| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,258,554 | A→C | 20.5% | H41P (CAC→CCC) | pht2 → | Phthalate 4,5‑dioxygenase oxygenase reductasesubunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,258,554 | 0 | A | C | 20.5% | 147.0 / 3.6 | 85 | H41P (CAC→CCC) | pht2 | Phthalate 4,5‑dioxygenase oxygenase reductasesubunit |
| Reads supporting (aligned to +/- strand): ref base A (21/43); new base C (19/2); total (40/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.14e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGC > KT2440/4258423‑4258686 | aaCAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCAcca > 7:192130/1‑134 (MQ=255)aaCAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCt > 4:311696/1‑135 (MQ=255) cAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGc > 8:232033/1‑135 (MQ=255) ccGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCg > 4:190618/1‑135 (MQ=255) cGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGACCTGc > 7:155098/1‑131 (MQ=255) cGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGc < 8:155098/131‑1 (MQ=255) aCATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACg < 7:13431/135‑1 (MQ=255) aCATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACg > 4:53652/1‑135 (MQ=255) tGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGc < 4:25513/135‑1 (MQ=255) aTCGATGCCGTCGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTg < 2:358958/135‑1 (MQ=255) cgctGCCGTCGTGGTATCCAGTAACGATGAAGCGAAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGt < 2:361585/132‑1 (MQ=255) aTGCCGTAGTGTTAACCGTTAACTTTGACGCGCATGTTATCTGCAGCTTCGAGCTGGCCGGGGCAGATGGCAGCCTGATGCCGGCGTTCTGCGCCGGCGCCCATATCGACGGGCACCTGCCCGACGGGCTGGTgc < 2:159528/135‑1 (MQ=255) aTGCCGTAGTGGTATCCCGTAACGATGACGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCGGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTgc < 4:342001/135‑1 (MQ=255) aTGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTgc < 3:146182/135‑1 (MQ=255) aTGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTgc < 4:143703/135‑1 (MQ=255) ccGTAGTGGTATCCCGTAACGTTGCAGCGCAGGGTATCTGCAGCTTCGAGCTGGCGGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGcc < 5:320375/135‑1 (MQ=255) tAGTGGTATCCCTTAACGATGTCGCGCTGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGAGGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGt < 7:383252/135‑1 (MQ=255) tAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGt < 7:134166/135‑1 (MQ=255) tGGTATCCCTTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTAt < 5:146254/134‑1 (MQ=255) tGGTATCCCGTACCGATGACGCGCAGGGTAGCTGCAGCTTGGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCGGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACGGGCTGGTGCGCCAGTAt < 5:59960/134‑1 (MQ=255) tGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACGGGCTGGTGCGCCCGTa > 3:344127/1‑133 (MQ=255) tGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCAGCCCGACCGGCGGGTGGGCCacgatt > 8:189163/1‑130 (MQ=255) ggTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCAGGTGCGCCAGTATTc > 2:240814/1‑135 (MQ=255) gTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCg < 2:145351/135‑1 (MQ=255) tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCCACGGGCGGGTGCGGCAGTATTCGcgg > 4:6590/1‑133 (MQ=255) tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCCACGGGCTGGTGCGGCAGGATTCGCtg > 4:100444/1‑135 (MQ=255) tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCGACGGGCTGGTGCGCCAGTATTCGCtg > 1:5665/1‑135 (MQ=255) tCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCCCCCGGCGGGTGGGCCAGTATTAGCtg > 4:266169/1‑135 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCAGCCCCCCCGGCGGGGGCGCCCGGATTAGGtgt‑g > 1:277756/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACGGGCTGGTGCGCCCGTATTAGCtgt‑g > 8:121633/1‑134 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCCACGGGCTGGTGCGCCAGTAATAGCtgtt > 7:383169/1‑133 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCtgt‑g < 8:273646/134‑1 (MQ=255) cGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCATCCCCACAGGCTGGTGCCACAGGATTAGCtgtt > 7:23734/1‑133 (MQ=255) tAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCGGCCCGACGGGCGGGTGCGCCCGTATTAGCTGT‑GGaa > 4:360741/1‑135 (MQ=255) ccGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCGGCGGGAGTTGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACGTGCGCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAacc < 6:120016/134‑1 (MQ=255) cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCGGCCCGCCCGGCGGGTGCGCCCGGATTAGCTGT‑GGAccca > 1:277984/1‑135 (MQ=255) cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCAGCCCGACCGGGGGGTGGGCCCGTATTAGCtgt‑ggcccca > 6:5182/1‑129 (MQ=255) cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCAGCCCCACGGGCTGGTGCGCCACTATTCGCggt‑ggacacc > 7:284213/1‑129 (MQ=255) cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTGGCGGT‑GCCaccc > 2:278168/1‑134 (MQ=255) cGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGACCGCCGGGGTGGTGCGCCAGTATTAGCTGT‑GCCaccc > 1:230509/1‑134 (MQ=255) aaGCGCAGGGTATCTGCAGCTTCGAGCTGGACGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCAGGCCCCCCGGCGGGTGGGCCCCTTATCGCGGT‑GCCACcccccc > 2:293420/1‑134 (MQ=255) aGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCg < 7:7880/134‑1 (MQ=255) gcgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCCGCCCCCCCGGCGGGTGCGGCCGGATTAGCTGT‑GCCAAcccccc > 2:287586/1‑132 (MQ=255) cgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGGCCCACCGGCGGGTGCGCCACTTTTAGCTTTGGCACCCAccccaa > 2:345202/1‑132 (MQ=255) cgcAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCAGCCCCACGGGCGGGTGGGCCCCTATTAGCTGT‑TCAACcccccccca > 2:281542/1‑131 (MQ=255) aGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCCGCCCCACCGGGAGGTGGGCCAGTGTTAGGAGT‑GGCACCCCCCCCAAGAAc > 8:57742/1‑135 (MQ=255) ggTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGc < 5:69516/135‑1 (MQ=255) ggTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACCTGCACCTTCCCGCCCGGCAGGTGCGCCACTAATAGCTGT‑GGAAACAACCCCAAGAAAGc > 4:260795/1‑135 (MQ=255) gTATCTGCAGCTGCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGc < 1:163737/134‑1 (MQ=255) gCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGACGGGCTGGGGCGGCCGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGcg > 4:186288/1‑134 (MQ=255) aGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGc < 3:260795/132‑1 (MQ=255) tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCCGCCCCCCGCGCGGGGGGGCCAGTATTAGCGGT‑GGACACACCCCGCAGAACCCCCACGCGATCTgc > 4:197672/1‑134 (MQ=255) tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACATGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGa > 7:366833/1‑135 (MQ=255) tCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGCCGTTCCGCGCCGGCGCCCATATCGACGTGCCCCTGCCCCCCGCGCGGGTGCGGCCGGATTCCCGCT‑GCACCCCCCCCCCAGCACGCCCTCGCCATCTGa > 4:50872/1‑135 (MQ=255) cGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCCCCTGCCCGCCGGGCTGGGGCGCCCCTTTTAGCTTT‑GGACCCACCCCCAAGAACGCCATCGCGATCTggt > 8:101589/1‑133 (MQ=255) gAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGCCGTGCACCTGCCCGTCGGGCTGGTGCGCCAGTATTAGCTGT‑GCAACCACCCCGGAGAACGGCATCGCTATCTGAtt > 4:116128/1‑135 (MQ=255) gAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGGTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGa < 5:383398/133‑1 (MQ=255) aGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGAtt < 7:244929/134‑1 (MQ=255) aGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTg < 8:161210/135‑1 (MQ=255) aGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTg < 4:118538/135‑1 (MQ=255) aGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTg < 5:238695/135‑1 (MQ=255) gCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGGGGGTGGGCCAGTATTCGCTGT‑GCAACCCCCCCGCAGAACGCCCTCGCTATCTGAATgg > 4:316411/1‑135 (MQ=255) cgcgGCAGATGGCAGCCTGCGGCCGGCGTTCAGCGCCGGCGCCCATATCGACGGGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACt < 1:43949/135‑1 (MQ=255) ccgATGGCCGCGGGCGGCCGGGGTTCCGCGCCGGCGCCCATATCGACGGGCCCCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACg < 2:189925/133‑1 (MQ=255) cAGAGGGCAGCCTGCTGCCGGCGTGCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGACCGCCATCGATATCTGATTGGCGAACTCAACg < 6:82936/135‑1 (MQ=255) gctgcCGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCGGCCCGCCCGGCGGGTGGGGCCGTATTAGCGGT‑GGCACCCCCCCCCAGAACCCCAACGCGATCTGATTTGCGGTCTCAACGACCCCGCGTcgcg > 2:14657/1‑135 (MQ=255) ctgcCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgg < 4:236186/135‑1 (MQ=255) tgcCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTcgcg < 4:355618/133‑1 (MQ=255) tgcCGGCGTTCAGCGCCGGCGCCCATATCGACGGGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCggg < 1:293420/135‑1 (MQ=255) gcCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 2:277756/135‑1 (MQ=255) gcCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 7:170099/135‑1 (MQ=255) gcCGGCGTGCAGCGCCGGCGCCCAACTCGACGGGCACCAGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 3:356472/135‑1 (MQ=255) cGGCGTTCAGCGCCGGCGCCCATATCGACGTGGCCCGGCCCGCCCGGCGGGGGGGCCCGTATTTGCGGT‑GGAACCCCCCCCCAGAACGCCCTCGCGTTCGTCTTTGGGGACTCCACGACCCCGCGTCGCgggggg > 8:178707/1‑133 (MQ=255) cGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGTTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 2:28652/133‑1 (MQ=255) cGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 8:254609/133‑1 (MQ=255) cGGCGTGCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCgggg < 4:112003/133‑1 (MQ=255) aGCGCGGGCGCCCATATCGCCGTGCACCGGCCCGCGGGGCGGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGt < 5:200350/135‑1 (MQ=255) aGCGCGGGCGCCAATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCTGTATTCGCTGT‑GCAACCACCCCGAATAACGCCATCGCTATCTGATTGGCGTAATCAACGACCCGGCTTCGCGGGGCGGTTCTCg < 1:345202/134‑1 (MQ=255) ccGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTATcc < 3:227375/135‑1 (MQ=255) gcCCTTATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc < 1:345442/135‑1 (MQ=255) gcCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc < 3:186288/135‑1 (MQ=255) gcCCATATCGACGGGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCAGCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc < 8:276156/135‑1 (MQ=255) cccATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCGATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCAc > 7:269719/1‑134 (MQ=255) tGCACCTCCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAg < 3:190618/135‑1 (MQ=255) gCACCTGCCCGCCGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCCAACAACGCCAACGCTATCTGATTGGCGTACACAACGACCCCGCTTCGCCGGGCGGTTCTCGTAGCCAGCACGAACAGGTGCaacc > 8:281622/1‑133 (MQ=255) | AACAGCCGACATGATCGATGCCGTAGTGGTATCCCGTAACGATGAAGCGCAGGGTATCTGCAGCTTCGAGCTGGCCGCGGCAGATGGCAGCCTGCTGCCGGCGTTCAGCGCCGGCGCCCATATCGACGTGCACCTGCCCGACGGGCTGGTGCGCCAGTATTCGCTGT‑GCAACCACCCCGAAGAACGCCATCGCTATCTGATTGGCGTACTCAACGACCCGGCTTCGCGGGGCGGTTCTCGTAGCCTGCACGAACAGGTGCAAGC > KT2440/4258423‑4258686 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |