| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,228,222 | 0 | T | G | 62.5% | 0.1 / 11.6 | 16 | pseudogene (388/1287 nt) | mdtQ | pseudogene; putative channel/filament proteins |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (10/0); total (13/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.94e-02 | |||||||||||
AAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCT‑CGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGA‑GAT‑GCC‑AGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGG > NC_000913/2228075‑2228349 | aagTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCCGCCAGCAATTGGCGGGGTTg > 2:15065/1‑151 (MQ=255) cTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTTCT‑CGCGTTccgc > 1:57178/1‑150 (MQ=255) gATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCCACGCCGCCAGCCCGCAATTGGCGGGGTTgtccccggttccccc > 1:214493/1‑136 (MQ=255) gTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCC‑CGCGTTTCGCCGCCCCTGCTTTaaaccta > 1:377748/1‑145 (MQ=255) tCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCT‑CGCGTTCCGCCGCCCGTGCTTTAACCGTAcc < 2:296135/151‑1 (MQ=255) aTAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCT‑CGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAgg < 2:29629/150‑1 (MQ=255) aaTGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTGCT‑CCCGTTCCCCCCCCCGTGCTTTTACCCCACCCCGgggg > 2:99642/1‑151 (MQ=255) cTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCT‑CGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAAcc < 2:89010/151‑1 (MQ=255) gCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCCGCAATTGGCGGGGTTGCT‑CCCGTTCCGCCGCCCCTGCCTTTACCCGACCCCGGCGGGCAGTAACCt > 1:372726/1‑150 (MQ=255) gtTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGGTGCCAGCCCGCAATTGGCGGGGTTTAC‑CGGGGTTCGCAGCCCGGGGTTTAACCGGACCCAGGCGGGGAGCAACCCCCCCCCCCCTTTTTCCCCAGATATaga‑gg > 2:205336/1‑150 (MQ=255) gtTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCT‑CGCGGTCCGCCGCCCCGGCTTTTAACGTACCCCAGCGGGCAGGAACCCCCCCCCCCTTCTTTTCCCCGagattcagg > 1:149572/1‑146 (MQ=255) gCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTGCTCCCCGTTCC‑CCGCCCCCGCTTTAAACCGACCCAGGCGGGGCGTAACCTCCGCCCGATTCTTTTCCCCGATATCCA‑GAT‑GCC‑AGcccccccgttagc > 1:66813/1‑143 (MQ=255) cTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTTCT‑CGCGGTCCGCCGCCCCGGCTTTTACCCCACCCCGGCGGGCAGTAAACTCCGCCCCGTTCTTTTCCCCGATATCCAGGAT‑CCC‑A‑CCCGCCCTTAAACCcaaa > 1:287798/1‑151 (MQ=255) gTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTTT‑CCGGTTCCGCCGCCCGTGCTTTAACCGTACCCCGGGGGGGGGGAAACCCCGCCCCGTTCTTTTCCCCGATATCCG‑GAT‑GCCAACCCCCCCGTTAAGCCAAAAGAAcg > 2:47413/1‑150 (MQ=255) gTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGGTTTCT‑CCCGGTCCCCCGCCCCGGCCTTTAACGCAACCCGGGGGGGAGGAACCCCCCCCCCATTCTTTCCCCCGATATCGA‑GATGGCA‑ACCCCCCCGTTAACCCaaaaaaacg > 1:308969/1‑146 (MQ=255) cTGCCAGCCAGCAATTGGCGGGTTTGCG‑CGCGTTCCGCCGCCCGTGCTTTAACCCTACCCCGGGGGGCAGGAAACTCCGCCCCACTCTTTCCCCAACAATCGA‑CAT‑GGC‑ATCCCGCCGTTCACCCCAAAGTACCGTTCGTGTACCCCggg > 1:255625/1‑150 (MQ=255) | AAGTAATCCCGTTCTGATATAGCTGGCGATCGGTCGCGATAATGGTGTTCTGCTCTTTTTCTATTTGCTGCAAGACCGTGTTTAACGCCGCCTGGGTTTGCCACTCCCAGTACAGGCGGGCTACGCTGCCAGCCAGCAATTGGCGGGTTTGCT‑CGCGTTCCGCCGCCCGTGCTTTAACCGTACCCAGGCGGGCAGTAACCTCCGCCCGATTCTTTCCCCAGATATCGA‑GAT‑GCC‑AGCCCGCCGTTAAGCCAAAAGTACCGTTGGTGTACCACGGG > NC_000913/2228075‑2228349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |