| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,626,159 | C→A | 19.9% | intergenic (+117/+181) | CJ019_04367 → / ← pgsA | hypothetical protein/CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,626,159 | 0 | C | A | 19.9% | 62.4 / 2.2 | 38 | intergenic (+117/+181) | CJ019_04367/pgsA | hypothetical protein/CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (20/9); new base A (2/6); total (23/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGT > KT2440/4626025‑4626281 | aGCGATATGTCCCGGTAGCCCCCGGCGTCACGGCCAGGGCGCCGGGTTGTGGTGTTTGAACTTGAATTTCGTCGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTc < 8:6589/135‑1 (MQ=255) ccAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTTCTTGAATTTCGTAGAGGGGGGGGTATTGAGGCAGAATGCATTCGAACCAAAAAA‑GGCCCCG‑AAGGGCCGTTTTTCAACGACTTa > 3:31528/1‑134 (MQ=255) ccAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACtt > 5:130825/1‑133 (MQ=255) ccAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGGTTGTTCTTTAAATTAGGAGAGGGCGGGGGATTGAGGCCGAATGCATTAGAACGCAAAAA‑AGCCCCG‑AAGGGGCGTTTTTAAACCACGTAc > 5:271286/1‑135 (MQ=255) ggTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAgc > 2:45570/1‑134 (MQ=255) ggTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGa < 7:100425/135‑1 (MQ=255) tcGGCGTCACTGCCAGGGAGCCGGGTGGTGTTGTTTGTACTTTATTTTCGTTGTGGGCGGGGTATTGAGGCAGATTGCTTTCGAACGAAAAAT‑GGCCCCT‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACt < 4:260008/134‑1 (MQ=255) aCGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTTGTTGAATTTGGTATAGGGCGGGGTTTTGAGGCAGAATGCATTGGAACGAAAAAA‑GGCCCCG‑AAGGGGCTTTTTTCCACGACTTACAGACTTCGAc > 8:10304/1‑134 (MQ=255) aCGGCGCCACGGCCAGGGGGCCGCGTTGTGGTGTTTGTTCTTGAATTGCTTCCAGGGCGGGGTTTTGCGGCAGAATCCATTCGAACCAAAAAA‑GCCCCCG‑AAGGGCCTTTTTTAAACGACTTACAGACTTCGACt < 7:10304/135‑1 (MQ=255) cGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTTCTTGTATTTGGTTGAGGGGGGGGTTTTGAGGCCGAATGCCTTCGAACGAAAAAA‑GGCCCCG‑CAGGGCCGTTTTTGAACGACTTAAAGACGTCGGCTGaaa > 1:361242/1‑135 (MQ=255) cACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCt < 8:274415/135‑1 (MQ=255) gccgcGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTAt > 1:2176/1‑135 (MQ=255) gccgcGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTAt > 7:118198/1‑135 (MQ=255) ggtttGGGGTGTTTGTACTTTAATTTCGTTGCGGGCGGGGTATTGAGGCAGAATCAATTCGAACGAAAAAC‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTg < 2:139198/132‑1 (MQ=255) gTTGTGGTGTTTGTACTTGAATTTAGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACCAAAAAA‑AGCCCCG‑AAGGGCCTTTTTTAAACGCCTTACATACTTCGACTGAAACCGGTAGATCATTATTTTGa > 6:59506/1‑135 (MQ=255) ggtgTTTGTACTTGAATTTAGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTAAAGACTTCCACTGAAACCATTAGAACTTTATTTTGAGCgg > 7:295604/1‑134 (MQ=255) tGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑TGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCTACTGAAACCTGTAGATCTTAATTTGGACCGGGAAACGAGACTc > 1:272745/1‑134 (MQ=255) tGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTc > 4:148890/1‑134 (MQ=255) aaTTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGAGCTTTATTTGGAGCGGGCAACGAGCCTCGaa > 4:19304/1‑135 (MQ=255) gggCGGGTTATGCAGCCATAATCAATCCTAACGAAAAAC‑GCCCCGG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTCAACCCTGAAGATCTTAATTTGCCGCGGGAAACGAGACTCGAACTCGCGAcccc < 6:269504/135‑1 (MQ=19) gggCGGGGT‑TTTAGGCCACATGAATTGCAACCAAAAAACGCCCCCC‑ACGGGCTTTTTTTCAAGGAGTTACACACTTCGACTGAACCCTTTAGATCTTTATTTGGAGCGGGAACCGCGACTCGAACTCGCGAcccc < 8:46603/135‑1 (MQ=16) gggCGGGATATTCCGCCAGAATCCATGCCAAC‑AAAAAA‑CGCCCCGTAAGGGCCTTTTTTAAACCACTAACACACTACGACTGAACCCTGTAGATCTTTATTGGGCGCGGCAAACGATACTCGAACTCGCGAcccc < 8:54553/135‑1 (MQ=255) ggggTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGTGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTACATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGa > 4:28331/1‑133 (MQ=255) ggggTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGAcc > 3:58540/1‑135 (MQ=255) gcctaaTGAATTCTAACAAAAAAC‑GGCCCCT‑ACGGGCCTTTTTTCAACTACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGCGCGGGAACCGAGACTCCAACTCGCGACCCCGACCTTGGCAAGGTc < 7:26515/131‑1 (MQ=25) aaTGAATTCTAACGAAAACA‑GCCCCCG‑ACGGGCGTTTTTACAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGCAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGc < 7:119213/135‑1 (MQ=25) tGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACTACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGct > 8:318700/1‑134 (MQ=255) tGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGct > 4:51166/1‑134 (MQ=255) cATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTa > 5:278737/1‑135 (MQ=255) cATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCACGACCTTGGCAAGGTCGTGCTCTa > 1:13075/1‑135 (MQ=255) aTTCGAACCAAAAAA‑GCCCCCG‑ACGGGCATTTTTTAAACCACTTACAGACTTCGACTCAAACCTGTAGAGCTTTATTTGGAGCGGCAACCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc < 8:79373/135‑1 (MQ=21) aTTCCAACAAAAAAC‑GCCCCCG‑AGGGGCTTTTTTTAAACGACTTACAGACTGCGACTGAAACGTGTAGATCTTTATTTGGCGCGGCAAACCAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTAc < 2:2176/135‑1 (MQ=21) gaacgaaAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGCCCTTGGCAAGGTCGTGCTCTACCAAc > 5:307730/1‑135 (MQ=255) gaacgaaAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAAc > 3:124736/1‑135 (MQ=255) gaacgaaAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAAACTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGAACCCGACCTTGGCAAGGTCGTGCTCTACCAAc > 2:375046/1‑135 (MQ=255) aacgaaAAAC‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACt < 6:263981/135‑1 (MQ=255) gaaAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg > 3:346932/1‑135 (MQ=255) acgcccccT‑AAGGGCTTTTTTTAAACGACTTACAGACTTCGACTCAAACCTGTATATATTTATTTGGAGCGGCAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTAtt < 4:124736/131‑1 (MQ=18) cgggCTTTTTTTAAACTCATTACACACTTCGACTGAAACCTGTATATGTTTATTTGGAGCGGTAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgtcgt < 4:58540/134‑1 (MQ=17) | AGCGATATGTCCAGGTAGCCCACGGCGTCACGGCCAGGGCGCCGCGTTGTGGTGTTTGTACTTGAATTTCGTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAA‑GGCCCCG‑AAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTCGT > KT2440/4626025‑4626281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |