BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,374,862	(T)_8→9	intergenic (+40/‑172)	pspE → / → ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,374,854	1	.	T	97.5%	2261.1 / 42.6	675	intergenic (+32/‑180)	pspE/ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand): ref base . (13/4); new base T (419/232); total (434/241)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 708 total aligned reads displayed.

GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGC  >  NZ_CP025268/1374716‑1374988

gACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑t                                                                                                                                          >  2:4510790/1‑140 (MQ=255)

  cGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑t‑tt                                                                                                                                       >  1:4845381/1‑140 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTTTGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         <  1:10023474/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         <  1:6908590/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         <  1:199249/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         <  1:9304437/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         <  2:2236870/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         <  2:9824409/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         <  1:5236277/140‑1 (MQ=255)

               aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCGTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         <  1:239921/140‑1 (MQ=255)

                agccaACGAGAACCTTAGCGAGATGGGATATACCCCCGTTGACAACGCCGGTGGCCTGAAACACCACGCACAGCCGAAGGTCAAAGGTTAAAAGAATACACCAAGCGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcaca                                                                                                                        <  2:7534038/140‑1 (MQ=255)

                agcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcaca                                                                                                                        <  2:6695571/140‑1 (MQ=255)

                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCacac                                                                                                                       >  1:1175434/1‑140 (MQ=255)

                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCacac                                                                                                                       >  2:4259963/1‑140 (MQ=255)

                 gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCAGGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCacac                                                                                                                       >  2:8300013/1‑140 (MQ=255)

                  caaAATAGATCCTTAGCGAGATGGGATATACCCACGTTGATCCCTCCGTTGGCCTGAAACACATCTCAATGACGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcacaac                                                                                                                      <  2:2880011/140‑3 (MQ=255)

                  caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACAcc                                                                                                                      <  1:847753/140‑1 (MQ=255)

                  caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACAcc                                                                                                                      <  1:6227922/140‑1 (MQ=255)

                         gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTa                                                                                                               >  2:1504832/1‑140 (MQ=255)

                         gaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTa                                                                                                               <  1:4269024/140‑1 (MQ=255)

                           tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACa                                                                                                             <  1:7435457/140‑1 (MQ=255)

                           gccTTAGCGAGATGGGATATACCCACGTTGAGCACGCCGGTGGCCTGACAGACAGCGCAATGCCGTCGGTCATAGGTTAAAAGATTTCACCAGGAGCGGCGCTGATGCGCCG‑‑TGTGTT‑TTTCT‑GTCACCCCGGATTGACa                                                                                                             >  2:3579787/2‑140 (MQ=255)

                            ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         >  1:8277976/1‑127 (MQ=255)

                            ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACAt                                                                                                            >  2:3201517/1‑140 (MQ=255)

                            ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACAt                                                                                                            <  2:5584773/140‑1 (MQ=255)

                            ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACAt                                                                                                            <  1:3154471/140‑1 (MQ=255)

                               tAGCGAGATGGGATATACCCACGTTGAGACCGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTGATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATccc                                                                                                         >  1:10825921/1‑140 (MQ=255)

                               tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATccc                                                                                                         <  1:9885492/140‑1 (MQ=255)

                                 gCGAGATGGGATAGACCCACGTGGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGTTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCa                                                                                                       <  2:8628480/140‑1 (MQ=255)

                                  cGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTAGTTAAATCCCCAt                                                                                                      >  1:5887442/1‑140 (MQ=255)

                                     gaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGa                                                                                                   <  2:3836808/140‑1 (MQ=255)

                                     gaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGa                                                                                                   <  1:6116257/140‑1 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACAcc                                                                                                                      >  2:5434976/1‑120 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGat                                                                                                  >  2:6019252/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGat                                                                                                  >  1:10415550/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTAT‑GTCACACCTTATTTACATACCCATAGat                                                                                                  >  2:2103000/1‑140 (MQ=255)

                                      aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑t‑tttt‑ttttctgt                                                                                                                            >  1:6669213/1‑109 (MQ=255)

                                          gATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAATAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGattatt                                                                                              <  1:4048704/140‑1 (MQ=255)

                                              taCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCg                                                                                          <  1:2559940/140‑1 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                         <  2:2422641/140‑1 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                         >  2:7365552/1‑140 (MQ=255)

                                               aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                         >  2:6921099/1‑140 (MQ=255)

                                                   aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGc                                                                                     >  2:8463358/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGTCATCGATCTGCGGAAGGTCAAAGGTTAAAAGAATTCACCATGTGCGGCGCTTTTGCGTCG‑‑T‑TTTTGTTTCT‑GTCAATCGTTTTTTTCATCCCCTGAGATTTTTTGCGTccggg                                                                                    >  2:2010424/1‑136 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTCAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCGTATTTAAATCCCCATAGATTATTTGCGTCAGCt                                                                                    >  2:4888729/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑TTCACACCATATTTACAACCCCATACATTATTTGCGGCAGCt                                                                                    >  1:7085000/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                    >  1:8639449/1‑140 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCACCATAGATTATTTGCGTCAGCt                                                                                    <  1:9586614/140‑1 (MQ=255)

                                                    cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCGTATTTACATCCCCATAGATTATTTGCGTCAGcg                                                                                    >  1:7959100/1‑139 (MQ=255)

                                                     gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACAt                                                                                                            <  1:8909882/115‑1 (MQ=255)

                                                               ccGGTGGCCTGAAAGACATCGCAATGCCGACGGTCAAAGGTTAAAAGAATTCACCAGGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGc                                                                         <  2:400521/140‑1 (MQ=255)

                                                               ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATa                                                                                                     >  2:1310343/1‑112 (MQ=255)

                                                               ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGc                                                                         >  1:5626797/1‑140 (MQ=255)

                                                                     gCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                         >  2:5159713/1‑118 (MQ=255)

                                                                        tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCA‑GTCACACCATATTTAAAAACCCAAAGATTATTTTAGTCAGCTCACAAATACCCATTTTACCt                                                                >  1:5515980/1‑140 (MQ=255)

                                                                               cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaa                                                         >  2:7969283/1‑140 (MQ=255)

                                                                               cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TGTTTT‑TTTAT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaa                                                         >  1:1846491/1‑140 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTCAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa                                                        >  2:7131309/1‑140 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                         <  1:4522763/107‑1 (MQ=255)

                                                                                aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaa                                                        >  2:9698259/1‑140 (MQ=255)

                                                                                      ttGCCGAAGGTCAATGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGAtt                                                  <  2:1200094/139‑1 (MQ=255)

                                                                                       tGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTT‑TTTT‑TTTCT‑GTCACACCGTATTTAAAACCCCCTAGATTATTTGCGTCAGCGCACAAATACGCTTTTTCCCTGGTAAAAAATGAtt                                                  >  1:226809/1‑140 (MQ=255)

                                                                                         ccGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTcc                                               >  2:4700092/1‑140 (MQ=255)

                                                                                            aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTcac                                                                                                                         >  1:10619486/1‑63 (MQ=255)

                                                                                            aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAg                                                                                      <  1:4725013/98‑1 (MQ=255)

                                                                                            aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc                                            >  1:3730681/1‑140 (MQ=255)

                                                                                            aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc                                            <  1:773887/140‑1 (MQ=255)

                                                                                            aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTACTGc                                            >  2:10386905/1‑140 (MQ=255)

                                                                                             aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTT‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc                                            <  1:3374274/140‑1 (MQ=255)

                                                                                                 cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTTCATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGAc                                       <  2:8938492/140‑1 (MQ=255)

                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTa                                     >  2:3352630/1‑140 (MQ=255)

                                                                                                   aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑T‑TTTT‑TTTTTGGTAACCCCTTATTTAAATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAATTGTTTTCCTGCGTGACTa                                     >  2:10105296/1‑140 (MQ=255)

                                                                                                        tAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAGAcc                                >  2:2588615/1‑140 (MQ=255)

                                                                                                        tAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTAAATAACCATAGATTATTTGCGTCAGCTCACAAATAAGCTTTTTCACTGGTAAAAAATGTTTTACTGCGTGTCTaaaaac                                >  2:4400613/1‑138 (MQ=255)

                                                                                                         aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACCCCATATTTAAATCCCCAAAGATTATTTTCGTCAGCTCACAAATACCCTTTTTCCCGGGTAAAAAATGATTTACTGCGTGACTAAAAccc                               >  1:3542396/1‑140 (MQ=255)

                                                                                                         aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTAT‑GTAACACCTTATTTACAACCCCATAGAATATTTGCGTCAGCTCACAAATAAGATTTTTCCCAGGTAAAAAATGATTTCCTGCGTGAAAAAAAccc                               >  1:6339631/1‑140 (MQ=255)

                                                                                                          aaaGAATTCACCATGAGCGGCGCTTATGCGCCGTAT‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                               <  2:10633273/140‑1 (MQ=255)

                                                                                                          aaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGGAAAAAATGATTTCCTGCGTGACTAAAACCCt                              >  2:10846148/1‑140 (MQ=255)

                                                                                                          aaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTAT‑GTCACACCTTATTTACATACCCATATATTATTTGCGTCAGCTCAAAAATAAGCTTTTTCCCTGGTAAAAAATTATTTCCAGCGTGTCGAAAAccca                              >  1:1417869/1‑139 (MQ=255)

                                                                                                               aTTCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGc                         <  1:9730211/140‑1 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGTTT‑TTTT‑TTTCT‑GTCACACCATATTTACATCCCCCTAGATTATTTGCGTCCGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTAGTGc                         >  2:9446658/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTTCTGCGTGACTAAAACCCTTGTGCt                        <  1:6405627/140‑1 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                        >  2:9062753/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                        >  2:8100476/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTACCTGGTAAAAAATGATTTCCTGCGTGACTAAAAACCTTGTGCt                        >  2:6132872/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAAAACGCATTTTCCCAGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGca                        >  1:754594/1‑139 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCATATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                        >  1:7298103/1‑140 (MQ=255)

                                                                                                                ttCACCATGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTAT‑GTCACACCTTATTTACAACCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTACCAGGTAAAAAATGATTTACTGCGTGACTAAAACCCTTGTGCt                        >  2:5750652/1‑140 (MQ=255)

                                                                                                                       tGAGCGGCGCTTCTGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCATGTGCTCAATTGa                 >  2:10255779/1‑140 (MQ=255)

                                                                                                                       tGAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGa                 >  2:6507762/1‑140 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACCCCTTATTTACATCCCCATAGTTTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                >  2:4561804/1‑140 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                >  2:1768787/1‑140 (MQ=255)

                                                                                                                        gAGCGGCGCTTATGCGCCGT‑T‑TTTT‑TTTAT‑TTAAAACCTTATTTAAATCCCCATATATTATTTTCGTCAGCTAAACAATAAGCTTTTTACCTGGGAAAAAATGATTTACTTCGTGACTAAAAACCAGGTGCTAAATTGaa                >  2:955645/1‑139 (MQ=255)

                                                                                                                             gcgcTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTAATTTCGTCATCACACAAATAAGCTTTTTCCCAGGTAAAAAATGATTTACTGAGTGAATAAAAACAATGTGCTCAATTTAAAGttt           >  2:8195178/1‑140 (MQ=255)

                                                                                                                              cgcTTATGCGCCGTTT‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGttt           >  2:6832022/1‑140 (MQ=255)

                                                                                                                               gcTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTGACACCTTATTTACATCCCCATATATTATTTGCGTCAGCTAACAAAAAAGCTTTTTACCTGGTAAAAAATTATTTACTGCGTGACTAAAACCCATGTTCTCAATTTACAGTTTAt         >  1:2799559/1‑140 (MQ=255)

                                                                                                                               gcTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAt         >  2:9156606/1‑140 (MQ=255)

                                                                                                                               gcTTATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTACCTGGTAAAAAATTATTTCCTGCGTGACTAAAAACCTTGTGCTCAATTGACAGTTTAt         >  1:9076783/1‑140 (MQ=255)

                                                                                                                                  tATGCGCCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtttt      <  1:3961952/140‑1 (MQ=255)

                                                                                                                                     gcgcCGT‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCATGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg   >  2:6271152/1‑140 (MQ=255)

GACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑T‑TTTT‑TTTCT‑GTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGC  >  NZ_CP025268/1374716‑1374988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`