Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	391,739	C→T	*289* (TAG→TAA)	insF1 ←	IS3 transposase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	391,739	0	C	T	100.0%	114.4 / NA	38	*289* (TAG→TAA)	insF1	IS3 transposase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/19);  total (19/19)

TGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCC  >  NC_000913/391618‑391844                                                                                                                           |                                                                                                          tGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGtt                                                                                          >  2:345858/1‑139 (MQ=255)       aGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCaaa                                                                                    <  1:414097/139‑1 (MQ=255)        gATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAAt                                                                                   >  2:641477/1‑139 (MQ=255)             cTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTc                                                                              >  1:554528/1‑139 (MQ=255)                  ttGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGAc                                                                         >  1:377705/1‑139 (MQ=255)                     aTACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGa                                                                      <  1:350838/139‑1 (MQ=255)                         cggtcagcTGTCTAGTAACTCCTAGAGTACTGTTGCGTTGACCGAATCTACTTGGTGGGGGGCTGATGG‑ACCCGACCCACGTAATATGGTCACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTCTGCCGGACTGAGgcc                                                                  <  1:13204/136‑1 (MQ=21)                           atcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTGAATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccg                                                                 <  1:498523/139‑1 (MQ=255)                           atcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgc                                                                >  2:783772/1‑139 (MQ=255)                           atcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgc                                                                <  1:641477/139‑1 (MQ=255)                            tcatcTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgccgcc                                                               >  1:666637/1‑139 (MQ=255)                                   aCTATTAACTCCTAGAGCACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATTCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCag                                                        <  2:493022/139‑2 (MQ=255)                                                 cAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCa                                          >  1:171378/1‑139 (MQ=255)                                                  aGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCAc                                         <  2:738891/139‑1 (MQ=255)                                                     aCTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGa                                      <  2:360724/139‑1 (MQ=255)                                                     aCTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGa                                      >  2:753481/1‑139 (MQ=255)                                                      cTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCaaa                                                                                    >  2:655679/1‑92 (MQ=255)                                                      cTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCaaa                                                                                    <  1:655679/92‑1 (MQ=255)                                                        gTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTg                                   >  2:80835/1‑139 (MQ=255)                                                         ttGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGt                                  >  2:18478/1‑139 (MQ=255)                                                         ttGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGt                                  >  1:20589/1‑139 (MQ=255)                                                         ttGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCACCACCGATTGt                                  >  2:519848/1‑139 (MQ=255)                                                              ttGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgcc                                                                  <  2:395468/102‑1 (MQ=255)                                                              ttGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGgcc                                                                  >  1:395468/1‑102 (MQ=255)                                                                gACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcaca                           <  1:48873/139‑1 (MQ=255)                                                                gACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATcaca                           <  2:20589/139‑1 (MQ=255)                                                                     aaTCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGa                      <  1:80835/139‑1 (MQ=255)                                                                     aaTCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGa                      <  2:554528/139‑1 (MQ=255)                                                                       tCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGata                    <  2:377705/139‑1 (MQ=255)                                                                       tCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGata                    <  1:18478/139‑1 (MQ=255)                                                                             gggtgggGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTa              >  1:632280/1‑139 (MQ=255)                                                                               gtgggGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTaaa            >  1:434554/1‑139 (MQ=38)                                                                                  gggggCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAacac         >  2:166629/1‑139 (MQ=37)                                                                                   ggggCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAAt                               >  2:325016/1‑116 (MQ=25)                                                                                   ggggCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAAt                               <  1:325016/116‑1 (MQ=25)                                                                                         gattg‑atCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGcc  <  2:62229/139‑1 (MQ=14)                                                                                         gattg‑atCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGcc  <  2:632280/139‑1 (MQ=14)                                                                                         gattg‑atCCTACCCACGTAATATGGACACAGGCTTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGcc  >  1:206981/1‑139 (MQ=14)                                                                                                                           |                                                                                                          TGGTGAAGATGGCTACGTTGATACCGATCATCTGACTATTAACTCCTACAGTACTGTTGCGTTGACCGAATCTACTGGGTGGGGGGCTGATTG‑ATCCTACCCACGTAATATGGACACAGGCCTAAGCGAGGTTCTTGTTTTCAAATTGTTCCGGACTGAGGCCGCCACACCAACTGTGCCGCCGCCACCGATTGTAATCACATTCGATATAATTAAACACCGTTGCC  >  NC_000913/391618‑391844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑