BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	460,292	A→G	785 (TAA→TAG)	lon →	endopeptidase La

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	460,292	0	A	G	99.6%	1836.9 / ‑5.1	529	785 (TAA→TAG)	lon	endopeptidase La
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (179/346); minor base T (0/2); total (179/350)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 552 total aligned reads displayed.

TAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGATGGCTGGC  >  NZ_CP025268/460157‑460428

     gcgATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTcgc                                                                                                                                   <  1:845846/140‑1 (MQ=255)

          cTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCaaa                                                                                                                              <  1:10079895/140‑1 (MQ=255)

               agagATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                                                                                                                         <  2:90204/140‑1 (MQ=255)

               agagATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                                                                                                                         <  2:3326024/140‑1 (MQ=255)

               agagATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                                                                                                                         <  2:3033764/140‑1 (MQ=255)

               agagATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                                                                                                                         <  2:2148215/140‑1 (MQ=255)

               agagATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                                                                                                                         <  2:1353776/140‑1 (MQ=255)

               agagATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                                                                                                                         <  1:1490090/140‑1 (MQ=255)

               agagATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                                                                                                                         <  1:784084/140‑1 (MQ=255)

               agagATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                                                                                                                         <  1:4850099/140‑1 (MQ=255)

                   aTTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaa                                                                                                                     >  2:9625125/1‑140 (MQ=255)

                   aTTCCTGACAACGTAATAGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaa                                                                                                                     >  1:2423306/1‑140 (MQ=255)

                    ttCCTGACACCGTAAGTGCCGAGCTGGACCTTCATCCTGTGATGCGCATTGTGGTAGTTCTGACTCTGGCGCTGGATATTGATCCGTCTGGTATGCAGGTTGTGTCTGCAAAATAGTGTGCTCGCGCTAAATGCAAtaag                                                                                                                    <  2:10714727/140‑2 (MQ=255)

                    ttCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaat                                                                                                                    <  1:10445677/140‑1 (MQ=255)

                     tCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaata                                                                                                                   >  2:7197888/1‑140 (MQ=255)

                     tCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaata                                                                                                                   >  2:10033423/1‑140 (MQ=255)

                     tCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaata                                                                                                                   >  1:5704798/1‑140 (MQ=255)

                     tCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACtaata                                                                                                                   <  1:5197581/140‑1 (MQ=255)

                        tGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATaaaa                                                                                                                >  2:7626318/1‑140 (MQ=255)

                         gACATCGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTTTGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAATATGCACTAATaaaaa                                                                                                               <  2:7455216/140‑1 (MQ=255)

                             aCGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAgg                                                                                                           >  1:9046556/1‑140 (MQ=255)

                              cGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAggg                                                                                                          >  1:892933/1‑140 (MQ=255)

                                     gCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAg                                                                                                   <  1:2704562/140‑1 (MQ=255)

                                           cTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAAt                                                                                             <  2:4710794/140‑1 (MQ=255)

                                               aCATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAACTGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCACAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCgg                                                                                         <  2:38902/140‑1 (MQ=255)

                                                   tCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCtt                                                                                     >  2:8665942/1‑140 (MQ=255)

                                                    cATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTg                                                                                    >  1:7437493/1‑140 (MQ=255)

                                                     aTCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGc                                                                                   >  1:6390887/1‑140 (MQ=255)

                                                     aTCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGc                                                                                   <  1:6940201/140‑1 (MQ=255)

                                                     aTCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGc                                                                                   <  1:7195303/140‑1 (MQ=255)

                                                     aTCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGc                                                                                   >  1:7356710/1‑140 (MQ=255)

                                                         tgtgAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGc                                                                               <  2:10807251/140‑1 (MQ=255)

                                                          gtgAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGcc                                                                              <  2:6472515/140‑1 (MQ=255)

                                                           tgAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑t                                                                            >  1:6590051/1‑140 (MQ=255)

                                                             aaGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑ttt                                                                          >  1:7912344/1‑140 (MQ=255)

                                                                cgcATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCACAATGCACTAATAAAAACAGGGCTGGCAGTCTAATTCGGGCTTGCCAGCC‑tttttt                                                                       >  2:1263638/1‑140 (MQ=255)

                                                                 gcATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑ttttttt                                                                      >  2:2840348/1‑140 (MQ=255)

                                                                  cATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTg                                                                     >  2:6220197/1‑140 (MQ=255)

                                                                   cttGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGGTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGTCTAATTCGGGCTTGCCAGCC‑TTTTTTTGt                                                                    <  2:8122227/139‑1 (MQ=255)

                                                                   aTTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGt                                                                    <  2:8748214/140‑1 (MQ=255)

                                                                   aTTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGt                                                                    <  1:1078789/140‑1 (MQ=255)

                                                                   aTTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGt                                                                    <  1:2878934/140‑1 (MQ=255)

                                                                   aTTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGt                                                                    <  1:3945871/140‑1 (MQ=255)

                                                                     tGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGtct                                                                  >  1:10723731/1‑140 (MQ=255)

                                                                      gAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGtctc                                                                 <  2:1664183/140‑1 (MQ=255)

                                                                       aGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCg                                                                <  2:1036146/140‑1 (MQ=255)

                                                                       aGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCg                                                                <  1:2005605/140‑1 (MQ=255)

                                                                       aGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAACC‑TTTTTTTGTCTCg                                                                <  2:2377986/140‑1 (MQ=255)

                                                                         gAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCt                                                              <  1:7008432/140‑1 (MQ=255)

                                                                             ttCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGt                                                          <  1:4007770/140‑1 (MQ=255)

                                                                              tCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGtt                                                         <  1:245376/140‑1 (MQ=255)

                                                                                  aCTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAt                                                     <  1:5479675/140‑1 (MQ=255)

                                                                                    tctGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑gg                                                  <  1:6041820/140‑1 (MQ=255)

                                                                                    tctGGCGCTGCAAAATGAACCGTCTGGCATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑gg                                                  <  2:11526/140‑1 (MQ=255)

                                                                                       ggCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCgg                                               >  2:5828649/1‑140 (MQ=255)

                                                                                         cgcTGCAACATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAATCAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGAt                                             <  2:5708948/140‑1 (MQ=255)

                                                                                         cgcTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGAt                                             <  1:1685555/140‑1 (MQ=255)

                                                                                         cgcTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGAt                                             <  2:4133107/140‑1 (MQ=255)

                                                                                              cAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGc                                        >  1:9497520/1‑140 (MQ=255)

                                                                                                 aaTGTACCGTCTGGTATGCAGGTTGTTACTGCAAAATAGTGACATCGCTCAAAATGCACTAATAAAATAAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TATTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTg                                     <  2:6771950/140‑1 (MQ=255)

                                                                                                 aaTGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTg                                     <  2:5223565/140‑1 (MQ=255)

                                                                                                 aaTGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTg                                     <  1:3742743/140‑1 (MQ=255)

                                                                                                 aaTGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTg                                     <  2:3493352/140‑1 (MQ=255)

                                                                                                  aTGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGc                                    <  2:3019643/140‑1 (MQ=255)

                                                                                                   tGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGcc                                   <  1:3433228/140‑1 (MQ=255)

                                                                                                    gAACCGTCTGGTATGCAGGTAGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAACCAGGGCTGGCAGGCTAATTCTGGCTTGCCAGCC‑TTTTTTTGTCTCGATAAGTTAGAT‑GGCGGTTCGGGCTTGccc                                  <  2:1743684/140‑1 (MQ=255)

                                                                                                      aCCGTCTGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCa                                                                                                                         >  2:5931182/1‑53 (MQ=255)

                                                                                                            tGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAg                          <  1:5828649/140‑1 (MQ=255)

                                                                                                            tGGTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAg                          <  1:3200477/140‑1 (MQ=255)

                                                                                                              gTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAggg                        <  1:178184/140‑1 (MQ=255)

                                                                                                              gTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAggg                        <  2:4317464/140‑1 (MQ=255)

                                                                                                              gTATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAggg                        <  2:5077988/140‑1 (MQ=255)

                                                                                                               tATGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑ATTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAgggg                       <  1:3506288/140‑1 (MQ=255)

                                                                                                               tATGCAGGTTGTGACTGCAAAATAGTGACCGCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGATAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTTCCCTTATTAAgggg                       <  2:9294263/140‑1 (MQ=255)

                                                                                                                aTGCAGGTTGTGACTGCAAACTAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCGTGCCCTTATTAAGGGgt                      <  2:1977909/140‑1 (MQ=255)

                                                                                                                aTGCAGGTTGTGACTGCAAAATAGGGACCTCGCGCAAAATGCACTAATACAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGgt                      <  1:4322223/140‑1 (MQ=255)

                                                                                                                 tGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGgtg                     <  1:3585069/140‑1 (MQ=255)

                                                                                                                 tGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGgtg                     <  1:7645966/140‑1 (MQ=255)

                                                                                                                 tGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGgtg                     <  2:5457197/140‑1 (MQ=255)

                                                                                                                 tGCAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAAGTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGgtg                     <  1:1293668/140‑1 (MQ=255)

                                                                                                                   cAGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGtgtt                   <  1:1748338/140‑1 (MQ=255)

                                                                                                                    aGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCCTTTTTTTTGACTGGATAA‑TTAGTTGGGCGGAACGGGATTGCCCTTATTATGGGGggtt                   >  2:8435751/1‑140 (MQ=255)

                                                                                                                    aGGTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCGTATTAAGGGGggttg                  >  1:8240663/1‑140 (MQ=255)

                                                                                                                     ggTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGtgttgt                 <  2:5370580/140‑1 (MQ=255)

                                                                                                                     ggTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCGTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAG‑GGCGGATCGGGCTGGCCCGTATTAAGGGGtgttgg                 >  1:4173275/1‑139 (MQ=255)

                                                                                                                      gTTGTGACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTa                <  2:609896/140‑1 (MQ=255)

                                                                                                                        tgtgACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAg              >  1:10344127/1‑140 (MQ=255)

                                                                                                                        tgtgACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAAAAGGTCTGGCAGGCTAATTAGGGCTTGCCAGCC‑TTGTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCTGGCTTGCCGTTATTCAGGGgtgtgggatg              >  2:7973877/1‑134 (MQ=255)

                                                                                                                         gtgACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGa                                              <  1:10001177/107‑1 (MQ=255)

                                                                                                                          tgACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTACGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAggg            <  1:6172084/140‑1 (MQ=255)

                                                                                                                          tgACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAggg            <  1:9769245/140‑1 (MQ=255)

                                                                                                                          tgACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAggg            <  1:807773/140‑1 (MQ=255)

                                                                                                                          tgACTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAG‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAggg            <  2:8303911/140‑1 (MQ=255)

                                                                                                                           gACTGCAACATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTATATTGTCTCGCTAAGTTAGAT‑TGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAgggg           <  2:10486420/140‑1 (MQ=255)

                                                                                                                            aCTGCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGa          <  2:253913/140‑1 (MQ=255)

                                                                                                                               gCAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGAtgg       <  1:4553674/140‑1 (MQ=255)

                                                                                                                                cAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGAtggc      >  2:7721602/1‑140 (MQ=255)

                                                                                                                                cAAAATAGTGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGAtggc      <  1:9869350/140‑1 (MQ=255)

                                                                                                                                   aatagtGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTCAGGGGTGTTGTAAGGGGAtggctgg   <  2:10186560/140‑1 (MQ=255)

                                                                                                                                   aatagtGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGAtggctgg   <  1:8673821/140‑1 (MQ=255)

TAAACGCGATCTGGAAGAGATTCCTGACAACGTAATTGCCGATCTGGACATTCATCCTGTGAAGCGCATTGAGGAAGTTCTGACTCTGGCGCTGCAAAATGAACCGTCTGGTATGCAGGTTGTGACTGCAAAATAATGACCTCGCGCAAAATGCACTAATAAAAACAGGGCTGGCAGGCTAATTCGGGCTTGCCAGCC‑TTTTTTTGTCTCGCTAAGTTAGAT‑GGCGGATCGGGCTTGCCCTTATTAAGGGGTGTTGTAAGGGGATGGCTGGC  >  NZ_CP025268/460157‑460428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`