑CGGCCAGGGCGGGAGCGGGGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                              <  6:349770/131‑1 (MQ=255)                                                             aGTGCGGCGGCAACGGCAGGCAACTGCTCGGCGGCGCAGACTTCGCTCACCAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGt                                                              <  7:327776/135‑1 (MQ=255)                                                                       cAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGCTCGGCGCCCCCCAACTCGTCGCGGAACACCAGGCGCGTGGCCTGCTCGCGGCCGCTCACCCGGGGCCGGTGcc                                                    >  7:286057/1‑135 (MQ=255)                                                                        aaCGCCAGCCAACTGCGCGGCGGCGCAGACTGCGCGCACCAACCCGG‑CGCCCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCa                                                   <  8:118502/135‑1 (MQ=255)                                                                            ggaggcaACTGCTCTCCGGCGCAGAGTTCGCTGACCACCCGGG‑CGGCCAGGGCGCGTGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTg                                               <  7:135587/133‑1 (MQ=255)                                                                            gcaggcaACGGCGCGGCGGCGAAGAGTGCGCTCACCACCCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTg                                               <  7:182431/135‑1 (MQ=255)                                                                              agccaACTGGTGGCCGGCCCCGACTGCGGCGACCACCCGGG‑CGCCCGGGGCGGGATCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCATGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGgc                                             <  4:15193/132‑1 (MQ=255)                                                                                     tGCTCGTGGGCGCAGACTTCGCTCACCAACCCGG‑CGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                      <  2:298144/135‑1 (MQ=255)                                                                                     tGCTCGTCGCCGCAGACTGCGCTCCCCAACCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                      <  2:10443/135‑1 (MQ=255)                                                                                     tGCGCGGCGGCGCAGACGTCGCGGCCCAACCGGG‑CGGCCGGGGCGCGCGGGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCa                                      <  7:310588/135‑1 (MQ=255)                                                                                        tcgtcgGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCg                                   >  8:231617/1‑135 (MQ=255)                                                                                          ggcgGCGCAGAGTGCGCAGACCACCCCGG‑CGGCCTGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                                 <  3:24887/133‑1 (MQ=255)                                                                                          ggcgGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCggg                                 <  6:279877/133‑1 (MQ=255)                                                                                            cgGCCCAGACTGCGCTGCCCAACCCGG‑GGGCCCGGGCCGGTGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCGCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGc                               <  5:118742/135‑1 (MQ=255)                                                                                             ggCGCAGACTTCGCTGACCACCCCGG‑CGGCCCGGGCGCGATCGGCGCCCCACAACACGTCGAGGAACAGCACGCGCTTGGCCAGCTCGCGGCCGCTCACCCGTGGCAGGGGCCAGCAGGCGCCGGCCGCGGGGcg                              >  6:386376/1‑134 (MQ=255)                                                                                             ggCGCAGACTCCGCTGCCCACCCCGC‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGCGGAACAGCAGGCGCGTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                              <  5:372516/135‑1 (MQ=255)                                                                                               cgcAGACTTCGCTGACCACCCCGG‑GGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                            <  4:108344/135‑1 (MQ=255)                                                                                               cgcAGACTTCGCTGACCACCCCGC‑CGGCCCGGGCGCGATCGGCGCCCCCCAACACCGCCAGGCACCCCCGGCGCATGGCCAGCTCGCAGCCCATCAGCCGTGGCACGTGGCCGCGTGCGCCCGCATCGGGGcg                              >  2:256423/1‑132 (MQ=255)                                                                                               cgcAGACTTCGCTGACCAACCCGG‑CGGCCCGGGCGCGATCGGGGCCCCACAACTCGGCGAGGAACATCAGGCGCTTGGCCTGCTCGCTGCCGAGCCGCCCGGGCCGGTGCCCGCTGGCGCCGGCCTCGGGGCt                              >  1:26581/1‑133 (MQ=255)                                                                                               cgcAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGCTCGGCGCCCCACAACTCGGCGAGGAACAGCCGGCGCTTGGCCTGCTCGCTGCCGCTCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCt                              >  7:253603/1‑133 (MQ=255)                                                                                               cgcAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGt                            <  2:310814/135‑1 (MQ=255)                                                                                                gcAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                           <  4:201589/135‑1 (MQ=255)                                                                                                gcAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                           <  6:93587/135‑1 (MQ=255)                                                                                                ccAGACTGCGCGCCCCACCCGGG‑CGGCCGGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTa                           <  8:336471/134‑1 (MQ=255)                                                                                                    aCTTCGCTGCCCAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                       <  6:193069/135‑1 (MQ=255)                                                                                                    aCTTCGCTGACCACCCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                       <  6:162406/135‑1 (MQ=255)                                                                                                    aCTTCGCTGACCAACCCGG‑CGGCCCGGGCGCCATCGGCGCCCCCCCACTCGTCGAGGCACAGCAGGCGCTTGGCCGTCTCCCTGCCGATCAGCCCGGGCAGGTGCCCGCTGGCGCCGGCCTCGGGGCGGGAGccc                       >  7:101359/1‑135 (MQ=255)                                                                                                    aCTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGCTCGGCGCCCCCCAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCCTCGGGGCTGTTGccc                       >  3:105066/1‑135 (MQ=255)                                                                                                    aCTGCGCTCACCCCCCCGG‑CGCCCGGGGCGCGCGGGGCCCCCCACAACTCGTCGAGGAACAGCCGGCGCTTGGCCGGCGCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                       <  6:371871/135‑1 (MQ=255)                                                                                                    aCTCCGCTCACCACCCGGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGccc                       <  3:385698/135‑1 (MQ=255)                                                                                                           tGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGTACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCggg                >  5:141105/1‑135 (MQ=255)                                                                                                            gACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCCCAACTCGTCGCGGGACACCCGGCGCTTGGCCGGCTCGCTGCCGCTCACCCGGGGGCGGTGCCAGCGGGCGCCGGCATCGGGGCTGTAGCCCAAGCTggg               >  4:251237/1‑134 (MQ=255)                                                                                                              ccccgccGG‑CGGCCCGGGCGGGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt             <  3:228059/130‑1 (MQ=255)                                                                                                              cccaCCGGG‑GGGCCCGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCATCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt             <  2:3232/132‑1 (MQ=255)                                                                                                              ccACCCCGG‑CGGCCCGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt             <  7:81388/135‑1 (MQ=255)                                                                                                              ccACCCCGG‑CGCCCCGGGCGCGAGCGGCCCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt             <  3:278206/135‑1 (MQ=255)                                                                                                              ccAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt             <  2:266128/135‑1 (MQ=255)                                                                                                              ccAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt             <  2:322251/135‑1 (MQ=255)                                                                                                              ccAACCCGG‑CGGCCAGGGCGCGAGCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt             <  6:184465/135‑1 (MQ=255)                                                                                                              ccAACCCGG‑CGACCTGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt             <  8:215704/135‑1 (MQ=255)                                                                                                               ccaCCCGGCCGGCCTGGGCGCGAGCGGCGCCCCACAACTCGGCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGgtgt             <  2:157837/133‑1 (MQ=255)                                                                                                                          gcCAGGGCGCGACCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTg  <  3:354927/135‑1 (MQ=255)                                                                                                                             |                                                                                                                                    CGTATCAGCCGCTTGGTCTGCGCATAGGCGAAGGTCGGGCCATTGGCCAGGCGCCCGGCCAGTTCGGCGGCAACGGCAGGCAACTGCTCGTCGGCGCAGACTTCGCTGACCAACCCGG‑CGGCCAGGGCGCGATCGGCGCCCCACAACTCGTCGAGGAACAGCAGGCGCTTGGCCTGCTCGCTGCCGATCAGCCGTGGCAGGTGCCAGCTGGCGCCGGCATCGGGGCTGTAGCCCATGCTGGTGTAGCCGGCCTTG  >  KT2440/4245022‑4245276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑