| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,511,746 | (A)8→9 | coding (599/906 nt) | yhfZ ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,511,738 | 1 | . | A | 86.7% | 82.5 / 7.0 | 30 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (2/2); new base A (15/11); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA > NC_000913/3511601‑3511869 | cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCTCC‑a > 1:155530/1‑139 (MQ=255)cggcgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑a < 2:415378/139‑1 (MQ=255) cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaa‑a > 2:581139/1‑139 (MQ=255) aTCCCAGCATGGTCAATTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCgg < 2:342937/139‑1 (MQ=255) ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCa < 2:410339/139‑1 (MQ=255) aGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATg < 1:383853/139‑1 (MQ=255) tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCt > 2:158198/1‑139 (MQ=255) tCGTTTTCCGCCACCACGTTCCAGATCACCGCTTCGACATCGCCTTTAACAATGCGTTGTAACCTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaa‑aaaa‑aaacat < 2:50698/120‑6 (MQ=255) tCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑aaa‑aaaa‑aaacat > 1:50698/1‑115 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATccgccg < 2:85277/139‑1 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATccgccg > 1:71483/1‑139 (MQ=255) cGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACgg < 2:10592/139‑1 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 1:23877/1‑139 (MQ=255) aCATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGttt > 2:687449/1‑139 (MQ=255) aTGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTa > 1:789599/1‑139 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc > 1:454265/1‑139 (MQ=255) gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATccgccg < 2:306684/90‑1 (MQ=255) gCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATccgccg > 1:306684/1‑90 (MQ=255) tAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAA‑AAAATACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAAt > 2:79498/1‑139 (MQ=255) cGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGt > 1:586951/1‑139 (MQ=255) gTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 2:789599/139‑1 (MQ=255) aaGAGAGATCGACTCGTTCCACATCACTACGCCA‑AAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGcc < 1:739329/139‑1 (MQ=255) agaTCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCATCCCCAcg > 2:587904/1‑85 (MQ=255) agaTCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 1:587904/85‑1 (MQ=255) agaTCGACTCGTTCCACATCACTACCGCC‑AAACAAAA‑ACATCGGCCACGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCCAATCAACTGGTACTCGCCAACg > 1:245298/1‑139 (MQ=255) tCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATccgccg > 2:550919/1‑62 (MQ=255) tCGACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATccgccg < 1:550919/62‑1 (MQ=255) gACTCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGgt > 2:328597/1‑139 (MQ=255) tCGTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGtgtg < 2:801989/139‑1 (MQ=255) gTTCCACATCACTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg < 1:687449/139‑1 (MQ=255) aCTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAG‑‑GTGCGGCCCCAACTCCa > 2:237233/1‑139 (MQ=255) cTACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCACCGTAGGTGTGCGGCCCCAACTcc > 1:172471/1‑139 (MQ=255) tACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGc > 1:323883/1‑85 (MQ=255) tACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGc < 2:323883/85‑1 (MQ=255) tACCGCCAAAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 1:9082/1‑139 (MQ=255) | CGGCGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑AAA‑AAAA‑ACATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCA > NC_000913/3511601‑3511869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |