| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,250,629 | (C)5→6 | coding (210/1071 nt) | dhaK ← | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,250,624 | 1 | . | C | 96.2% | 83.7 / ‑3.2 | 26 | coding (215/1071 nt) | dhaK | dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (6/19); total (6/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGA > NC_000913/1250486‑1250752 | tCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAgg < 1:246725/139‑1 (MQ=255) cGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑c > 2:407242/1‑139 (MQ=255) gTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGAGAGGTGAAAATTTCGCCCGGACAGG‑ccc > 1:729196/1‑139 (MQ=255) ttCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAg < 2:251218/139‑1 (MQ=255) aGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGa < 2:444290/139‑1 (MQ=255) aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:26808/139‑1 (MQ=255) aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:680199/139‑1 (MQ=255) aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 1:728784/139‑1 (MQ=255) aaTATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTAGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:611623/139‑1 (MQ=255) ccGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc > 1:667732/1‑132 (MQ=255) ccGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:667732/132‑1 (MQ=255) gtAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACcaca < 2:260449/139‑1 (MQ=255) aGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCg < 2:24862/139‑1 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAc < 2:488614/100‑1 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAc > 1:488614/1‑100 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc > 2:192937/1‑139 (MQ=255) cTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgccgc < 1:799981/139‑1 (MQ=255) tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCTAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCa > 2:567229/1‑139 (MQ=255) tATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCAt > 1:291161/1‑139 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc < 2:155602/56‑1 (MQ=255) gCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGAcc > 1:155602/1‑56 (MQ=255) cGGTGAGGTGAAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGt < 1:376871/139‑1 (MQ=255) aggtgaAAATTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGACTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACAt < 1:459194/139‑1 (MQ=255) aaaaTTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 2:78391/139‑1 (MQ=255) tgttttCGACCGGGCAGGCCCC‑CCGAAAGCATCTCCTGACCGATATAACCACAGTGCATCGGGTCGTGTCCGCGGCCGCCTCACGACAGCAGGGCGACTTTTCCTGCAACATGGACAGCAGCGCGGGTGACATATACCg < 1:716784/136‑1 (MQ=255) aaaTTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 2:454933/139‑1 (MQ=255) aaaTTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 1:192937/139‑1 (MQ=255) aaaTTTCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAAAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCg < 1:784279/139‑1 (MQ=255) ttCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATc < 2:395894/139‑1 (MQ=255) ttCGCCCGGACAGGCCCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATc < 2:700435/139‑1 (MQ=255) cctggACAGG‑CCCGCCGAAAGCAACCCCTGAAAGATACAACCACAGGGCATCGGCTCGTCTCCGCTGCCGCCACCCGACAGAAGAGCGACTTTTCCTGCAACAGGGGCAACAGCTCGGGTGACAGAAAACGGATCCGGa < 1:567229/136‑1 (MQ=255) | TCGGTCGCTGTTTCAAAGTTAAGAATATCGCCGGTGTAATTTTTGATAATCAACAGTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCC‑CCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTTTCCTGCAACAGGGGCATCAGCTCGGGTGACATACACCGGATCCTGA > NC_000913/1250486‑1250752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |