Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,936,931	T→C	F450L (TTC→CTC)	fucI →	L‑fucose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,936,931	0	T	C	100.0%	114.7 / NA	32	F450L (TTC→CTC)	fucI	L‑fucose isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

CGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCA  >  NC_000913/2936796‑2937066                                                                                                                                        |                                                                                                                                         cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTcc                                                                                                                                       >  2:550819/1‑139 (MQ=255) cgcgACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTcc                                                                                                                                       >  2:280181/1‑139 (MQ=255)           aaGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑Agg                                                                                                                            >  2:52503/1‑139 (MQ=255)                   ccGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTcc                                                                                                                    <  1:345259/139‑1 (MQ=255)                                       ggAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTc                                                                                                >  1:290682/1‑139 (MQ=255)                                                  aGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCaa                                                                                     <  2:503132/139‑1 (MQ=255)                                                        agGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCt                                                                               >  1:392788/1‑139 (MQ=255)                                                             gACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  1:282290/139‑1 (MQ=255)                                                             gACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAc                                                                          <  2:80376/139‑1 (MQ=255)                                                              aCGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGAcc                                                                         >  2:450721/1‑139 (MQ=255)                                                                    gCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACt                                                                   >  1:513954/1‑139 (MQ=255)                                                                               aCCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCgg                                                        <  1:52503/139‑1 (MQ=255)                                                                                   aaTGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAgg                                                    >  1:774290/1‑139 (MQ=255)                                                                                        tGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGa                                               >  2:709195/1‑139 (MQ=255)                                                                                          cccGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGc                                             >  2:440119/1‑139 (MQ=255)                                                                                                gATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                                       <  1:586589/139‑1 (MQ=255)                                                                                                gATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGaa                                       <  2:790999/139‑1 (MQ=255)                                                                                                  tCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAAtt                                     >  1:574736/1‑139 (MQ=255)                                                                                                      cGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCg                                 <  2:513954/139‑1 (MQ=255)                                                                                                      cGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCg                                 <  2:574736/139‑1 (MQ=255)                                                                                                       gAATACTTCCGTGGCGGCCGTTACTCTTCCCGCCTCCTTACCGATCGGCGCCG‑CCCGTTGACCATAAGTGACGGCAACATCATGGAAGGCCTGTGACAGGTACTGCAAATCGCGGAAGGCTGGATCGTGTAATTGCCGa                                <  1:363438/139‑1 (MQ=255)                                                                                                        aaTACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGaa                               <  1:125096/139‑1 (MQ=255)                                                                                                          tACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAgg                             <  2:207217/139‑1 (MQ=255)                                                                                                          tACTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAgg                             <  1:450721/139‑1 (MQ=255)                                                                                                            cTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAt                           >  2:92381/1‑139 (MQ=255)                                                                                                            cTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAt                           >  2:695908/1‑139 (MQ=255)                                                                                                            cTTCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAt                           >  2:455558/1‑139 (MQ=255)                                                                                                              tCCGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGAtgt                         >  2:370142/1‑139 (MQ=255)                                                                                                               ccGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTccc                                                                                                                   >  2:56742/1‑48 (MQ=255)                                                                                                               ccGTGGCGGCGGTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTccc                                                                                                                   <  1:56742/48‑1 (MQ=255)                                                                                                                     cggcggTTACTCTTCCCGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGAc                  <  2:569357/139‑1 (MQ=255)                                                                                                                                     cGCCTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCa  >  2:181503/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                         CGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCTCAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATACTTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGA‑AGGCGGCGTCCCGTTCACCATGACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGCGTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCA  >  NC_000913/2936796‑2937066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑