| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,850,810 | (C)5→4 | intergenic (+117/‑50) | sppA → / → ansA | protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,850,806 | 0 | C | . | 97.6% | 164.5 / ‑2.4 | 41 | intergenic (+113/‑54) | sppA/ansA | protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1 |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (21/19); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG > NC_000913/1850670‑1850940 | cTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTccc < 1:339916/139‑1 (MQ=255) tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCt < 2:797149/139‑1 (MQ=255) tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCt < 1:539680/139‑1 (MQ=255) cgcCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCa < 1:216053/139‑1 (MQ=255) cATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGtata > 2:185278/1‑139 (MQ=255) cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACt > 2:481594/1‑139 (MQ=255) tCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGctc > 2:665139/1‑139 (MQ=255) cTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACTAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGctct < 1:267052/139‑1 (MQ=255) tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCttt < 1:350346/139‑1 (MQ=255) tGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGata < 2:723123/139‑1 (MQ=255) aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc > 2:595921/1‑139 (MQ=255) aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGACCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc > 2:146980/1‑139 (MQ=255) ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt < 1:665139/139‑1 (MQ=255) ggCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATg < 1:481594/139‑1 (MQ=255) ccACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat > 2:640475/1‑127 (MQ=255) ccACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatat < 1:640475/127‑1 (MQ=255) acaTTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCaaa < 1:185278/139‑1 (MQ=255) tACGTCGACGAATCCACTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAAtt > 1:535132/1‑139 (MQ=255) gTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGata < 2:134160/101‑1 (MQ=255) gTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGata > 1:134160/1‑101 (MQ=255) cgacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGt > 1:106068/1‑139 (MQ=255) cgacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGt > 1:698216/1‑139 (MQ=255) cccATATTTCCTTATCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGAt > 2:201440/1‑139 (MQ=255) aTCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg < 2:788821/139‑1 (MQ=255) aTCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg < 2:228820/139‑1 (MQ=255) aTCCGCATAGTATCAGGTGCACT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAg < 1:146980/139‑1 (MQ=255) tCCGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGc > 1:361563/1‑139 (MQ=255) ccGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCa > 1:363634/1‑127 (MQ=255) ccGCATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCa < 2:363634/127‑1 (MQ=255) ccGCAGAGTTACAGGTGCGC‑GCCCCTGCCTCGCGTATATGATGTTCTTCTTCCGATATCATCCATACCAATATCATGCAAAAGAAATCATTTTACGATGCCTACACGGGCGGGACGATCGGGATGCAGCGTTCCGAGCa < 1:129146/139‑1 (MQ=255) cATAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAggg > 2:19766/1‑139 (MQ=255) aTAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTac > 2:369802/1‑100 (MQ=255) aTAGTATCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTac < 1:369802/100‑1 (MQ=255) aTCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa > 1:703390/1‑130 (MQ=255) aTCAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCa < 2:703390/130‑1 (MQ=255) cAGGTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATAc > 2:4793/1‑139 (MQ=255) ggTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt < 2:561434/49‑1 (MQ=255) ggTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt > 1:561434/1‑49 (MQ=255) gTGCGCT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCgg > 2:271291/1‑139 (MQ=255) gcgcTCCG‑CTGCCTCGCGTATATACTTTTGCTCTTTCGATATCATTCGTATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGACCAGGGATATCTACCGgtg > 1:387757/1‑139 (MQ=255) gcgcT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt > 2:597186/1‑46 (MQ=255) gcgcT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt < 1:597186/46‑1 (MQ=255) gcgcT‑CCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgtg > 2:120261/1‑139 (MQ=255) | CTGACCTGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGTG > NC_000913/1850670‑1850940 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |