| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,980,957 | (G)6→7 | coding (71/1386 nt) | yifK → | putative APC family amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,980,951 | 1 | . | G | 98.3% | 217.3 / ‑3.8 | 60 | coding (65/1386 nt) | yifK | putative APC family amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (32/27); total (32/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAA > NC_000913/3980827‑3981082 | gATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCAtt > 2:312036/1‑139 (MQ=255) cccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTc > 1:114851/1‑139 (MQ=255) ccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCg < 1:426844/139‑1 (MQ=255) cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCgg > 1:681411/1‑139 (MQ=255) aTAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGc < 2:205926/139‑1 (MQ=255) tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGcc > 1:86286/1‑139 (MQ=255) tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGcc > 1:437730/1‑139 (MQ=255) gATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGtt > 2:280932/1‑139 (MQ=255) tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTa > 1:738717/1‑139 (MQ=255) aaCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTAt > 1:337260/1‑139 (MQ=255) aaCCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTAt > 2:685991/1‑139 (MQ=255) ggTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATgggg < 2:681411/139‑1 (MQ=255) tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgc > 1:260273/1‑139 (MQ=255) aaCACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgcc > 2:680047/1‑139 (MQ=255) cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc > 1:34997/1‑139 (MQ=255) cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc < 1:337101/139‑1 (MQ=255) cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc > 1:420669/1‑139 (MQ=255) ccGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:767412/139‑1 (MQ=255) cGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg > 2:655836/1‑139 (MQ=255) tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCTTGGG‑GGGGCACCATTGGCGTCGGc > 2:754990/1‑110 (MQ=255) tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGc < 1:754990/110‑1 (MQ=255) tGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa < 1:586484/139‑1 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc < 1:238548/139‑1 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc > 2:231304/1‑139 (MQ=255) cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc > 2:579545/1‑139 (MQ=255) aggAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGGACCCTGAAATgggccgggcc < 1:470223/137‑1 (MQ=255) gATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg < 2:260273/139‑1 (MQ=255) tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 2:420669/139‑1 (MQ=255) tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 2:34997/139‑1 (MQ=255) tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt < 2:738717/139‑1 (MQ=255) acagtAACAAACCCGGGCTACAGCGTGGGCTGGAAGCTCGACATAACGAACTCATCGCCCT‑GGAGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATtggtgg < 1:514900/135‑1 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGc > 2:134803/1‑82 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGc < 1:134803/82‑1 (MQ=255) gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc > 2:596584/1‑139 (MQ=255) agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGc > 2:675136/1‑139 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg > 2:593452/1‑139 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATGGTTGGCCTATatcatcctgt > 2:514900/1‑135 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gggggca < 2:134245/55‑4 (MQ=255) agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑gg‑gggggca > 1:134245/1‑52 (MQ=255) cTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg < 1:312036/139‑1 (MQ=255) ggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa < 2:554939/76‑1 (MQ=255) ggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa > 1:554939/1‑76 (MQ=255) ggCTGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt > 2:397825/1‑139 (MQ=255) tGGAAGCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCtttt < 1:596584/139‑1 (MQ=255) gCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc < 2:273860/139‑1 (MQ=255) gCTCGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatc < 2:772122/139‑1 (MQ=255) cGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGc > 2:35584/1‑46 (MQ=255) cGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGc < 1:612885/46‑1 (MQ=255) cGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGc > 2:612885/1‑46 (MQ=255) cGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGc < 1:35584/46‑1 (MQ=255) cGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc < 2:799864/87‑1 (MQ=255) cGACATATCGAACTCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc > 1:799864/1‑87 (MQ=255) tCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg < 2:701215/53‑1 (MQ=255) tCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg > 1:701215/1‑53 (MQ=255) tCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg < 2:25624/87‑1 (MQ=255) tCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgttg > 1:25624/1‑87 (MQ=255) tCATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATTCGTTCAATGGGCg > 2:682869/1‑139 (MQ=255) cATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCCGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc > 2:125906/1‑72 (MQ=255) cATCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCCGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc < 1:125906/72‑1 (MQ=255) aTCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaa < 2:461789/139‑1 (MQ=255) aTCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaa > 1:603593/1‑139 (MQ=255) tCGCCCTGGG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaaa < 1:231304/139‑1 (MQ=255) | GATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑GG‑GGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAA > NC_000913/3980827‑3981082 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |